Anti-PARD3/Par3 Antibody

Product Name

Anti-PARD3/Par3 Antibody

View all PARD3/Par3 Antibodies

SKU/Catalog Number

A01897

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-PARD3/Par3 Antibody (Catalog # A01897). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PARD3/Par3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01897)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human PARD3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01897 is reactive to PARD3 in Human, Mouse, Rat

Reconstitution

Calculated molecular weight

151423 MW

Background of PARD3/Par3

Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions By similarity. Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.

Joberty G., Nat. Cell Biol. 2:531-539(2000).
Fang C.M., Cell Res. 11:223-229(2001).
Kohjima M., Biochem. Biophys. Res. Commun. 299:641-646(2002).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A01897 is guaranteed for IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Gene/Protein Information For PARD3 (Source: Uniprot.org, NCBI)

Alternative Names

Partitioning defective 3 homolog;PAR-3;PARD-3;Atypical PKC isotype-specific-interacting protein;ASIP;CTCL tumor antigen se2-5;PAR3-alpha;PARD3;PAR3, PAR3A; PARD3 ASIP, Baz, PAR3, PAR3alpha, PARD-3A, PPP1R118, SE2-5L16, SE2-5LT1, SE2-5T2, PARD3 par-3 family cell polarity regulator partitioning defective 3 homolog|CTCL tumor se2-5|PAR3-alpha|atypical PKC isotype-specific interacting protein|bazooka|par-3 family cell polarity regulator alpha|par-3 partitioning defective 3 homolog|protein phosphatase 1, regulatory subunit 118

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis