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Product Info Summary
| SKU: | PA2018 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Macrosialin CD68 Antibody Picoband®
View all CD68/SR-D1 Antibodies
SKU/Catalog Number
PA2018
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Macrosialin CD68 Antibody catalog # PA2018. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Macrosialin CD68 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2018)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CD68.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2018 is reactive to CD68 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
22 kDa
Calculated molecular weight
37408 MW
Background of CD68/SR-D1
CD68, cluster of differentiation, is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. CD68 is a member of a family of hematopoietic mucin-like molecules that includes leukosialin/CD43 and stem cell antigen CD34. The CD68 gene is mapped to 17p13.1. Immunohistochemistry can be used to identify the presence of CD68, which is found in the cytoplasmic granules of a range of different blood cells. It is particularly useful as a marker for the various cells of the macrophage lineage, including monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts. This allows it to be used to distinguish diseases of otherwise similar appearance, such as the monocyte/macrophage and lymphoid forms of leukaemia (the latter being CD68 negative). Its presence in macrophages also makes it useful in diagnosing conditions related to proliferation or abnormality of these cells, such as malignant histiocytosis, histiocytic lymphoma, and Gaucher's disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2018 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
IHC: Human Tonsil Tissue
Validation Images & Assay Conditions
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Anti-CD68 antibody, PA2018, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human CD68, 42.5KD
(162aa tag+ T128-L354)
Lane 1: Recombinant Human CD68 Protein 10ng
Lane 2: Recombinant Human CD68 Protein 5ng
Lane 3: Recombinant Human CD68 Protein 2.5ng
Click image to see more details
Anti-CD68 antibody, PA2018, IHC(P)
IHC(P): Human Tonsil Tissue
Protein Target Info & Infographic
Gene/Protein Information For CD68 (Source: Uniprot.org, NCBI)
Gene Name
CD68
Full Name
Macrosialin
Weight
37408 MW
Superfamily
LAMP family
Alternative Names
Macrosialin;Gp110;CD68;CD68; CD68 GP110, LAMP4, SCARD1 CD68 molecule macrosialin|CD68 |macrophage CD68|scavenger receptor class D, member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD68, check out the CD68 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD68: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Macrosialin CD68 Antibody Picoband® (PA2018)
Hello CJ!
PA2018 has been cited in 32 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Jian Pang,Li Zhou,Liqiu Liao et al.Correlation of sTILs Infiltration and UBR5 Expression on the Efficacy of Neoadjuvant Chemotherapy Efficacy and Prognosis in Early Triple Negative Breast Cancer, 13 January 2021, PREPRINT (Version 1) available at Research
Species: Human
PA2018 usage in article: APP:IHC, SAMPLE:BREAST TISSUE, DILUTION:NA
Fan Guo,Wei na Kong,Gang Zhao et al.The Correlation Between Tumor-associated Macrophage Infiltration and Progression in Cervical Carcinoma, 01 December 2020, PREPRINT (Version 1) available at Research Square[+https://doi.org/10.21203/rs.3.rs-115339/v1+]
Species: Human
PA2018 usage in article: APP:IHC, SAMPLE:CERVICAL TISSUE, DILUTION:1:50
Guo F,Feng YC,Zhao G,Zhang R,Cheng ZZ,Kong WN,Wu HL,Xu B,Lv X,Ma XM.Tumor-Associated CD163+ M2 Macrophage Infiltration is Highly Associated with PD-L1 Expression in Cervical Cancer.Cancer Manag Res.2020 Jul 15;12:5831-5843.doi:10.2147/CMAR.S257692.PMID:32
Species: Human
PA2018 usage in article: APP:IHC, SAMPLE:SQUAMOUS CELLS, DILUTION:1:50
Targeting Src attenuates peritoneal fibrosis and inhibits the epithelial to mesenchymal transition
LDL Receptor Gene-ablated Hamsters: A Rodent Model of Familial Hypercholesterolemia With Dominant Inheritance and Diet-induced Coronary Atherosclerosis
Activation of corticotropin-releasing factor receptor 1 aggravates dextran sodium sulphate-induced colitis in mice by promoting M1 macrophage polarization
Low dose doxycycline decreases systemic inflammation and improves glycemic control, lipid profiles, and islet morphology and function in db/db mice
Curcumin attenuates the development of thoracic aortic aneurysm by inhibiting VEGF expression and inflammation
Expressions Profiles of the Proteins Associated with Carbohydrate Metabolism in Rat Liver Regeneration
Inhibition of endoplasmic reticulum stress signaling pathway: A new mechanism of statins to suppress the development of abdominal aortic aneurysm
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Macrosialin CD68 Antibody Picoband®
Question
We are currently using anti-CD68 antibody PA2018 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
The anti-CD68 antibody (PA2018) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-08
Question
Do you have a BSA free version of anti-CD68 antibody PA2018 available?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD68 antibody PA2018 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-11
Question
We were happy with the WB result of your anti-CD68 antibody. However we have seen positive staining in skin isoform short: cell membrane using this antibody. Is that expected? Could you tell me where is CD68 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
Based on literature, skin does express CD68. Generally CD68 expresses in isoform short: cell membrane. Regarding which tissues have CD68 expression, here are a few articles citing expression in various tissues:
Adipose tissue, Pubmed ID: 16625196
Liver, Pubmed ID: 19159218
Platelet, Pubmed ID: 16263699
Skin, Pubmed ID: 15489334
Spleen, and Umbilical cord blood, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-10-28
Question
I see that the anti-CD68 antibody PA2018 works with IHC, what is the protocol used to produce the result images on the product page?
L. Collins
Verified customer
Asked: 2019-06-12
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-12
Question
Is this PA2018 anti-CD68 antibody reactive to the isotypes of CD68?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
The immunogen of PA2018 anti-CD68 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human CD68(341-354aa FCIIRRRPSAYQAL). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-05
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adipose tissue using anti-CD68 antibody PA2018. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-29
Question
I have a question about product PA2018, anti-CD68 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-01-15
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2018 anti-CD68 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-01-15
Question
Our lab used your anti-CD68 antibody for WB on liver a few months ago. I am using human, and I plan to use the antibody for IHC next. I am interested in examining liver as well as spleen umbilical cord blood in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2018-06-13
Answer
I looked at the website and datasheets of our anti-CD68 antibody and I see that PA2018 has been tested on human in both WB and IHC. Thus PA2018 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-06-13
Question
Is a blocking peptide available for product anti-CD68 antibody (PA2018)?
Verified Customer
Verified customer
Asked: 2018-05-29
Answer
We do provide the blocking peptide for product anti-CD68 antibody (PA2018). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-05-29
Question
Does PA2018 anti-CD68 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Edwards
Verified customer
Asked: 2017-10-26
Answer
You can see on the product datasheet, PA2018 anti-CD68 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-26
Question
My lab would like using your anti-CD68 antibody for negative regulation of dendritic cell antigen processing and presentation studies. Has this antibody been tested with western blotting on human tonsil tissue? We would like to see some validation images before ordering.
E. Dhar
Verified customer
Asked: 2016-12-26
Answer
I appreciate your inquiry. This PA2018 anti-CD68 antibody is tested on human tonsil tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-12-26
Question
We want to test anti-CD68 antibody PA2018 on human adipose tissue for research purposes, then I may be interested in using anti-CD68 antibody PA2018 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Jones
Verified customer
Asked: 2016-01-21
Answer
The products we sell, including anti-CD68 antibody PA2018, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-01-21
Question
Will anti-CD68 antibody PA2018 work for IHC with adipose tissue?
J. Parker
Verified customer
Asked: 2016-01-12
Answer
According to the expression profile of adipose tissue, CD68 is highly expressed in adipose tissue. So, it is likely that anti-CD68 antibody PA2018 will work for IHC with adipose tissue.
Boster Scientific Support
Answered: 2016-01-12
Question
See attached the WB image, lot number and protocol we used for adipose tissue using anti-CD68 antibody PA2018. Please let me know if you require anything else.
K. Walker
Verified customer
Asked: 2015-12-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-12-15
Question
I was wanting to use your anti-CD68 antibody for IHC for human adipose tissue on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human adipose tissue identification?
D. Edwards
Verified customer
Asked: 2015-01-26
Answer
It shows on the product datasheet, PA2018 anti-CD68 antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human adipose tissue in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-01-26
Question
We have seen staining in human liver. Are there any suggestions? Is anti-CD68 antibody supposed to stain liver positively?
J. Anderson
Verified customer
Asked: 2014-02-17
Answer
From literature liver does express CD68. From Uniprot.org, CD68 is expressed in leukocyte, spleen umbilical cord blood, adipose tissue, skin, platelet, liver, among other tissues. Regarding which tissues have CD68 expression, here are a few articles citing expression in various tissues:
Adipose tissue, Pubmed ID: 16625196
Liver, Pubmed ID: 19159218
Platelet, Pubmed ID: 16263699
Skin, Pubmed ID: 15489334
Spleen, and Umbilical cord blood, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2014-02-17
