F11r Antibodies

Junctional adhesion molecule A (F11r)

Appears to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3 (PubMed:11447115).

The institution of the PARD6- PARD3 complicated may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (PubMed:11447115). Plays a role in regulating monocyte transmigration involved with ethics of epithelial barrier (PubMed:9660867).

Gene Information

Mouse
Gene Name:	F11r
Uniprot:	O88792
Entrez:	16456

Family

Belongs to:
immunoglobulin superfamily

Alternative Names

CD321 antigen; CD321; F11 receptor; F11R; JAM-1; JAM1CD321; JAMA; JAM-A; JCAMJAM; Junctional adhesion molecule 1Platelet F11 receptor; junctional adhesion molecule A; PAM-1; PAM-1KAT; Platelet adhesion molecule 1

Molecular Weight

Mass (kDA):
32.424 kDA

Genomic Context

Mouse
Location:	1 H3|1 79.43 cM
Sequence:	1;

Diseases

• Tissue Adhesions
• Inflammation
• Tumor Angiogenesis
• Neoplasms
• Malignant Neoplasms
• Atherosclerosis
• Thrombosis
• Inflammatory Thrombosis
• Neoplasm Metastasis
• Carcinoma
• Mammary Neoplasms
• Pathologic Neovascularization
• Cell Invasion

• Malignant Neoplasm Of Breast
• Hyperlipidemia
• Cerebrovascular Accident
• Reoviridae Infections
• Cardiovascular Diseases
• Neoplasm Invasiveness
• Extravasation

Pathways

• Cell Adhesion
• Angiogenesis
• Cell Migration
• Pathogenesis
• Secretion
• Platelet Aggregation
• Localization
• Cell-cell Adhesion
• Immune Response
• Diapedesis
• Platelet Activation
• Tropism
• Cell Motility

• Endothelial Cell Migration
• Leukocyte Migration
• Tight Junction Assembly
• Chemotaxis
• Translation
• Sperm Motility
• Transport

PTMs

• Phosphorylation
• Reduction

• Cleavage
• Oxidation

• Dephosphorylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/F11r

Boster Bio Antibodies - Best Uses Of The F11R Marker

If you're looking for more information about the Boster Bio, here you'll find it. You'll find out about the history of the marker, the optimization strategies, troubleshooting guidelines, and Antibodies test on WB. This article will also give you a basic overview of the F11R marker and how to utilize it for your next antibody project. This article also provides important information on this well-known marker.

Boster Bio's history

Boster Bio was founded by Steven Xia, a renowned histologist. They specialize in WB/IHC compatible antigen kits and high-sensitivity ELISA kit kits. Boster makes use of the most advanced technology and APIs in the creation of these high-quality products. Find out more about the history of Boster Bio. Boster Bio also offers customized antibodies for specific uses. It has a history of high-quality products, superior customer services, and price points that are competitive.

The company's success can be traced to its innovative antibody development. The company came up with its first product in 1993, earning Steven Boster the nickname, "the science convert in the lavatory." The team developed a number of products, including hundreds of primary antibodies, and grew to the largest catalog of Chinese antibodies company in the late 90s. PicoKine(tm), one of the Boster Bio proprietary ELISA platform offers high-sensitivity ELISA kit that can be used in a wide variety of applications.

Optimization tips

Boster Bio guides can assist you in avoiding common pitfalls when optimizing your experiments using the F11R marker. Boster Bio's optimization guidelines can assist you in making informed choices to optimize your experiments for the best results. There are always problems with experiments. The good news is that proper control can eliminate many of these sources of errors. Troubleshooting guides can also help you figure out the root of the problem and what you can do to repair it.

Antibodies are tested on WB

There are a variety of options when it comes to selecting the flow method for your antibody. Boster provides high-affinity antibodies which are extensively referenced in scientific publications. They are also tested on ELISA, Western Blotting, and Immunohistochemistry. Boster also honors scientists who submit their research to its review process. They can be assured that the antibodies they receive will be most effective and accurate for their experiments.

Monoclonal antibody selection is a challenge without specificity. This is often proved by a proper control. Antibodies that have a single band are highly specific for the target protein. To ensure their specificity, lab technicians design multiple controls for each antibody. If the controls are positive, it means that the antibody has been designed for the protein that it targets. Once selected, antibodies undergo further application testing.

The PfRipr interacting protein is an enormous cysteine-rich protein that is located in the micronemes of Plasmodium falciparum. It is composed of 10 EGF-like domains. The protein is transformed into two polypeptides each of which is 65 kDa. These two polypeptides go into the supernatant when invaded and have been associated with protection against malaria in clinical cases.

IHC

The F11R marker has been utilized in IHC for a variety of reasons. The marker can be used to determine the adhesion of platelets towards inflamed endothelial cells (ECs). Incredibly, the de-novo expression of F11R is critical for the formation of plaques in denuded blood vessels. This evidence is a strong confirmation of the importance of F11R in the development of atherosclerosis.

The F11R protein has molecular weight of 37 kDa. It was found in the media following IFNg and TNFa treatment. F11R expression in untreated arterial and venous cells and in treated endothelial cell has also been observed. These results suggest that F11R is a useful IHC marker for endothelial cell expression in human disease.

Anti-F11R siRNA blocks F11R protein synthesis in endothelial cells that have been treated with TNFa. F11R expression was not inhibited by siRNAs that targets F11R in HUVEC. This could be the reason why F11R can be useful in a variety of ways. It isn't clear which F11R siRNA applications are most advantageous.

ICC

Knockdown of the F11R gene via transfection using lentiviral in the pancreatic cancer cell line PANC-1 can effectively silence the gene, thereby reducing metastasis and cell proliferation. Patients with high F11R expression may experience knockdown of their F11R gene a valuable therapeutic option. Further research is needed to discover the molecular process that triggers these actions. Further research is required to determine the optimal use of this marker in pancreatic carcinoma.

The TCGA database includes pancreatic cancer cell lines that have been cultured in DMEM medium containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. The cells were then placed in 6-well plates. The cells were harvested after 72 h and 120 hours. Quantitative RT-PCR was employed to determine knockdown efficacy.

There are conflicting data regarding F11R's role in pancreatic cancer. Some believe that the gene acts in pancreatic cancer cells as an oncogene. Knockdown of the F11R gene lays the groundwork for molecular study of the pancreatic cancer's pathway. More research is needed to understand the role played by the F11R gene for pancreatic cancer.

Immunofluorescence

The F11R marker has many applications in biomedical research. It is a tool to detect proteins that are expressed in human cells. It is used in immunohistochemistry, western blotting, and ELISA. Boster Bio provides high-affinity antibodies that have been tested in a wide range of applications. If you're unsure which antibody you should use then you can refer to our troubleshooting guides to determine the most appropriate solution for your research.

Glioblastomas, a rare but deadly type of brain tumor are extremely uncommon. They have extremely low survival rates. Patients suffering from this type of cancer have a distinct biomarker that predicts their survival rate The F11R protein. Researchers believe that this will allow them to find new treatments for patients suffering from this disease. Although the results of these studies are encouraging further research is required to determine which drugs are effective.