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Product Info Summary
| SKU: | A11875 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB |
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Product info
Product Name
Anti-Kremen protein 2 KREMEN2 Antibody
SKU/Catalog Number
A11875
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Kremen protein 2 KREMEN2 Antibody (Catalog # A11875). Tested in ELISA, WB, ICC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
KREMEN2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Kremen protein 2 KREMEN2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11875)
Host
Rabbit
Contents
KREMEN2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-KREMEN2 antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human KREMEN2. The immunogen is located within amino acids 390-440 of KREMEN2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Multiple isoforms of KREMEN2 are known to exist. KREMEN2 antibody is predicted to not cross-react with other Kremen protein family members.
Reactive Species
A11875 is reactive to KREMEN2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
48849 MW
Background of Kremen-2
Kremen (Kringle containing protein marking the eye and the nose) proteins are type I transmembrane proteins that contain extracellular kringle, WSC and CUB domains and an intracellular region without any conserved motifs. Kremens bind a subset of the secreted Dickkopf proteins (Dkk 1, 2, and 4) with high affinity to modulate the canonical Wnt signaling pathway that is transduced by the ternary receptor complex composed of Wnt, Frizzled, and the LDL receptor related protein 5/6 (LRP5/6) co-receptor. Recent experiments have shown that KREMEN2 is a regulator of bone remodeling and raise the possibility that antagonizing KREMEN2 might prove beneficial in patients with bone loss disorders.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11875 is guaranteed for ELISA, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1-2 μg/mL; ICC: 5 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: KREMEN2, A11875 (2 μg/mL), KREMEN2, (1 μg/mL) and beta-actin, (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Human Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: KREMEN2, A11875 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Mouse Spleen Tissue Lysate
Loading: 15 μg of lysates per lane. Antibodies: KREMEN2 A11875 (Lane 1: 1 μg/mL and Lane 2: 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunocytochemistry Validation of KREMEN2 in HeLa Cells
Immunocytochemical analysis of HeLa cells using anti-KREMEN2 antibody (A11875) at 5 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Protein Target Info & Infographic
Gene/Protein Information For KREMEN2 (Source: Uniprot.org, NCBI)
Gene Name
KREMEN2
Full Name
Kremen protein 2
Weight
48849 MW
Alternative Names
KRM2, KRM2, Kremen protein 2, Dickkopf receptor 2 Kremen2|2900054E04Rik, Krm, Krm2|kringle containing transmembrane protein 2|kremen protein 2|dickkopf receptor 2|kringle domain-containing transmembrane protein 2|kringle-containing protein marking the eye and the nose
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KREMEN2, check out the KREMEN2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KREMEN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Kremen protein 2 KREMEN2 Antibody (A11875)
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