Anti-JAK2 (Ab-570) Antibody

Product Name

Anti-JAK2 (Ab-570) Antibody

View all JAK2 Antibodies

SKU/Catalog Number

A00027

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-JAK2 (Ab-570) Antibody (Catalog # A00027). Tested in WB, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-JAK2 (Ab-570) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00027)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized non-phosphopeptide derived from human JAK2 around the phosphorylation site of tyrosine 570 (G-D-Y(p)-G-Q).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Weakly cross-reacts with dog p53.

Reactive Species

A00027 is reactive to JAK2 in Human, Mouse

Applications

A00027 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

130674 MW

Background of JAK2

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation. Plays a role in cell cycle by phosphorylating CDKN1B. Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin.

Saltzman A., Biochem. Biophys. Res. Commun. 246:627-633(1998).
Dalal I., Blood 91:844-851(1998).
Peeters P., Blood 90:2535-2540(1997)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

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Western blot analysis of extracts from 293 cells, treated with etoposide (25uM, 24hours), using JAK2 (Ab-570) antibody A00027.

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Immunofluorescence analysis of NIH/3T3 cells, using JAK2 (Ab-570) antibody A00027.

Gene/Protein Information For JAK2 (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.7.10; EC 2.7.10.2; Jak2; JAK-2; Janus kinase 2Janus kinase 2 (a protein tyrosine kinase); JTK10; tyrosine-protein kinase JAK2 JAK2 JTK10 Janus kinase 2 tyrosine-protein kinase JAK2|JAK-2|Janus kinase 2 (a protein tyrosine kinase)

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In this infographic, you will see the following information for JAK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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