Product Info Summary
SKU: | RP1031 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-5 Lipoxygenase/ALOX5 Antibody
View all 5-Lipoxygenase Antibodies
SKU/Catalog Number
RP1031
PB0008 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-5 Lipoxygenase/ALOX5 Antibody catalog # RP1031. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-5 Lipoxygenase/ALOX5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1031)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ALOX5 recombinant protein (Position: A120-R483). Human ALOX5 shares 94% amino acid (aa) sequence identity with both mouse and rat ALOX5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1031 is reactive to ALOX5 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
78 kDa
Calculated molecular weight
77983 MW
Background of 5-Lipoxygenase
Arachidonate 5-lipoxygenase, also known as 5-LOX or 5-LO, is an enzyme that in humans is encoded by the ALOX5 gene. ALOX5 is a member of the lipoxygenase family of enzymes. It is mapped to 10q11.21. ALOX5 plays a dual role in the synthesis of leukotrienes from arachidonic acid. The position of ALOX5 within the nucleus of resting cells determines the capacity to generate LTB4 upon subsequent activation. It is involved in lung vascular tone regulation and in the development of chronic pulmonary hypertension in hypoxic rodent models. ALOX5 also transforms EFAs into leukotrienes and is a current target for pharmaceutical intervention in a number of diseases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1031 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: SGC Whole Cell
IHC: Mouse Brain Tissue, Rat Brain Tissue, Human Intestinal Cancer Tissue, Human Mammary Cancer Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-ALOX5 Picoband antibody, RP1031-1.JPG
IHC(P): Mouse Brain Tissue
Click image to see more details
Anti-ALOX5 Picoband antibody, RP1031-2.JPG
IHC(P): Rat Brain Tissue
Click image to see more details
Anti-ALOX5 Picoband antibody, RP1031-3.JPG
IHC(P): Human Intestinal Cancer Tissue
Click image to see more details
Anti-ALOX5 Picoband antibody, RP1031-4.JPG
IHC(P): Human Mammary Cancer Tissue
Click image to see more details
Anti-ALOX5 Picoband antibody, RP1031-5.jpg
All lanes: Anti-ALOX5 (RP1031) at 0.5ug/ml
WB: SGC Whole Cell Lysate at 40ug
Predicted bind size: 78KD
Observed bind size: 78KD
Protein Target Info & Infographic
Gene/Protein Information For ALOX5 (Source: Uniprot.org, NCBI)
Gene Name
ALOX5
Full Name
Arachidonate 5-lipoxygenase
Weight
77983 MW
Superfamily
lipoxygenase family
Alternative Names
Arachidonate 5-lipoxygenase;5-LO;5-lipoxygenase;1.13.11.34;ALOX5;LOG5; ALOX5 5-LO, 5-LOX, 5LPG, LOG5 arachidonate 5-lipoxygenase polyunsaturated fatty acid 5-lipoxygenase|LOX-5|arachidonic acid 5-lipoxygenase|leukotriene A4 synthase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ALOX5, check out the ALOX5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ALOX5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-5 Lipoxygenase/ALOX5 Antibody (RP1031)
Hello CJ!
No publications found for RP1031
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-5 Lipoxygenase/ALOX5 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-5 Lipoxygenase/ALOX5 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-5 Lipoxygenase/ALOX5 Antibody
Question
We are currently using anti-5 Lipoxygenase/ALOX5 antibody RP1031 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
The anti-5 Lipoxygenase/ALOX5 antibody (RP1031) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-06