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Product Info Summary
| SKU: | PA1638 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Kallistatin/SERPINA4 Antibody Picoband®
View all Serpin A4/Kallistatin Antibodies
SKU/Catalog Number
PA1638
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Kallistatin/SERPINA4 Antibody catalog # PA1638. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Kallistatin/SERPINA4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1638)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Kallistatin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1638 is reactive to SERPINA4 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
91 kDa
Calculated molecular weight
48542 MW
Background of Serpin A4/Kallistatin
Serpina 4, also called Kallistatin, PI4 or TISSUE KALLIKREIN INHIBITOR, is a protein that in humans is encoded by the SERPINA4 gene. The serpina4 is a member of the serpin superfamily and represents a major inhibitor of human tissue kallikrein in the circulation. The SERPINA4 gene is localized to 14q32.13. The translated amino acid sequence of serpina4 matches the protein sequence and shares 44 to 46% sequence identity with other serpin family members. Kallistatin is a serine proteinase inhibitor (serpin) and a heparin-binding protein. It is localized in vascular smooth muscle cells and endothelial cells of blood vessels, suggesting that it may be involved in the regulation of vascular function. And serpina4 also plays a role in neointima hyperplasia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1638 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: SMMC Cell, COLO320 Cell, JURKAT Cell,, HELA Cell, SKOV Cell,
IHC: Human Liver Cancer Tissue
Validation Images & Assay Conditions
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Anti-Kallistatin antibody, PA1638, IHC(P)
IHC(P): Human Liver Cancer Tissue
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Anti-Kallistatin antibody, PA1638, Western blotting
Lane 1: SMMC Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: JURKAT Cell Lysate
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Anti-Kallistatin antibody, PA1638, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: SKOV Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For SERPINA4 (Source: Uniprot.org, NCBI)
Gene Name
SERPINA4
Full Name
Kallistatin
Weight
48542 MW
Superfamily
serpin family
Alternative Names
Kallistatin;Kallikrein inhibitor;Peptidase inhibitor 4;PI-4;Serpin A4;SERPINA4;KST, PI4; SERPINA4 KAL, KLST, KST, PI-4, PI4, kallistatin serpin family A member 4 kallistatin|kallikrein inhibitor|peptidase inhibitor 4|protease inhibitor 4 (kallistatin)|serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4|serpin A4|serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4|sserpin family A member 4|testicular secretory protein Li 22
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SERPINA4, check out the SERPINA4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SERPINA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Kallistatin/SERPINA4 Antibody Picoband® (PA1638)
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Customer Q&As
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3 Customer Q&As for Anti-Kallistatin/SERPINA4 Antibody Picoband®
Question
I see that the anti-Kallistatin/SERPINA4 antibody PA1638 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-24
Question
Would anti-Kallistatin/SERPINA4 antibody PA1638 work on feline IHC with colon?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
Our lab technicians have not validated anti-Kallistatin/SERPINA4 antibody PA1638 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Kallistatin/SERPINA4 antibody PA1638 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline colon in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-10
Question
We are currently using anti-Kallistatin/SERPINA4 antibody PA1638 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-02
Answer
The anti-Kallistatin/SERPINA4 antibody (PA1638) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-02
