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Product Info Summary
| SKU: | M00602-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC-P |
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Product info
Product Name
Anti-Macrosialin CD68 Monoclonal Antibody
View all CD68/SR-D1 Antibodies
SKU/Catalog Number
M00602-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Macrosialin CD68 Monoclonal Antibody catalog # M00602-2. Tested in IF, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Macrosialin CD68 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00602-2)
Host
Mouse
Contents
PBS, pH 7.4, containing 0.5% BSA, 0.02% sodium azide and 50% Glycerol.
Clonality
Monoclonal
Clone Number
6F3
Isotype
IgG
Immunogen
Synthetic Peptide of CD68
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00602-2 is reactive to CD68 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
37408 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00602-2 is guaranteed for IF, IHC-P Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:200
IF 1:50-200
Validation Images & Assay Conditions
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IHC staining of human tonsil tissue, diluted at 1:200.
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Immunofluorescence analysis of Mouse-spleen tissue. 1, CD68 monoclonal antibody (6F3) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, CD68 monoclonal antibody (6F3) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
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Immunofluorescence analysis of Human-lung-cancer tissue. 1, CD68 monoclonal antibody (6F3) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, CD68 monoclonal antibody (6F3) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Protein Target Info & Infographic
Gene/Protein Information For CD68 (Source: Uniprot.org, NCBI)
Gene Name
CD68
Full Name
Macrosialin
Weight
37408 MW
Superfamily
LAMP family
Alternative Names
Macrosialin;Gp110;CD68;CD68; CD68 GP110, LAMP4, SCARD1 CD68 molecule macrosialin|CD68 |macrophage CD68|scavenger receptor class D, member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD68, check out the CD68 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD68: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Macrosialin CD68 Monoclonal Antibody (M00602-2)
Hello CJ!
M00602-2 has been cited in 20 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Myeloid-derived growth factor inhibits inflammation and alleviates endothelial injury and atherosclerosis in mice
Authors: Meng B,Li Y,Ding Y,Xu X,Wang L,Guo B,Zhu B,Zhang J,Xiang L,Dong J,Liu M,Xiang L,Xiang G.
Species: Mouse
M00602-2 usage in article: APP:IHC, SAMPLE:AORTIC TISSUE, DILUTION:1:200
Tauroursodeoxycholic Acid Alleviates Secondary Injury in Spinal Cord Injury Mice Through Reducing Oxidative Stress
Authors:Yonghui Hou,Jiyao Luan,Tiancheng Deng et al.
Species: Mouse
M00602-2 usage in article: APP:IF, SAMPLE:CORTICAL NEURONS, DILUTION:1:300
Schistosoma japonicum-derived peptide SJMHE1 promotes peripheral nerve repair through a macrophage-dependent mechanism
Authors:Ma Y,Wei C,Qi X,Pu Y,Dong L,Xu L,Zhou S,Zhu J,Chen X,Wang X,Su C
This study investigated whether helminths or helminth-derived molecules might have the potential to improve peripheral nerve repair. It was demonstrated that schistosome-derived SJMHE1 promoted peripheral myelin growth and functional regeneration via a macrophage-dependent mechanism and simultaneously increased the induction of M2 macrophages. The CD68 antibody from Bosterbio was used as the macrophage marker for this study. The findings indicated that schistosome-derived SJMHE1 enhances damaged peripheral nerve repair in a macrophage-dependent manner and concomitantly increases M2 macrophage polarization, highlighting the therapeutic potential of SJMHE1 for promoting peripheral nerve repair.
Species: Mouse,Rat
M00602-2 usage in article: APP:IF, SAMPLE:NERVE CONDUITS, DILUTION:1:200
Gao Y,Xu Y,Zhao S,Qian L,Song T,Zheng J,Zhang J,Chen B. Growth differentiation factor-15 promotes immune escape of ovarian cancer via targeting CD44 in dendritic cells. Exp Cell Res.2021 Mar 23:112522.doi:10.1016/j.yexcr.2021.112522.Epub ahead of print.PMID:33771482.https://pubmed.ncbi.nlm.nih.gov/33771 4 82/
Species: BA1105;APP:IF;SAMPLE:OVARIAN TISSUE;DILUTION:NA| BA1001;APP:IF;SAMPLE:OVARIAN TISSUE;DILUTION:NA|AR1176 AR1177;APP:IF;SAMPLE:OVARIAN TISSUE;DILUTION:NA|M00602-2;APP:CONFOCAL MICROSCOPY;SAMPLE:DCS;DILUTION:1:20|BA1142;APP: CONFOCAL MICROSCOPY;SAMPLE:DCS;DILUTION:NA|BA1001;APP:CONFOCAL MICROSCOPY;SAMPLE:DCS;DILUTION:NA|AR0107;APP:CO-IP;SAMPLE:DCS;DILUTION:NA|AR0105-100;APP:WB;SAMPLE:DCS; DILUTION:NA|BA1054;APP:WB;SAMPLE:DCS;DILUTION:NA
Shi D,Wu J,Wu Y,Lin X,Xu C,Lian X. High-Fat Diet-Related Obesity Promotes Urethane-Induced Lung Tumorigenesis in C57BL/6J Mice. Front Oncol.2021 Feb 23;11:620993.doi:10.3389/fonc.2021. 620993.PMID:33708630;PMCID:PMC7942226.
Species: Human,Mouse
M00602-2 usage in article: APP:IHC, SAMPLE:TRACHEA, DILUTION:1:100
High-Fat Diet-Related Obesity Promotes Urethane-Induced Lung Tumorigenesis in C57BL/6J Mice.
Authors: Shi D,Wu J,Wu Y,Lin X,Xu C,Lian X.
The study aimed to investigate whether high-fat diet (HFD)-induced obesity influences the susceptibility to chemical-induced lung tumorigenesis, a HFD feeding condition was combined with a multi-dose urethane-induced lung tumorigenesis model using C57BL/6J mice. Lung tissue slices were incubated with antibodies against CD68 from Bosterbio for the IHC assay. The findings showed that the role of HFD-induced obesity on lung tumorigenesis was elucidated both in vivo and in vitro in this study, and highly expressed leptin mediated the activation of STAT3 pathway was likely involved in the mechanism.
Species: Human,Mouse
M00602-2 usage in article: APP:IHC, SAMPLE:TRACHEA, DILUTION:1:100
Yanmin Cui,Yang Lin,Xianjun Meng,Jingxuan Ma,Haotian Deng,Xia Liu,Xinyuan He,Jin Zhao,Cyanidin-3-galactoside from Aronia melanocarpa ameliorates PM10 induced pulmonary injury by modulating M1/M2 macrophage polarization and NRF2/Sirt1 MAPK signaling,Journa
Species: Mouse
M00602-2 usage in article: APP:IHC, SAMPLE:LUNG TISSUE, DILUTION:NA
Jian Pang,Li Zhou,Liqiu Liao et al.Correlation of sTILs Infiltration and UBR5 Expression on the Efficacy of Neoadjuvant Chemotherapy Efficacy and Prognosis in Early Triple Negative Breast Cancer, 13 January 2021, PREPRINT (Version 1) available at Research
Species: Human
M00602-2 usage in article: APP:IHC, SAMPLE:BREAST TISSUE, DILUTION:NA
Fan Guo,Wei na Kong,Gang Zhao et al.The Correlation Between Tumor-associated Macrophage Infiltration and Progression in Cervical Carcinoma, 01 December 2020, PREPRINT (Version 1) available at Research Square[+https://doi.org/10.21203/rs.3.rs-115339/v1+]
Species: Human
M00602-2 usage in article: APP:IHC, SAMPLE:CERVICAL TISSUE, DILUTION:1:50
Wang,Xiaoyu & Wang,Linlin & Qi,Lili & Bian,Huimiao & Yan,Xing & Wang,Yan & Li,Xiaodong & Cho,Kenka & Wu,Guojiang & Jiang,Baohong.(2020).Protective Effects of Ginsenoside Rg1 on Acute Myocardial Infarction. Journal of Pharmacy and Pharmacology Research.04.
Species: Rat
M00602-2 usage in article: APP:IHC, SAMPLE:HEART TISSUE, DILUTION:1:100
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Customer Q&As
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1 Customer Q&As for Anti-Macrosialin CD68 Monoclonal Antibody
Question
What Are CD68 Macrophages?
Verified customer
Asked: 2021-10-20
Answer
CD68 is a protein highly expressed by cells in the monocyte lineage by circulating macrophages, and by tissue macrophages such as Kupffer cells, microglia.
Boster Scientific Support
Answered: 2021-10-20
