Anti-LOX Antibody Picoband®

Lox antibody

Boster Bio Anti-LOX Antibody Picoband® catalog # PB9718. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PB9718
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-LOX Antibody Picoband®

View all Lox Antibodies

SKU/Catalog Number

PB9718

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-LOX Antibody Picoband® catalog # PB9718. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-LOX Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9718)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human LOX, different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9718 is reactive to LOX in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

47 kDa

Calculated molecular weight

46944 MW

Background of Lox

Lysyl oxidase  (LOX), also known as protein-lysine 6-oxidase, is a protein that, in humans, is encoded by the LOX gene. The protein encoded by this gene is an extracellular copper enzyme that initiates the crosslinking of collagens and elastin. The enzyme catalyzes oxidative deamination of the epsilon-amino group in certain lysine and hydroxylysine residues of collagens and lysine residues of elastin. In addition to crosslinking extracellular matrix proteins, the encoded protein may have a role in tumor suppression. Defects in this gene are a cause of autosomal recessive cutis laxa type I (CL type I). Two transcript variants encoding different isoforms have been found for this gene. 

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9718 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -

Positive Control

WB: rat liver tissue, rat lung tissue, mouse liver tissue

Validation Images & Assay Conditions

Gene/Protein Information For LOX (Source: Uniprot.org, NCBI)

Gene Name

LOX

Full Name

Protein-lysine 6-oxidase

Weight

46944 MW

Superfamily

lysyl oxidase family

Alternative Names

Protein-lysine 6-oxidase;1.4.3.13;Lysyl oxidase;LOX; LOX AAT10 lysyl oxidase protein-lysine 6-oxidase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on LOX, check out the LOX Infographic

LOX infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LOX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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15 Customer Q&As for Anti-LOX Antibody Picoband®

Question

See attached the WB image, lot number and protocol we used for lung using anti-LOX antibody PB9718. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-04-10

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-10

Question

Our team were satisfied with the WB result of your anti-LOX antibody. However we have observed positive staining in skin secreted. using this antibody. Is that expected? Could you tell me where is LOX supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-04-09

Answer

From literature, skin does express LOX. Generally LOX expresses in secreted. Regarding which tissues have LOX expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1352776, 26601954
Lung, Pubmed ID: 15489334
Skin, Pubmed ID: 1357535, 7706256

Boster Scientific Support

Answered: 2020-04-09

Question

We have been able to see staining in rat smooth muscle tissue. Do you have any suggestions? Is anti-LOX antibody supposed to stain smooth muscle tissue positively?

Verified Customer

Verified customer

Asked: 2020-03-06

Answer

From what I have seen in literature smooth muscle tissue does express LOX. From what I have seen in Uniprot.org, LOX is expressed in smooth muscle tissue, skin, lung, blood, among other tissues. Regarding which tissues have LOX expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1352776, 26601954
Lung, Pubmed ID: 15489334
Skin, Pubmed ID: 1357535, 7706256

Boster Scientific Support

Answered: 2020-03-06

Question

We purchased anti-LOX antibody for WB on skin in the past. I am using human, and We want to use the antibody for ELISA next. We want examining skin as well as lung in our next experiment. Do you have any suggestion on which antibody would work the best for ELISA?

Verified Customer

Verified customer

Asked: 2020-02-14

Answer

I took a look at the website and datasheets of our anti-LOX antibody and it appears that PB9718 has been tested on human in both WB and ELISA. Thus PB9718 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-02-14

Question

Is this PB9718 anti-LOX antibody reactive to the isotypes of LOX?

Verified Customer

Verified customer

Asked: 2019-09-10

Answer

The immunogen of PB9718 anti-LOX antibody is A synthetic peptide corresponding to a sequence in the middle region of human LOX (240-268aa AEENCLASTAYRADVRDYDHRVLLRFPQR), different from the related mouse and rat sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-09-10

Question

My question regarding product PB9718, anti-LOX antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-09-06

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9718 anti-LOX antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-09-06

Question

I see that the anti-LOX antibody PB9718 works with ELISA, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-07-19

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-07-19

Question

We are currently using anti-LOX antibody PB9718 for rat tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?

H. Roberts

Verified customer

Asked: 2018-10-25

Answer

The anti-LOX antibody (PB9718) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-10-25

Question

Do you have a BSA free version of anti-LOX antibody PB9718 available?

Verified Customer

Verified customer

Asked: 2018-05-23

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-LOX antibody PB9718 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-05-23

Question

I am interested in to test anti-LOX antibody PB9718 on rat lung for research purposes, then I may be interested in using anti-LOX antibody PB9718 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-04-27

Answer

The products we sell, including anti-LOX antibody PB9718, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-04-27

Question

Will PB9718 anti-LOX antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

H. Jones

Verified customer

Asked: 2017-10-26

Answer

It shows on the product datasheet, PB9718 anti-LOX antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-10-26

Question

I was wanting to use your anti-LOX antibody for ELISA for rat lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat lung identification?

R. Johnson

Verified customer

Asked: 2017-05-09

Answer

It shows on the product datasheet, PB9718 anti-LOX antibody has been validated for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat lung in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-05-09

Question

Would anti-LOX antibody PB9718 work for ELISA with lung?

R. Kulkarni

Verified customer

Asked: 2017-04-05

Answer

According to the expression profile of lung, LOX is highly expressed in lung. So, it is likely that anti-LOX antibody PB9718 will work for ELISA with lung.

Boster Scientific Support

Answered: 2017-04-05

Question

Is a blocking peptide available for product anti-LOX antibody (PB9718)?

R. Jones

Verified customer

Asked: 2015-12-09

Answer

We do provide the blocking peptide for product anti-LOX antibody (PB9718). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-12-09

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-LOX antibody PB9718. Let me know if you need anything else.

R. Johnson

Verified customer

Asked: 2014-04-29

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-04-29

Order DetailsPrice
PB9718

100μg

$370
PB9718-10ug

10μg sample (liquid)

$99
PB9718-Biotin

100 μg Biotin conjugated

$570
PB9718-Cy3

100 μg Cy3 conjugated

$570
PB9718-Dylight488

100 μg Dylight488 conjugated

$570
PB9718-Dylight550

100 μg Dylight550 conjugated

$570
PB9718-Dylight594

100 μg Dylight594 conjugated

$570
PB9718-FITC

100 μg FITC conjugated

$570
PB9718-HRP

100 μg HRP conjugated

$570
PB9718-APC

100 μg APC conjugated

$670
PB9718-PE

100 μg PE conjugated

$670
PB9718-iFluor647

100 μg iFluor647 conjugated

$670
PB9718-carrier-free

Carrier Free

$370
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PB9718
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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