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Product Info Summary
| SKU: | PB9718 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-LOX Antibody Picoband®
SKU/Catalog Number
PB9718
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LOX Antibody Picoband® catalog # PB9718. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LOX Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9718)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human LOX, different from the related mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9718 is reactive to LOX in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
47 kDa
Calculated molecular weight
46944 MW
Background of Lox
Lysyl oxidase (LOX), also known as protein-lysine 6-oxidase, is a protein that, in humans, is encoded by the LOX gene. The protein encoded by this gene is an extracellular copper enzyme that initiates the crosslinking of collagens and elastin. The enzyme catalyzes oxidative deamination of the epsilon-amino group in certain lysine and hydroxylysine residues of collagens and lysine residues of elastin. In addition to crosslinking extracellular matrix proteins, the encoded protein may have a role in tumor suppression. Defects in this gene are a cause of autosomal recessive cutis laxa type I (CL type I). Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9718 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: rat liver tissue, rat lung tissue, mouse liver tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of LOX using anti-LOX antibody (PB9718).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX antigen affinity purified polyclonal antibody (Catalog # PB9718) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOX at approximately 47 kDa. The expected band size for LOX is at 47 kDa.
Protein Target Info & Infographic
Gene/Protein Information For LOX (Source: Uniprot.org, NCBI)
Gene Name
LOX
Full Name
Protein-lysine 6-oxidase
Weight
46944 MW
Superfamily
lysyl oxidase family
Alternative Names
Protein-lysine 6-oxidase;1.4.3.13;Lysyl oxidase;LOX; LOX AAT10 lysyl oxidase protein-lysine 6-oxidase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LOX, check out the LOX Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LOX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LOX Antibody Picoband® (PB9718)
Hello CJ!
PB9718 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Polyphenol- and Caffeine-Rich Postfermented Pu-erh Tea Improves Diet-Induced Metabolic Syndrome by Remodeling Intestinal Homeostasis in Mice
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-LOX Antibody Picoband®
Question
See attached the WB image, lot number and protocol we used for lung using anti-LOX antibody PB9718. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-10
Question
Our team were satisfied with the WB result of your anti-LOX antibody. However we have observed positive staining in skin secreted. using this antibody. Is that expected? Could you tell me where is LOX supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
From literature, skin does express LOX. Generally LOX expresses in secreted. Regarding which tissues have LOX expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1352776, 26601954
Lung, Pubmed ID: 15489334
Skin, Pubmed ID: 1357535, 7706256
Boster Scientific Support
Answered: 2020-04-09
Question
We have been able to see staining in rat smooth muscle tissue. Do you have any suggestions? Is anti-LOX antibody supposed to stain smooth muscle tissue positively?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
From what I have seen in literature smooth muscle tissue does express LOX. From what I have seen in Uniprot.org, LOX is expressed in smooth muscle tissue, skin, lung, blood, among other tissues. Regarding which tissues have LOX expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1352776, 26601954
Lung, Pubmed ID: 15489334
Skin, Pubmed ID: 1357535, 7706256
Boster Scientific Support
Answered: 2020-03-06
Question
We purchased anti-LOX antibody for WB on skin in the past. I am using human, and We want to use the antibody for ELISA next. We want examining skin as well as lung in our next experiment. Do you have any suggestion on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
I took a look at the website and datasheets of our anti-LOX antibody and it appears that PB9718 has been tested on human in both WB and ELISA. Thus PB9718 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-02-14
Question
Is this PB9718 anti-LOX antibody reactive to the isotypes of LOX?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
The immunogen of PB9718 anti-LOX antibody is A synthetic peptide corresponding to a sequence in the middle region of human LOX (240-268aa AEENCLASTAYRADVRDYDHRVLLRFPQR), different from the related mouse and rat sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-10
Question
My question regarding product PB9718, anti-LOX antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9718 anti-LOX antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-06
Question
I see that the anti-LOX antibody PB9718 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-19
Question
We are currently using anti-LOX antibody PB9718 for rat tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
H. Roberts
Verified customer
Asked: 2018-10-25
Answer
The anti-LOX antibody (PB9718) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-25
Question
Do you have a BSA free version of anti-LOX antibody PB9718 available?
Verified Customer
Verified customer
Asked: 2018-05-23
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-LOX antibody PB9718 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-05-23
Question
I am interested in to test anti-LOX antibody PB9718 on rat lung for research purposes, then I may be interested in using anti-LOX antibody PB9718 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-04-27
Answer
The products we sell, including anti-LOX antibody PB9718, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-04-27
Question
Will PB9718 anti-LOX antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
H. Jones
Verified customer
Asked: 2017-10-26
Answer
It shows on the product datasheet, PB9718 anti-LOX antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-26
Question
I was wanting to use your anti-LOX antibody for ELISA for rat lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat lung identification?
R. Johnson
Verified customer
Asked: 2017-05-09
Answer
It shows on the product datasheet, PB9718 anti-LOX antibody has been validated for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-05-09
Question
Would anti-LOX antibody PB9718 work for ELISA with lung?
R. Kulkarni
Verified customer
Asked: 2017-04-05
Answer
According to the expression profile of lung, LOX is highly expressed in lung. So, it is likely that anti-LOX antibody PB9718 will work for ELISA with lung.
Boster Scientific Support
Answered: 2017-04-05
Question
Is a blocking peptide available for product anti-LOX antibody (PB9718)?
R. Jones
Verified customer
Asked: 2015-12-09
Answer
We do provide the blocking peptide for product anti-LOX antibody (PB9718). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-12-09
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-LOX antibody PB9718. Let me know if you need anything else.
R. Johnson
Verified customer
Asked: 2014-04-29
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-04-29
