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Product Info Summary
| SKU: | M01424 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IP, ICC, WB |
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Product info
Product Name
Anti-LYN Purified Monoclonal Antibody
SKU/Catalog Number
M01424
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-LYN Purified Monoclonal Antibody (Catalog# M01424). Tested in IP, WB, ICC application(s). This antibody reacts with Rat, Human, Mouse.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-LYN Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01424)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
LYN-01
Isotype
Mouse IgG1
Immunogen
Bacterially expressed recombinant fragment of human Lyn (aa 8 - 238). The antibody LYN-01 reacts with Lyn (p56/p53), a non-receptor Src-family tyrosine kinase expressed in hematopoietic tissues (intracellular antigen).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01424 is reactive to LYN in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
58574 MW
Background of LYN
Lyn is a Src-family protein tyrosine kinase that is predominantly expressed in hematopoietic cells. It is associated with a number of cell surface receptors including the B cell antigen receptor (BCR) and Fc receptors. Upon their triggering, Lyn phosphorylates subunits of these receptors in a cholesterol-dependent manner, utilizing the plasma membrane lipid raft system. The phosphorylated intracellular domains of the receptors are accessible for cytoplasmic Syk tyrosine kinase, which is activated by Lyn-mediated phosphorylation and which transduces the signal to downstream adaptors. Lyn is abnormally distributed in acute myeloid leukemia cells and seems to be a novel pharmacologic target of this disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01424 is guaranteed for IP, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1-2 μg/ml.
Validation Images & Assay Conditions
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Figure 1. Western blotting validation for Anti-LYN Purified Monoclonal Antibody M01424
Western blotting analysis (non-reducing conditions) of Lyn using anti-Lyn (LYN-01).Lane 1: negative control
Lane 2: RBL rat basophilic leukemia cell line
Lane 3: JURKAT human peripheral blood T cell leukemia cell line
Lane 4: A-431 human epidermoid carcinoma cell line
Lane 5: U-937 human histiocytic lymphoma cell line
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For LYN (Source: Uniprot.org, NCBI)
Gene Name
LYN
Full Name
Tyrosine-protein kinase Lyn
Weight
58574 MW
Superfamily
protein kinase superfamily
Alternative Names
JTK8, p56Lyn, p53Lyn LYN JTK8, p53Lyn, p56Lyn LYN proto-oncogene, Src family tyrosine kinase tyrosine-protein kinase Lyn|lck/Yes-related novel protein tyrosine kinase|v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LYN, check out the LYN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LYN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LYN Purified Monoclonal Antibody (M01424)
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No publications found for M01424
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