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Product Info Summary
| SKU: | M00027 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-JAK2 Monoclonal Antibody
SKU/Catalog Number
M00027
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-JAK2 Monoclonal Antibody catalog # M00027. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-JAK2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00027)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
COG-10
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human JAK2 Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00027 is reactive to JAK2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
131 kDa
Calculated molecular weight
88068 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00027 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50-100
Positive Control
WB: human HepG2 whole cell, human THP-1 whole cell, rat NRK whole cell, rat PC-12 whole cell, mouse RAW2647 whole cell
IHC: human prostate cancer tissue
ICC/IF: A549 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of JAK2 using anti-JAK2 antibody (M00027).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: rat NRK whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JAK2 antigen affinity purified monoclonal antibody (Catalog # M00027) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JAK2 at approximately 131 kDa. The expected band size for JAK2 is at 131 kDa.
Click image to see more details
Figure 2. IHC analysis of JAK2 using anti-JAK2 antibody (M00027).
JAK2 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-JAK2 Antibody (M00027) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IF analysis of JAK2 using anti-JAK2 antibody (M00027) and anti-Beta Tubulin antibody (M01857-3).
JAK2 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:50 with rabbit anti-JAK2 Antibody (M00027) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For JAK2 (Source: Uniprot.org, NCBI)
Gene Name
JAK2
Full Name
Tyrosine-protein kinase JAK2
Weight
88068 MW
Superfamily
protein kinase superfamily
Alternative Names
Signal transducer and activator of transcription 3;Acute-phase response factor;STAT3;APRF; JAK2 JTK10 Janus kinase 2 tyrosine-protein kinase JAK2|JAK-2|Janus kinase 2 (a protein tyrosine kinase)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on JAK2, check out the JAK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for JAK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-JAK2 Monoclonal Antibody (M00027)
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Customer Q&As
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17 Customer Q&As for Anti-JAK2 Monoclonal Antibody
Question
Hi, I have a customer looking for a monoclonal JAK2 antibody that does NOT cross with mouse. They are asking about the immunogen for Anti-JAK2 Monoclonal Antibody (catalog# M00027). The datasheet says it reacts with human/mouse/rat samples but only human data is shown, so maybe it doesn’t cross with mouse(?) Can you give me the amino acid sequence used as immunogen? I will BLAST it for mouse cross. Or let me know what percent homology the sequence has in mouse?
Verified customer
Asked: 2022-08-01
Answer
Thanks for your inquiry. The immunogen sequence for M00027 is RPSFRDLALRVDQIRD. Please see the updated image for mouse cell sample on the product page. https://www.bosterbio.com/anti-jak2-antibody-m00027-boster.html
Boster Scientific Support
Answered: 2022-08-01
Question
Is there a BSA free version of anti-JAK2 Monoclonal antibody M00027 available?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-JAK2 Monoclonal antibody M00027 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-05-06
Question
Will M00027 anti-JAK2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
You can see on the product datasheet, M00027 anti-JAK2 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-05-06
Question
Is a blocking peptide available for product anti-JAK2 Monoclonal antibody (M00027)?
J. Anderson
Verified customer
Asked: 2020-04-07
Answer
We do provide the blocking peptide for product anti-JAK2 Monoclonal antibody (M00027). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-07
Question
Is this M00027 anti-JAK2 Monoclonal antibody reactive to the isotypes of JAK2?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
The immunogen of M00027 anti-JAK2 Monoclonal antibody is A synthesized peptide derived from human JAK2 Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-02
Question
We are currently using anti-JAK2 Monoclonal antibody M00027 for rat tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
The anti-JAK2 Monoclonal antibody (M00027) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-07
Question
I see that the anti-JAK2 Monoclonal antibody M00027 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-25
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain using anti-JAK2 Monoclonal antibody M00027. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-03
Question
We have observed staining in mouse erythroleukemia. Any tips? Is anti-JAK2 Monoclonal antibody supposed to stain erythroleukemia positively?
G. Huang
Verified customer
Asked: 2019-11-06
Answer
According to literature erythroleukemia does express JAK2. According to Uniprot.org, JAK2 is expressed in popliteal artery, fetal brain, erythroleukemia, among other tissues. Regarding which tissues have JAK2 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 10899310
Boster Scientific Support
Answered: 2019-11-06
Question
I would like using your anti-JAK2 Monoclonal antibody for response to antibiotic studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
Thanks for your inquiry. This M00027 anti-JAK2 Monoclonal antibody is validated on jurkat cell lysate. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-07-30
Question
My question regarding product M00027, anti-JAK2 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00027 anti-JAK2 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-25
Question
Our lab used your anti-JAK2 Monoclonal antibody for WB on erythroleukemia in a previous experiment. I am using mouse, and We intend to use the antibody for IF next. We need examining erythroleukemia as well as popliteal artery in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-03-26
Answer
I looked at the website and datasheets of our anti-JAK2 Monoclonal antibody and I see that M00027 has been validated on mouse in both WB and IF. Thus M00027 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-03-26
Question
My team were content with the WB result of your anti-JAK2 Monoclonal antibody. However we have observed positive staining in popliteal artery endomembrane system using this antibody. Is that expected? Could you tell me where is JAK2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-08-31
Answer
According to literature, popliteal artery does express JAK2. Generally JAK2 expresses in endomembrane system. Regarding which tissues have JAK2 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 10899310
Boster Scientific Support
Answered: 2018-08-31
Question
I was wanting to use to test anti-JAK2 Monoclonal antibody M00027 on human fetal brain for research purposes, then I may be interested in using anti-JAK2 Monoclonal antibody M00027 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-11-16
Answer
The products we sell, including anti-JAK2 Monoclonal antibody M00027, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-16
Question
I was wanting to use your anti-JAK2 Monoclonal antibody for WB for human fetal brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human fetal brain identification?
H. Banerjee
Verified customer
Asked: 2017-10-16
Answer
You can see on the product datasheet, M00027 anti-JAK2 Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human fetal brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-16
Question
Please see the WB image, lot number and protocol we used for fetal brain using anti-JAK2 Monoclonal antibody M00027. Please let me know if you require anything else.
P. Mitchell
Verified customer
Asked: 2013-09-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-09-26
Question
Would anti-JAK2 Monoclonal antibody M00027 work for WB with fetal brain?
R. Johnson
Verified customer
Asked: 2013-09-02
Answer
According to the expression profile of fetal brain, JAK2 is highly expressed in fetal brain. So, it is likely that anti-JAK2 Monoclonal antibody M00027 will work for WB with fetal brain.
Boster Scientific Support
Answered: 2013-09-02
