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Product Info Summary
| SKU: | EK0533 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human TRAILR3/TNFRSF10C ELISA Kit PicoKine®
View all TRAILR3/TNFRSF10C ELISA kits
SKU/Catalog Number
EK0533
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TRAILR3/TNFRSF10C ELISA Kit PicoKine® (96 Tests). Quantitate Human TNFRSF10C in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TRAILR3/TNFRSF10C ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0533)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A26-A236
Sensitivity
<12 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0533 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0533 is reactive to TNFRSF10C in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK0533 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TRAILR3/TNFRSF10C
TRAILR3/TNFRSF10C is a member of the TNF-receptor superfamily. This receptor contains an extracellular TRAIL-binding domain and a transmembrane domain, but no cytoplasmic death domain. This receptor is not capable of inducing apoptosis, and is thought to function as an antagonistic receptor that protects cells from TRAIL-induced apoptosis. TRAILR3 gene was found to be a p53-regulated DNA damage-inducible gene. The expression of this gene was detected in many normal tissues but not in most cancer cell lines, which may explain the specific sensitivity of cancer cells to the apoptosis-inducing activity of TRAIL.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0533-CAP | Anti-Human TNFRSF10C Pre-coated 96-well strip microplate | 1 |
| EK0533-ST | Human TNFRSF10C Standard | 2 vials, 10 ng/tube |
| EK0533-DA | Human TNFRSF10C Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 | |
| O.D. | 0.051 | 0.116 | 0.184 | 0.322 | 0.612 | 1.103 | 1.738 | 2.388 |
Data Example Images
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Human TRAILR3/ TNFRSF10C PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TRAILR3/TNFRSF10C, Dilution ratio of 1:1, concentration in serum and plasma is around 1 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TRAILR3/TNFRSF10C ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 154 | 584 | 1628 | 145 | 568 | 1473 |
| Standard deviation | 8.31 | 40.88 | 109.07 | 9.42 | 46 | 103.11 |
| CV (%) | 5.4% | 7% | 6.7% | 6.5% | 8.1% | 7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TRAILR3/TNFRSF10C in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 154 | 163 | 147 | 142 | 151 | 7.88 | 5.2% |
| Sample 2 | 584 | 548 | 550 | 549 | 557 | 15.17 | 2.7% |
| Sample 3 | 1628 | 1534 | 1759 | 1601 | 1630 | 81.7 | 5% |
Protein Target Info & Infographic
Gene/Protein Information For TNFRSF10C (Source: Uniprot.Org, NCBI)
Gene Name
TNFRSF10C
Full Name
Tumor necrosis factor receptor superfamily member 10C
Weight
27.407kDa
Alternative Names
Tumor necrosis factor receptor superfamily member 10C; Antagonist decoy receptor for TRAIL/Apo-2L; Decoy TRAIL receptor without death domain; Decoy receptor 1; DcR1; Lymphocyte inhibitor of TRAIL; TNF-related apoptosis-inducing ligand receptor 3; TRAIL receptor 3; TRAIL-R3; TRAIL receptor without an intracellular domain; CD263; TNFRSF10C; DCR1, LIT, TRAILR3, TRID; UNQ321/PRO366; TNFRSF10C CD263, DCR1, DCR1-TNFR, LIT, TRAIL-R3, TRAILR3, TRID TNF receptor superfamily member 10c tumor necrosis factor receptor superfamily member 10C|TNF-related apoptosis-inducing ligand receptor 3|antagonist decoy receptor for TRAIL/Apo-2L|cytotoxic TRAIL receptor-3|decoy TRAIL receptor without death domain|decoy receptor 1|lymphocyte inhibitor of TRAIL|tumor necrosis factor receptor superfamily, member 10c, decoy without an intracellular domain
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TNFRSF10C, check out the TNFRSF10C Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TNFRSF10C: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human TRAILR3/TNFRSF10C ELISA Kit PicoKine® (EK0533)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Human TRAILR3/TNFRSF10C ELISA Kit PicoKine®
Question
Q: can I use citrate plasma as samples in Human TRAILR3 Picokine® ELISA Kit (Catalog # EK0533)?
Verified Customer
Verified customer
Asked: 2020-09-27
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of TRAILR3 causing disruption of its protein structure. TRAILR3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the TRAILR3 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-09-27
Question
Q: Can TRAILR3 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-02-24
Question
Q: how to analyze ELISA data? I measured TRAILR3 level in plasma.
D. Adams
Verified customer
Asked: 2018-12-15
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-12-15
Question
Q: What is the optimal O.D. value for TRAILR3 ELISA kit? I used your TRAILR3 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain TRAILR3 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-11-14
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TRAILR3 ELISA kit to have sensitivity of 12pg/ml, that means the minimum amount of TRAILR3 that can be declared/interpreted as positive by the above standard is 12pg/ml.
Boster Scientific Support
Answered: 2018-11-14
Question
Q: if the enzyme conjugated TRAILR3 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TRAILR3 antigen?
Verified Customer
Verified customer
Asked: 2018-10-22
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-10-22
Question
Q: how can I thaw whole blood sample for TRAILR3 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-04-18
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TRAILR3 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-04-18
