Product Info Summary
SKU: | A08108 |
---|---|
Size: | 500ug |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-RNF34 Antibody
SKU/Catalog Number
A08108
Size
500ug
Form
Lyophilized
Description
Boster Bio Anti-RNF34 Antibody (Catalog # A08108). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-RNF34 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08108)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-373 of human RNF34 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
beta-Actin antibody is human, mouse, rat, rabbit, chicken, zebrafish and drosophila reactive.
Reactive Species
A08108 is reactive to RNF34 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 500 µL
Observed Molecular Weight
68 kDa
Calculated molecular weight
38450 MW
Background of RNF34
RNF34, also known as RING finger protein 34, RING finger protein RIFF, FYVE-RING finger protein Momo, Human RING finger homologous to inhibitor of apoptosis protein, hRFI, Caspases-8 and -10-associated RING finger protein 1, CARP-1 and Caspase regulator CARP1, is a novel modulator of NF-kB activation. RNF34 possesses E3 ubiquitin protein ligase activity and has been shown to directly and indirectly regulate the levels of p53/TP53 through ubiquitination, targeting to proteasomal degradation and degradation of SFN. Anti-RNF34 Antibody is useful for researcher interested in Immunology, p53 regulation, Transcription, and Nuclear Signaling research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08108 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:4,000 - 1:20,000
IF Microscopy: User optimized
WB: 1:500 - 1:3,000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of RNF34 using anti-RNF34 antibody (A08108).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF34 antigen affinity purified polyclonal antibody (Catalog # A08108) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for RNF34.
Protein Target Info & Infographic
Gene/Protein Information For RNF34 (Source: Uniprot.org, NCBI)
Gene Name
RNF34
Full Name
E3 ubiquitin-protein ligase RNF34
Weight
38450 MW
Alternative Names
RNF34, RNF 34, Caspase regulator CARP1, CARP 1, hRFI, RFI, RIFF, Ring finger protein 34 RNF34 CARP-1, CARP1, RFI, RIF, RIFF, hRFI ring finger protein 34 E3 ubiquitin-protein ligase RNF34|FYVE-RING finger protein MOMO|RING finger protein RIFF|RING-type E3 ubiquitin transferase RNF34|caspase regulator CARP1|caspases-8 and -10-associated RING finger protein 1|human RING finger homologous to inhibitor of apoptosis protein|ring finger protein 34, E3 ubiquitin protein ligase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RNF34, check out the RNF34 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RNF34: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RNF34 Antibody (A08108)
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Customer Q&As
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6 Customer Q&As for Anti-RNF34 Antibody
Question
Will A08108 anti-RNF34 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
It shows on the product datasheet, A08108 anti-RNF34 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-24
Question
Would anti-RNF34 antibody A08108 work on primate IF with trachea?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
Our lab technicians have not validated anti-RNF34 antibody A08108 on primate. You can run a BLAST between primate and the immunogen sequence of anti-RNF34 antibody A08108 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate trachea in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-02
Question
My question regarding product A08108, anti-RNF34 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08108 anti-RNF34 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-15
Question
Does anti-RNF34 antibody A08108 work for WB with leukemic t-cell?
T. Parker
Verified customer
Asked: 2018-01-18
Answer
According to the expression profile of leukemic t-cell, RNF34 is highly expressed in leukemic t-cell. So, it is likely that anti-RNF34 antibody A08108 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2018-01-18
Question
We are currently using anti-RNF34 antibody A08108 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
S. Carter
Verified customer
Asked: 2016-10-28
Answer
The anti-RNF34 antibody (A08108) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-10-28
Question
I was wanting to use your anti-RNF34 antibody for WB for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?
B. Carter
Verified customer
Asked: 2016-06-08
Answer
As indicated on the product datasheet, A08108 anti-RNF34 antibody has been tested for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-06-08