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Product Info Summary
| SKU: | A04876-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-POMT2 Antibody Picoband®
SKU/Catalog Number
A04876-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-POMT2 Antibody Picoband® catalog # A04876-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-POMT2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04876-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human POMT2, which shares 61.9% amino acid (aa) sequence identity with both mouse and rat POMT2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04876-1 is reactive to POMT2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
84 kDa
Calculated molecular weight
35883 MW
Background of POMT2
Protein O-mannosyl-transferase 2 is an enzyme that in humans is encoded by the POMT2 gene. This gene is mapped to chromosome 14q24.3. The protein encoded by this gene is an O-mannosyltransferase that requires interaction with the product of the POMT1 gene for enzymatic function. The encoded protein is found in the membrane of the endoplasmic reticulum. Defects in this gene are a cause of Walker-Warburg syndrome (WWS).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04876-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Positive Control
WB: human PC-3 whole cell, human Caco-2 whole cell, human HepG2 whole cell, rat lung tissue, rat testis tissue, rat spleen tissue, mouse lung tissue, mouse testis tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of POMT2 using anti-POMT2 antibody (A04876-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: rat lung tissue lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat spleen tissue lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POMT2 antigen affinity purified polyclonal antibody (Catalog # A04876-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POMT2 at approximately 84KD. The expected band size for POMT2 is at 84KD.
Protein Target Info & Infographic
Gene/Protein Information For POMT2 (Source: Uniprot.org, NCBI)
Gene Name
POMT2
Full Name
Protein O-mannosyl-transferase 2
Weight
35883 MW
Superfamily
glycosyltransferase 39 family
Alternative Names
Protein O-mannosyl-transferase 2; Dolichyl-phosphate-mannose--protein mannosyltransferase 2; POMT2 POMT2 LGMD2N, LGMDR14, MDDGA2, MDDGB2, MDDGC2 protein O-mannosyltransferase 2 protein O-mannosyl-transferase 2|dolichyl-phosphate-mannose--protein mannosyltransferase 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POMT2, check out the POMT2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POMT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-POMT2 Antibody Picoband® (A04876-1)
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Customer Q&As
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13 Customer Q&As for Anti-POMT2 Antibody Picoband®
Question
I have a question about product A04876-1, anti-POMT2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04876-1 anti-POMT2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-29
Question
Is a blocking peptide available for product anti-POMT2 antibody (A04876-1)?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
We do provide the blocking peptide for product anti-POMT2 antibody (A04876-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-07
Question
Would A04876-1 anti-POMT2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
As indicated on the product datasheet, A04876-1 anti-POMT2 antibody as been tested on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-28
Question
Will anti-POMT2 antibody A04876-1 work on pig IHC-P with neuroblastoma?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
Our lab technicians have not validated anti-POMT2 antibody A04876-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-POMT2 antibody A04876-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig neuroblastoma in IHC-P, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-24
Question
Would anti-POMT2 antibody A04876-1 work for IHC-P with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
According to the expression profile of cervix carcinoma erythroleukemia, POMT2 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-POMT2 antibody A04876-1 will work for IHC-P with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2019-08-05
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-POMT2 antibody A04876-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-21
Question
Is this A04876-1 anti-POMT2 antibody reactive to the isotypes of POMT2?
M. Li
Verified customer
Asked: 2019-05-09
Answer
The immunogen of A04876-1 anti-POMT2 antibody is A synthetic peptide corresponding to a sequence of human POMT2 (QAARAAGRDVAAEAVARSPKR). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-09
Question
I am looking for to test anti-POMT2 antibody A04876-1 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-POMT2 antibody A04876-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-01-31
Answer
The products we sell, including anti-POMT2 antibody A04876-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-01-31
Question
I was wanting to use your anti-POMT2 antibody for IHC-P for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2018-10-22
Answer
As indicated on the product datasheet, A04876-1 anti-POMT2 antibody has been validated for IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-22
Question
Is there a BSA free version of anti-POMT2 antibody A04876-1 available?
Verified Customer
Verified customer
Asked: 2018-10-01
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-POMT2 antibody A04876-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-10-01
Question
Here is the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-POMT2 antibody A04876-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-07-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-05
Question
We are currently using anti-POMT2 antibody A04876-1 for mouse tissue, and we are happy with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-21
Answer
The anti-POMT2 antibody (A04876-1) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-21
Question
I see that the anti-POMT2 antibody A04876-1 works with IHC-P, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-11-14
Answer
You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-11-14
