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Product Info Summary
| SKU: | PA1871 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, ICC, WB |
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Product info
Product Name
Anti-ABCG5 Antibody Picoband®
SKU/Catalog Number
PA1871
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ABCG5 Antibody catalog # PA1871. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ABCG5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1871)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human ABCG5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1871 is reactive to ABCG5 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
73 kDa
Calculated molecular weight
72504 MW
Background of Abcg5
ABCG5 (Atp-binding cassette, subfamily g, member 5) also known as STEROLIN 1, is a protein that in humans is encoded by the ABCG5 gene. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. This protein is a member of the White subfamily. The protein encoded by this gene functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. The ABCG5 gene contains 13 exons and spans about 28 kb.The ABCG5 gene is mapped on 2p21. It is expressed in a tissue-specific manner in the liver, colon, and intestine. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG8. Mutations in this gene may contribute to sterol accumulation and atheroschlerosis, and have been observed in patients with sitosterolemia. Small (2003) reviewed the role of ABC transporters in secretion of cholesterol from liver into bile, particularly the role of ABCG5/ABCG8.The ABCG5 and ABCG8 genes are an example of closely neighboring genes in a head-to-head orientation that, when mutated, cause the same phenotype.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1871 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: MCF-7 Whole Cell, A549 Whole Cell, HT1080 Whole Cell, U87 Whole Cell, SKOV Whole Cell
FCM: A431 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ABCG5 using anti-ABCG5 antibody (PA1871).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: MCF-7 Whole Cell Lysate
Lane 2: A549 Whole Cell Lysate
Lane 3: HT1080 Whole Cell Lysate
Lane 4: U87 Whole Cell Lysate
Lane 5: SKOV Whole Cell Lysate
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCG5 antigen affinity purified polyclonal antibody (Catalog # PA1871) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCG5 at approximately 73KD. The expected band size for ABCG5 is at 73KD.
Click image to see more details
Figure 2. Flow Cytometry analysis of A431 cells using anti-ABCG5 antibody (PA1871).
Overlay histogram showing A431 cells stained with PA1871 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCG5 Antibody (PA1871,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For ABCG5 (Source: Uniprot.org, NCBI)
Gene Name
ABCG5
Full Name
ATP-binding cassette sub-family G member 5
Weight
72504 MW
Superfamily
ABC transporter superfamily
Alternative Names
ATP-binding cassette sub-family G member 5;Sterolin-1;ABCG5; Abcg5|Ster, cm, cmp, sterolin-1, tr, trac|ATP binding cassette subfamily G member 5|ATP-binding cassette sub-family G member 5|ATP-binding cassette, sub-family G (WHITE), member 5|sterolin 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ABCG5, check out the ABCG5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ABCG5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ABCG5 Antibody Picoband® (PA1871)
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Customer Q&As
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7 Customer Q&As for Anti-ABCG5 Antibody Picoband®
Question
Is this PA1871 anti-ABCG5 antibody reactive to the isotypes of ABCG5?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
The immunogen of PA1871 anti-ABCG5 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human ABCG5(116-131aa NGRALRREQFQDCFSY). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-16
Question
Do you have a BSA free version of anti-ABCG5 antibody PA1871 available?
Z. Mangal
Verified customer
Asked: 2019-08-08
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-ABCG5 antibody PA1871 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-08
Question
Would anti-ABCG5 antibody PA1871 work on canine Flow Cytometry with liver?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
Our lab technicians have not tested anti-ABCG5 antibody PA1871 on canine. You can run a BLAST between canine and the immunogen sequence of anti-ABCG5 antibody PA1871 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine liver in Flow Cytometry, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-04
Question
We need to test anti-ABCG5 antibody PA1871 on human liver for research purposes, then I may be interested in using anti-ABCG5 antibody PA1871 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Kulkarni
Verified customer
Asked: 2019-05-27
Answer
The products we sell, including anti-ABCG5 antibody PA1871, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-27
Question
Is a blocking peptide available for product anti-ABCG5 antibody (PA1871)?
Verified Customer
Verified customer
Asked: 2018-08-08
Answer
We do provide the blocking peptide for product anti-ABCG5 antibody (PA1871). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-08-08
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-ABCG5 antibody PA1871. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-05-17
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-17
Question
I have a question about product PA1871, anti-ABCG5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
W. Taylor
Verified customer
Asked: 2017-05-10
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1871 anti-ABCG5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-05-10
