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Product Info Summary
| SKU: | P01424-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Phospho-Lyn (Y396) Rabbit Monoclonal Antibody
SKU/Catalog Number
P01424-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Lyn (Y396) Rabbit Monoclonal Antibody catalog # P01424-1. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Lyn (Y396) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01424-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IFI-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-Lyn (Y396)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P01424-1 is reactive to LYN in Human, Mouse
Reconstitution
Observed Molecular Weight
130 kDa
Calculated molecular weight
58574 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01424-1 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: Jurkat cell
Validation Images & Assay Conditions
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Western blot analysis of Lyn phosphorylation expression in Jurkat cell lysate treated with Pervanadate.
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Figure 2. Flow Cytometry analysis of B cells using anti-Phospho-Lyn (Y396) rabbit monoclonal antibody (P01424-1).
Overlay histogram showing B cells stained with P01424-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 5% FBS. And then incubated with rabbit anti-Phospho-Lyn (Y396) rabbit monoclonal antibody (P01424-1) at 0.25 µl/1x106 cells 50min RT. F(ab')2-Donkey anti-Rabbit IgG (H+L), PE 0.1 µl/1x106 was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For LYN (Source: Uniprot.org, NCBI)
Gene Name
LYN
Full Name
Tyrosine-protein kinase Lyn
Weight
58574 MW
Superfamily
protein kinase superfamily
Alternative Names
Tyrosine-protein kinase Lyn;2.7.10.2;Lck/Yes-related novel protein tyrosine kinase;V-yes-1 Yamaguchi sarcoma viral related oncogene homolog;p53Lyn;p56Lyn;LYN;JTK8; LYN JTK8, p53Lyn, p56Lyn LYN proto-oncogene, Src family tyrosine kinase tyrosine-protein kinase Lyn|lck/Yes-related novel protein tyrosine kinase|v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LYN, check out the LYN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LYN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Lyn (Y396) Rabbit Monoclonal Antibody (P01424-1)
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No publications found for P01424-1
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Customer Reviews
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1 Reviews For Anti-Phospho-Lyn (Y396) Rabbit Monoclonal Antibody
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Anti-phospho Lyn (Y396) Rabbit Monoclonal Antibody
Excellent
Source: Biocompare.com
| Application | Flow Cytometry |
|---|---|
| Sample | B cells |
| Primary Incubation | 0.25 µl/10^6 cells 50min RT |
| Blocking Agent | FBS 5% |
| Secondary Incubation | F(ab')2-Donkey anti-Rabbit IgG (H+L), PE 0.1 µl/10^6 |
| Detection | Flow Cytometry |
| Results Summary | Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,25 µl/ million cells with pLYN antibody (P01424-1) during 50min After, a seconde staining was performed with 0,1µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In red secondary antibody only and in blue primary anti-phospho lyn + secondary antibody. |
Bastien Moës
Verified customer
Submitted 2024-07-16
Customer Q&As
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17 Customer Q&As for Anti-Phospho-Lyn (Y396) Rabbit Monoclonal Antibody
Question
Will P01424-1 anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
As indicated on the product datasheet, P01424-1 anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-09
Question
We are currently using anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody P01424-1 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
The anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody (P01424-1) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-27
Question
Will anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody P01424-1 work on monkey IF with liver?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
Our lab technicians have not validated anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody P01424-1 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody P01424-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey liver in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-07
Question
We are interested in using your anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody for response to amino acid studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
We appreciate your inquiry. This P01424-1 anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody is validated on jurkat cell lysate. It is guaranteed to work for Flow Cytometry, IF, ICC, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-04
Question
My boss were happy with the WB result of your anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody. However we have been able to see positive staining in brain cell membrane. nucleus. cytoplasm. using this antibody. Is that expected? Could you tell me where is LYN supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
According to literature, brain does express LYN. Generally LYN expresses in cell membrane. nucleus. cytoplasm. Regarding which tissues have LYN expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 11306681
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 9171348, 18088087
Boster Scientific Support
Answered: 2019-10-17
Question
I was wanting to use your anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody for IF for human blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human blood identification?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
It shows on the product datasheet, P01424-1 anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody has been tested for Flow Cytometry, IF, ICC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-26
Question
We have observed staining in mouse platelet. What should we do? Is anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody supposed to stain platelet positively?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
Based on literature platelet does express LYN. Based on Uniprot.org, LYN is expressed in blood, platelet, brain, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have LYN expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 11306681
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 9171348, 18088087
Boster Scientific Support
Answered: 2019-08-08
Question
Does anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody P01424-1 work for IF with blood?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
According to the expression profile of blood, LYN is highly expressed in blood. So, it is likely that anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody P01424-1 will work for IF with blood.
Boster Scientific Support
Answered: 2019-07-19
Question
Is this P01424-1 anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody reactive to the isotypes of LYN?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
The immunogen of P01424-1 anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody is A synthesized peptide derived from human Phospho-Lyn (Y396). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-11
Question
Is there a BSA free version of anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody P01424-1 available?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody P01424-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-27
Question
See below the WB image, lot number and protocol we used for blood using anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody P01424-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-01-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-16
Question
Is a blocking peptide available for product anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody (P01424-1)?
Verified Customer
Verified customer
Asked: 2018-01-25
Answer
We do provide the blocking peptide for product anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody (P01424-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-01-25
Question
you antibody to test anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody P01424-1 on human blood for research purposes, then I may be interested in using anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody P01424-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-10-26
Answer
The products we sell, including anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody P01424-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-26
Question
I see that the anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody P01424-1 works with IF, what is the protocol used to produce the result images on the product page?
A. Lewis
Verified customer
Asked: 2017-08-02
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-08-02
Question
Can you help my question with product P01424-1, anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Patel
Verified customer
Asked: 2015-08-04
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P01424-1 anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-08-04
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody P01424-1. Let me know if you need anything else.
K. Williams
Verified customer
Asked: 2015-01-16
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-01-16
Question
We have tried in the past anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody for IF on brain in a previous experiment. I am using mouse, and We intend to use the antibody for Flow Cytometry next. We are interested in examining brain as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for Flow Cytometry?
M. Gonzalez
Verified customer
Asked: 2014-08-13
Answer
I have checked the website and datasheets of our anti-Phospho-Lyn (Y396) Rabbit Monoclonal antibody and it seems that P01424-1 has been tested on mouse in both IF and Flow Cytometry. Thus P01424-1 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-08-13
