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Product Info Summary
| SKU: | A02068-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-JAM-A/F11R Antibody Picoband®
SKU/Catalog Number
A02068-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-JAM-A/F11R Antibody Picoband® catalog # A02068-2. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-JAM-A/F11R Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02068-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human JAM-A/F11R, which shares 75% and 80% amino acid (aa) sequence identity with mouse and rat JAM-A/F11R, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02068-2 is reactive to F11R in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
41 kDa
Calculated molecular weight
32.424kDa
Background of JAM-A
Junctional adhesion molecule A is a protein that in humans is encoded by the F11R gene. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02068-2 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human placenta tissue, human CACO-2 whole cell, human MDA-MB-453 whole cell, human HEPG2 whole cell
ICC/IF: MCF7 cell
FCM: HEPG2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of JAM-A/F11R using anti-JAM-A/F11R antibody (A02068-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human CACO-2 whole cell lysates,
Lane 3: human MDA-MB-453 whole cell lysates,
Lane 4: human HEPG2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JAM-A/F11R antigen affinity purified polyclonal antibody (Catalog # A02068-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JAM-A/F11R at approximately 41KD. The expected band size for JAM-A/F11R is at 41KD.
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Figure 2. IF analysis of JAM-A/F11R using anti-JAM-A/F11R antibody (A02068-2).
JAM-A/F11R was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-JAM-A/F11R Antibody (A02068-2) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of HEPG2 cells using anti-JAM-A/F11R antibody (A02068-2).
Overlay histogram showing HEPG2 cells stained with A02068-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-JAM-A/F11R Antibody (A02068-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For F11R (Source: Uniprot.org, NCBI)
Gene Name
F11R
Full Name
Junctional adhesion molecule A
Weight
32.424kDa
Superfamily
immunoglobulin superfamily
Alternative Names
Interleukin-17F; IL-17F; Il17f; F11r|9130004G24, AA638916, ESTM3, ESTM33, JAM, JAM-, JAM-1, JAM-A, Jcam, Jcam1, Ly10, Ly106|F11 receptor|junctional adhesion molecule A|BV11 |F11 receptor/F11R|junction cell adhesion molecule A|junction cell adhesion molecule1|junctional adhesion molecule 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on F11R, check out the F11R Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for F11R: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-JAM-A/F11R Antibody Picoband® (A02068-2)
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