Click image to see more details
Product Info Summary
| SKU: | A01651-Dyl488 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Human SCRIBBLE DyLight® 488 conjugated Antibody
SKU/Catalog Number
A01651-Dyl488
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human SCRIBBLE DyLight® 488 conjugated Antibody catalog # A01651-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human SCRIBBLE DyLight® 488 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01651-Dyl488)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human SCRIBBLE recombinant protein (Position: F172-K409).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01651-Dyl488 is reactive to SCRIB in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
174.885kDa
Background of Scribble
SCRIB, also known as Scribble, SCRIBL, or Scribbled homolog (Drosophila), is a scaffold protein which in humans is encoded by the SCRIB gene. In Drosophila melanogaster, SCRIB is involved in synaptic function, neuroblast differentiation, and epithelial polarization. Mechanistically, the human homolog is a scaffold protein linked to cellular differentiation centered on the regulation of epithelial as well as neuronal morphogenesis. Deficiency in SCRIB impairs many aspects of cell polarity and cell movement. SCRIB is also likely involved in establishing apical-basal polarity as well as progression from the G1 phase to S phase in the cell cycle as a result of its relationship with cell proliferation and exocytosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01651-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Positive Control
FCM: HepG2 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Flow Cytometry analysis of HepG2 cells using human SCRIBBLE DyLight® 488 conjugated Antibody (A01651-Dyl488).
Overlay histogram showing HepG2 cells stained with A01651-Dyl488 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FBXL4 Antibody (A01651-Dyl488,8-10μL/1x106 cells) for 30 min at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For SCRIB (Source: Uniprot.org, NCBI)
Gene Name
SCRIB
Full Name
Protein scribble homolog
Weight
174.885kDa
Superfamily
LAP (LRR and PDZ) protein family
Alternative Names
Protein scribble homolog; Scribble; hScrib; Protein LAP4; SCRIB; CRIB1, KIAA0147; LAP4; SCRB1; VARTUL SCRIB CRIB1, SCRB11, Vartul, SCRIB scribble planar cell polarity protein protein scribble homolog|scribbled homolog|scribbled planar cell polarity protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SCRIB, check out the SCRIB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SCRIB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human SCRIBBLE DyLight® 488 conjugated Antibody (A01651-Dyl488)
Hello CJ!
No publications found for A01651-Dyl488
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Human SCRIBBLE DyLight® 488 conjugated Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Human SCRIBBLE DyLight® 488 conjugated Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
