Click image to see more details
Product Info Summary
| SKU: | M00602 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human, Monkey, Rabbit, Cat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC |
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Product info
Product Name
Anti-CD68 (Macrophage Marker) Monoclonal Antibody
View all CD68/SR-D1 Antibodies
SKU/Catalog Number
M00602
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-CD68 (Macrophage Marker) Monoclonal Antibody (Catalog # M00602). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Monkey, Rabbit, Cat.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-CD68 (Macrophage Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00602)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: SPM130
Isotype
IgG1, kappa
Immunogen
Subcellular fraction of human alveolar macrophages
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M00602 is reactive to CD68 in Human, Monkey, Rabbit, Cat
Applications
M00602 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
37408 MW
Background of CD68/SR-D1
This antibody recognizes a glycoprotein of 110kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-CD68 Mouse Monoclonal Antibody (SPM130).
Protein Target Info & Infographic
Gene/Protein Information For CD68 (Source: Uniprot.org, NCBI)
Gene Name
CD68
Full Name
Macrosialin
Weight
37408 MW
Superfamily
LAMP family
Alternative Names
CD68 antigenmacrophage antigen CD68; CD68 molecule; CD68; DKFZp686M18236; gp110; Macrosialin; SCARD1; scavenger receptor class D, member 1; SRD1; SR-D1 CD68 GP110, LAMP4, SCARD1 CD68 molecule macrosialin|CD68 antigen|macrophage antigen CD68|scavenger receptor class D, member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD68, check out the CD68 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD68: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD68 (Macrophage Marker) Monoclonal Antibody (M00602)
Hello CJ!
M00602 has been cited in 16 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
UPLC/Q-TOFMS-Based Metabolomics Approach to Reveal the Protective Role of Other Herbs in An-Gong-Niu-Huang Wan Against the Hepatorenal Toxicity of Cinnabar and Realgar
Myeloid-derived growth factor inhibits inflammation and alleviates endothelial injury and atherosclerosis in mice
Authors: Meng B,Li Y,Ding Y,Xu X,Wang L,Guo B,Zhu B,Zhang J,Xiang L,Dong J,Liu M,Xiang L,Xiang G.
Species: Mouse
M00602 usage in article: APP:IHC, SAMPLE:AORTIC TISSUE, DILUTION:1:200
Paschalidi P,Gkouveris I,Soundia A,Kalfarentzos E,Vardas E, Georgaki M,Kostakis G,Erovic BM,Tetradis S,Perisanidis C,Nikitakis NG.The role of M1 and M2 macrophage polarization in progression of medication-related osteonecrosis of the jaw.Clin Oral Investig.2020 Sep 22:10.1007/s00784-020-03602-z.doi:10.1007/s00784-020-03602-z.Epub ahead of print.PMID:32964311;PMCID:PMC7982347.
Species: Human
M00602 usage in article: APP:IF, SAMPLE:MUCOSAL TISSUE, DILUTION:1:200
Schistosoma japonicum-derived peptide SJMHE1 promotes peripheral nerve repair through a macrophage-dependent mechanism
Authors:Ma Y,Wei C,Qi X,Pu Y,Dong L,Xu L,Zhou S,Zhu J,Chen X,Wang X,Su C
This study investigated whether helminths or helminth-derived molecules might have the potential to improve peripheral nerve repair. It was demonstrated that schistosome-derived SJMHE1 promoted peripheral myelin growth and functional regeneration via a macrophage-dependent mechanism and simultaneously increased the induction of M2 macrophages. The CD68 antibody from Bosterbio was used as the macrophage marker for this study. The findings indicated that schistosome-derived SJMHE1 enhances damaged peripheral nerve repair in a macrophage-dependent manner and concomitantly increases M2 macrophage polarization, highlighting the therapeutic potential of SJMHE1 for promoting peripheral nerve repair.
Species: Mouse,Rat
M00602 usage in article: APP:IF, SAMPLE:NERVE CONDUITS, DILUTION:1:200
Jian Pang,Li Zhou,Liqiu Liao et al.Correlation of sTILs Infiltration and UBR5 Expression on the Efficacy of Neoadjuvant Chemotherapy Efficacy and Prognosis in Early Triple Negative Breast Cancer, 13 January 2021, PREPRINT (Version 1) available at Research
Species: Human
M00602 usage in article: APP:IHC, SAMPLE:BREAST TISSUE, DILUTION:NA
Fan Guo,Wei na Kong,Gang Zhao et al.The Correlation Between Tumor-associated Macrophage Infiltration and Progression in Cervical Carcinoma, 01 December 2020, PREPRINT (Version 1) available at Research Square[+https://doi.org/10.21203/rs.3.rs-115339/v1+]
Species: Human
M00602 usage in article: APP:IHC, SAMPLE:CERVICAL TISSUE, DILUTION:1:50
Shuai Wu,Wei Yuan,Weiwei Luo et al.MiR-126 Suppresses Colitis-Associated Colorectal Cancer by Regulating Crosstalk Between Epithelium Cells and Macrophages via CXCL12, 28 December 2020, PREPRINT (Version 1) available at Research Square [https://doi.org/10
Species: Mouse,Human
M00602 usage in article: APP:IHC, SAMPLE:COLON TISSUE, DILUTION:NA
Cui M,Liang J,Xu D,Zhao L,Zhang X,Zhang L,Ren S,Liu D,Niu X,Zang YJ,Zhang B. NLRP3 inflammasome is involved in nerve recovery after sciatic nerve injury. Int Immunopharmacol.2020 Jul;84:106492.doi:10.1016/j.intimp.2020.106492.Epub 2020 May 18.PMID:3240294
Species: Mouse
M00602 usage in article: APP:IF, SAMPLE:NERVE TISSUE, DILUTION:1:200
Ye Z,Huang S,Zhang Y,Mei X,Zheng H,Li M,Chen J,Lu F.Galectins, Eosinophiles, and Macrophages May Contribute to Schistosoma japonicum Egg-Induced Immunopathology in a Mouse Model.Front Immunol.2020 Mar 13;11:146.doi:10.3389/fimmu.2020.00146.PMID:32231658;
Species: Mouse
Guo F,Feng YC,Zhao G,Zhang R,Cheng ZZ,Kong WN,Wu HL,Xu B,Lv X,Ma XM.Tumor-Associated CD163+ M2 Macrophage Infiltration is Highly Associated with PD-L1 Expression in Cervical Cancer.Cancer Manag Res.2020 Jul 15;12:5831-5843.doi:10.2147/CMAR.S257692.PMID:32
Species: Human
M00602 usage in article: APP:IHC, SAMPLE:SQUAMOUS CELLS, DILUTION:1:50
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-CD68 (Macrophage Marker) Monoclonal Antibody
Question
What Are CD68 Macrophages?
Verified customer
Asked: 2021-10-20
Answer
CD68 is a protein highly expressed by cells in the monocyte lineage by circulating macrophages, and by tissue macrophages such as Kupffer cells, microglia.
Boster Scientific Support
Answered: 2021-10-20
Question
I was wanting to use your anti-CD68 (Macrophage Marker) Monoclonal antibody for IHC for human leukocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukocyte identification?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
You can see on the product datasheet, M00602 anti-CD68 (Macrophage Marker) Monoclonal antibody has been validated for IHC on human, monkey, rabbit tissues. We have an innovator award program that if you test this antibody and show it works in human leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-30
Question
Is a blocking peptide available for product anti-CD68 (Macrophage Marker) Monoclonal antibody (M00602)?
E. Wu
Verified customer
Asked: 2020-04-20
Answer
We do provide the blocking peptide for product anti-CD68 (Macrophage Marker) Monoclonal antibody (M00602). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-20
Question
We have seen staining in human leukocyte. Are there any suggestions? Is anti-CD68 (Macrophage Marker) Monoclonal antibody supposed to stain leukocyte positively?
Verified Customer
Verified customer
Asked: 2020-02-06
Answer
Based on literature leukocyte does express CD68. Based on Uniprot.org, CD68 is expressed in leukocyte, spleen umbilical cord blood, adipose tissue, skin, platelet, liver, among other tissues. Regarding which tissues have CD68 expression, here are a few articles citing expression in various tissues:
Adipose tissue, Pubmed ID: 16625196
Liver, Pubmed ID: 19159218
Platelet, Pubmed ID: 16263699
Skin, Pubmed ID: 15489334
Spleen, and Umbilical cord blood, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-02-06
Question
See attached the WB image, lot number and protocol we used for leukocyte using anti-CD68 (Macrophage Marker) Monoclonal antibody M00602. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-29
Question
Does anti-CD68 (Macrophage Marker) Monoclonal antibody M00602 work for IHC with leukocyte?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
According to the expression profile of leukocyte, CD68 is highly expressed in leukocyte. So, it is likely that anti-CD68 (Macrophage Marker) Monoclonal antibody M00602 will work for IHC with leukocyte.
Boster Scientific Support
Answered: 2019-11-11
Question
I am looking for to test anti-CD68 (Macrophage Marker) Monoclonal antibody M00602 on human leukocyte for research purposes, then I may be interested in using anti-CD68 (Macrophage Marker) Monoclonal antibody M00602 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
The products we sell, including anti-CD68 (Macrophage Marker) Monoclonal antibody M00602, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-04
Question
I see that the anti-CD68 (Macrophage Marker) Monoclonal antibody M00602 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-16
Question
Is there a BSA free version of anti-CD68 (Macrophage Marker) Monoclonal antibody M00602 available?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD68 (Macrophage Marker) Monoclonal antibody M00602 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-01
Question
Does anti-CD68 (Macrophage Marker) Monoclonal antibody M00602 work on bovine IHC with leukocyte?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
Our lab technicians have not validated anti-CD68 (Macrophage Marker) Monoclonal antibody M00602 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-CD68 (Macrophage Marker) Monoclonal antibody M00602 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine leukocyte in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-26
Question
We are currently using anti-CD68 (Macrophage Marker) Monoclonal antibody M00602 for monkey tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, monkey, rabbit. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
The anti-CD68 (Macrophage Marker) Monoclonal antibody (M00602) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-19
Question
We were satisfied with the WB result of your anti-CD68 (Macrophage Marker) Monoclonal antibody. However we have seen positive staining in liver isoform short: cell membrane using this antibody. Is that expected? Could you tell me where is CD68 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-12-18
Answer
From what I have seen in literature, liver does express CD68. Generally CD68 expresses in isoform short: cell membrane. Regarding which tissues have CD68 expression, here are a few articles citing expression in various tissues:
Adipose tissue, Pubmed ID: 16625196
Liver, Pubmed ID: 19159218
Platelet, Pubmed ID: 16263699
Skin, Pubmed ID: 15489334
Spleen, and Umbilical cord blood, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-12-18
Question
Does M00602 anti-CD68 (Macrophage Marker) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Thomas
Verified customer
Asked: 2017-09-13
Answer
It shows on the product datasheet, M00602 anti-CD68 (Macrophage Marker) Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-09-13
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-CD68 (Macrophage Marker) Monoclonal antibody M00602. Let me know if you need anything else.
H. Dhar
Verified customer
Asked: 2017-05-18
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-18
Question
Is this M00602 anti-CD68 (Macrophage Marker) Monoclonal antibody reactive to the isotypes of CD68?
G. Jackson
Verified customer
Asked: 2015-10-15
Answer
The immunogen of M00602 anti-CD68 (Macrophage Marker) Monoclonal antibody is Subcellular fraction of human alveolar macrophages. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-10-15
Question
Can you help my question with product M00602, anti-CD68 (Macrophage Marker) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
T. Taylor
Verified customer
Asked: 2014-07-24
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00602 anti-CD68 (Macrophage Marker) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-07-24
