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Product Info Summary
| SKU: | A01482-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-ABCG8 Antibody Picoband®
SKU/Catalog Number
A01482-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ABCG8 Antibody Picoband® catalog # A01482-1. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ABCG8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01482-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human ABCG8, different from the related mouse and rat sequences by twelve amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01482-1 is reactive to ABCG8 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
76 kDa
Calculated molecular weight
75679 MW
Background of ABCG8
ATP-binding cassette sub-family G member 8 is a protein that in humans is encoded by the ABCG8 gene. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The protein encoded by this gene functions to exclude non-cholesterol sterol entry at the intestinal level, promote excretion of cholesterol and sterols into bile, and to facilitate transport of sterols back into the intestinal lumen. It is expressed in a tissue-specific manner in the liver, intestine, and gallbladder. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG5. Mutations in this gene may contribute to sterol accumulation and atherosclerosis, and have been observed in patients with sitosterolemia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01482-1 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Positive Control
WB: human HL-60 whole cell, human K562 whole cell, human HepG2 whole cell, rat liver tissue, rat PC-12 whole cell, mouse liver tissue, mouse HEPA1-6 whole cell
ICC/IF: HepG2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ABCG8 using anti-ABCG8 antibody (A01482-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCG8 antigen affinity purified polyclonal antibody (Catalog # A01482-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCG8 at approximately 76 kDa. The expected band size for ABCG8 is at 76 kDa.
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Figure 2. IF analysis of ABCG8 using anti-ABCG8 antibody (A01482-1).
ABCG8 was detected in an immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ABCG8 Antibody (A01482-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For ABCG8 (Source: Uniprot.org, NCBI)
Gene Name
ABCG8
Full Name
ATP-binding cassette sub-family G member 8
Weight
75679 MW
Superfamily
ABC transporter superfamily
Alternative Names
ATP-binding cassette sub-family G member 8;Sterolin-2;ABCG8; ABCG8 GBD4, STSL, STSL1 ATP binding cassette subfamily G member 8 ATP-binding cassette sub-family G member 8|ATP-binding cassette, sub-family G (WHITE), member 8|sterolin 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ABCG8, check out the ABCG8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ABCG8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ABCG8 Antibody Picoband® (A01482-1)
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Customer Q&As
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5 Customer Q&As for Anti-ABCG8 Antibody Picoband®
Question
I have attached the WB image, lot number and protocol we used for liver using anti-ABCG8 antibody A01482-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-10
Question
Our lab want to know about to test anti-ABCG8 antibody A01482-1 on human liver for research purposes, then I may be interested in using anti-ABCG8 antibody A01482-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
The products we sell, including anti-ABCG8 antibody A01482-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-14
Question
I have a question about product A01482-1, anti-ABCG8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01482-1 anti-ABCG8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-10
Question
Will anti-ABCG8 antibody A01482-1 work on pig WB with right lobe of liver?
Verified Customer
Verified customer
Asked: 2018-12-25
Answer
Our lab technicians have not validated anti-ABCG8 antibody A01482-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-ABCG8 antibody A01482-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig right lobe of liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-25
Question
Is a blocking peptide available for product anti-ABCG8 antibody (A01482-1)?
Verified Customer
Verified customer
Asked: 2018-11-26
Answer
We do provide the blocking peptide for product anti-ABCG8 antibody (A01482-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-11-26
