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Product Info Summary
| SKU: | A00789-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-TRPM7 Antibody Picoband®
SKU/Catalog Number
A00789-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRPM7 Antibody Picoband® catalog # A00789-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRPM7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00789-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human TRPM7 recombinant protein (Position: K777-K905).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00789-1 is reactive to TRPM7 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
250 kDa
Calculated molecular weight
59216 MW
Background of TRPM7
Transient receptor potential cation channel, subfamily M, member 7, also known as TRPM7, is a human gene encoding a protein of the same name. It is mapped to 15q21.2. This gene belongs to the melastatin subfamily of transient receptor potential family of ion channels. The protein encoded by this gene is both an ion channel and a serine/threonine protein kinase. The kinase activity is essential for the ion channel function, which serves to increase intracellular calcium levels and to help regulate magnesium ion homeostasis. The encoded protein is involved in cytoskeletal organization, cell adhesion, cell migration and organogenesis. Defects in this gene are a cause of amyotrophic lateral sclerosis-parkinsonism/dementia complex of Guam. The gene may also be associated with defects of cardiac function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00789-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human Hela whole cell, human COLO-320 whole cell, human 22RV1 whole cell, human SGC-7901 whole cell
ICC/IF: MCF7 cell
FCM: THP-1 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TRPM7 using anti-TRPM7 antibody (A00789-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysate,
Lane 2: human COLO-320 whole cell lysate,
Lane 3: human 22RV1 whole cell lysate,
Lane 4: human SGC-7901 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPM7 antigen affinity purified polyclonal antibody (Catalog # A00789-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPM7 at approximately 250KD. The expected band size for TRPM7 is at 212KD.
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Figure 2. IF analysis of TRPM7 using anti-TRPM7 antibody (A00789-1).
TRPM7 was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-TRPM7 Antibody (A00789-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of THP-1 cells using anti-TRPM7 antibody (A00789-1).
Overlay histogram showing THP-1 cells stained with A00789-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRPM7 Antibody (A00789-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For TRPM7 (Source: Uniprot.org, NCBI)
Gene Name
TRPM7
Full Name
Transient receptor potential cation channel subfamily M member 7
Weight
59216 MW
Alternative Names
Transient receptor potential cation channel subfamily M member 7; Channel-kinase 1; Long transient receptor potential channel 7; LTrpC-7; LTrpC7; TRPM7; CHAK1; LTRPC7 TRPM7 ALSPDC, CHAK, CHAK1, LTRPC7, LTrpC-7, TRP-PLIK transient receptor potential cation channel subfamily M member 7 transient receptor potential cation channel subfamily M member 7|LTRPC ion channel family member 7|channel-kinase 1|long transient receptor potential channel 7|transient receptor potential-phospholipase C-interacting kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRPM7, check out the TRPM7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRPM7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRPM7 Antibody Picoband® (A00789-1)
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1 Customer Q&As for Anti-TRPM7 Antibody Picoband®
Question
We are currently using anti-TRPM7 antibody A00789-1 for human tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
The anti-TRPM7 antibody (A00789-1) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-15
