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Product Info Summary
| SKU: | PA1632 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-TIM 1/HAVCR1 Antibody Picoband®
View all TIM-1/KIM-1/HAVCR Antibodies
SKU/Catalog Number
PA1632
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TIM 1/HAVCR1 Antibody catalog # PA1632. Tested in IHC, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TIM 1/HAVCR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1632)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of rat TIM 1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1632 is reactive to Havcr1 in Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50 kDa
Calculated molecular weight
33964 MW
Background of TIM-1/KIM-1/HAVCR
KIM1 (KIDNEY INJURY MOLECULE 1), also known as HAVCR1, HAVCR or TIM1, is a protein that in humans is encoded by the KIM1 gene. The KIM1 gene is mapped to 5q33.3. Biochemical, mutational, and cell adhesion analyses confirm that Tim1 is capable of homophilic Tim-Tim interactions. The features identified in murine KIM1 is conserved in human KIM1. The KIM1 protein is indeed a receptor for the virus through the infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV. Using a monoclonal antibody to mouse Tim1, Tim1 is expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. Ectopic expression of KIM1 during mouse T-cell differentiation leads to production of the Th2-type cytokine Il4, but not the Th1-type cytokine Ifng. KIM1-expressing epithelial cells internalized apoptotic bodies, and Kim1 is directly responsible for phagocytosis in cultured primary rat tubule epithelial cells and in porcine and canine epithelial cell lines.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1632 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Rat
Positive Control
WB: rat kidney tissue, rat testis tissue, rat heart tissue
IHC: rat testis tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of KIM1 using anti-KIM1 antibody (PA1632).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: rat testis tissue lysates,
Lane 3: rat heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIM1 antigen affinity purified polyclonal antibody (Catalog # PA1632) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIM1 at approximately 50KD. The expected band size for KIM1 is at 39KD.
Click image to see more details
Figure 2. IHC analysis of KIM1 using anti-KIM1 antibody (PA1632).
KIM1 was detected in paraffin-embedded section of rat testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KIM1 Antibody (PA1632) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Havcr1 (Source: Uniprot.org, NCBI)
Gene Name
Havcr1
Full Name
Hepatitis A virus cellular receptor 1
Weight
33964 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Hepatitis A virus cellular receptor 1 homolog;HAVcr-1;Kidney injury molecule 1;KIM-1;T cell immunoglobulin and mucin domain-containing protein 1;TIMD-1;Havcr1;Kim1; HAVCR1 CD365, HAVCR, HAVCR-1, KIM-1, KIM1, TIM, TIM-1, TIM1, TIMD-1, TIMD1 hepatitis A virus cellular receptor 1 hepatitis A virus cellular receptor 1|T cell immunoglobin domain and mucin domain protein 1|T-cell immunoglobulin mucin family member 1|T-cell immunoglobulin mucin receptor 1|T-cell membrane protein 1|kidney injury molecule 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Havcr1, check out the Havcr1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Havcr1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TIM 1/HAVCR1 Antibody Picoband® (PA1632)
Hello CJ!
PA1632 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Resveratrol ameliorates sepsis‑induced acute kidney injury in a pediatric rat model via Nrf2 signaling pathway
Sánchez-Navarro A,Pérez-Villalva R,Murillo-de-Ozores AR,Martínez-Rojas MÁ,Rodríguez-Aguilera JR,González N,Castañeda-Bueno M,Gamba G,Recillas-Targa F,Bobadilla NA.Vegfa promoter gene hypermethylation at HIF1α binding site is an early contributor to CKD progression after renal ischemia.Sci Rep.2021 Apr 22;11(1):8769.doi:10.1038/s41598-021-88000-5.PMID:33888767;PMCID:PMC8062449.
Species: Rat
PA1632 usage in article: APP:WB, SAMPLE:URINE, DILUTION: 1:5000
Resveratrol ameliorates sepsis%u2011induced acute kidney injury in a pediatric rat model via Nrf2 signaling pathway
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-TIM 1/HAVCR1 Antibody Picoband®
Question
Our lab were happy with the WB result of your anti-TIM 1/HAVCR1 antibody. However we have seen positive staining in adult mammalian kidney membrane using this antibody. Is that expected? Could you tell me where is HAVCR1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
Based on literature, adult mammalian kidney does express HAVCR1. Generally HAVCR1 expresses in membrane. Regarding which tissues have HAVCR1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 9658108, 15372022
Boster Scientific Support
Answered: 2020-05-06
Question
We are currently using anti-TIM 1/HAVCR1 antibody PA1632 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
The anti-TIM 1/HAVCR1 antibody (PA1632) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-16
Question
Can you help my question with product PA1632, anti-TIM 1/HAVCR1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1632 anti-TIM 1/HAVCR1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-17
Question
We have been able to see staining in rat kidney. What should we do? Is anti-TIM 1/HAVCR1 antibody supposed to stain kidney positively?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
From what I have seen in literature kidney does express HAVCR1. From what I have seen in Uniprot.org, HAVCR1 is expressed in adult mammalian kidney, liver, kidney, cervix carcinoma, among other tissues. Regarding which tissues have HAVCR1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 9658108, 15372022
Boster Scientific Support
Answered: 2019-10-11
Question
I was wanting to use your anti-TIM 1/HAVCR1 antibody for WB for rat kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat kidney identification?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
You can see on the product datasheet, PA1632 anti-TIM 1/HAVCR1 antibody has been validated for IHC, WB on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-04
Question
Does PA1632 anti-TIM 1/HAVCR1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-04-19
Answer
As indicated on the product datasheet, PA1632 anti-TIM 1/HAVCR1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-19
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-TIM 1/HAVCR1 antibody PA1632. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-04-18
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-18
Question
Is this PA1632 anti-TIM 1/HAVCR1 antibody reactive to the isotypes of HAVCR1?
Verified Customer
Verified customer
Asked: 2019-02-14
Answer
The immunogen of PA1632 anti-TIM 1/HAVCR1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of rat TIM 1(289-307aa HPRAEDNIYIIEDRSRGAE). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-14
Question
I was wanting to use using your anti-TIM 1/HAVCR1 antibody for response to testosterone studies. Has this antibody been tested with western blotting on rat heart tissue? We would like to see some validation images before ordering.
S. Moore
Verified customer
Asked: 2018-08-17
Answer
I appreciate your inquiry. This PA1632 anti-TIM 1/HAVCR1 antibody is tested on rat heart tissue, kidney tissue, testis tissue. It is guaranteed to work for IHC, WB in rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-08-17
Question
I see that the anti-TIM 1/HAVCR1 antibody PA1632 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-08-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-08-07
Question
Does anti-TIM 1/HAVCR1 antibody PA1632 work on feline IHC with adult mammalian kidney?
B. Baker
Verified customer
Asked: 2018-05-30
Answer
Our lab technicians have not validated anti-TIM 1/HAVCR1 antibody PA1632 on feline. You can run a BLAST between feline and the immunogen sequence of anti-TIM 1/HAVCR1 antibody PA1632 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline adult mammalian kidney in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-30
Question
See below the WB image, lot number and protocol we used for kidney using anti-TIM 1/HAVCR1 antibody PA1632. Please let me know if you require anything else.
K. Jha
Verified customer
Asked: 2018-01-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-19
Question
Is there a BSA free version of anti-TIM 1/HAVCR1 antibody PA1632 available?
Verified Customer
Verified customer
Asked: 2017-09-21
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TIM 1/HAVCR1 antibody PA1632 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-09-21
Question
I would like to test anti-TIM 1/HAVCR1 antibody PA1632 on rat kidney for research purposes, then I may be interested in using anti-TIM 1/HAVCR1 antibody PA1632 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Johnson
Verified customer
Asked: 2017-01-05
Answer
The products we sell, including anti-TIM 1/HAVCR1 antibody PA1632, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-01-05
Question
Is a blocking peptide available for product anti-TIM 1/HAVCR1 antibody (PA1632)?
K. Krishna
Verified customer
Asked: 2016-12-21
Answer
We do provide the blocking peptide for product anti-TIM 1/HAVCR1 antibody (PA1632). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-12-21
Question
We bought anti-TIM 1/HAVCR1 antibody for IHC on kidney last year. I am using rat, and We want to use the antibody for WB next. I am looking for examining kidney as well as adult mammalian kidney in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
J. Jha
Verified customer
Asked: 2015-08-10
Answer
I took a look at the website and datasheets of our anti-TIM 1/HAVCR1 antibody and I see that PA1632 has been tested on rat in both IHC and WB. Thus PA1632 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-08-10
Question
Does anti-TIM 1/HAVCR1 antibody PA1632 work for WB with kidney?
M. Kulkarni
Verified customer
Asked: 2013-08-13
Answer
According to the expression profile of kidney, HAVCR1 is highly expressed in kidney. So, it is likely that anti-TIM 1/HAVCR1 antibody PA1632 will work for WB with kidney.
Boster Scientific Support
Answered: 2013-08-13
