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Product Info Summary
| SKU: | M00007 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-STAT3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00007
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-STAT3 Rabbit Monoclonal Antibody catalog # M00007. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-STAT3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00007)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AIH-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human STAT3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00007 is reactive to STAT3 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
88 kDa
Calculated molecular weight
88 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00007 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:100-1:500
FC 1:200-1:500
Positive Control
IHC: human thyroid carcinoma tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of STAT3 using anti-STAT3 antibody (M00007).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT3 antigen affinity purified monoclonal antibody (Catalog # M00007) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT3 at approximately 88 kDa. The expected band size for STAT3 is at 88 kDa.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma, using STAT3 Antibody.
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Immunofluorescent analysis of HeLa cells, using STAT3 Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
Protein Target Info & Infographic
Gene/Protein Information For STAT3 (Source: Uniprot.org, NCBI)
Gene Name
STAT3
Full Name
Signal transducer and activator of transcription 3
Weight
88 kDa
Superfamily
transcription factor STAT family
Alternative Names
Signal transducer and activator of transcription 3;Acute-phase response factor;STAT3;APRF; STAT3 ADMIO, ADMIO1, APRF, HIES signal transducer and activator of transcription 3 signal transducer and activator of transcription 3|DNA-binding protein APRF|acute-phase response factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STAT3, check out the STAT3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STAT3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-STAT3 Rabbit Monoclonal Antibody (M00007)
Hello CJ!
M00007 has been cited in 9 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Saturated hydrogen alleviates CCl4-induced acute kidney injury via JAK2/STAT3/p65 signaling:
He J,Zhang W,Di T,Meng J,Qi Y,Li G,Zhang Y,Su H,Yan W.Water extract of sporoderm-broken spores of Ganoderma lucidum enhanced pd-l1 antibody efficiency through downregulation and relieved complications of pd-l1 monoclonal antibody.Biomed Pharmacother.2020
Species: Human,Mouse
M00007 usage in article: APP:IHC, SAMPLE:SPLEEN,HEART,LUNG AND KIDNEY, DILUTION:NA
Zhang Q,Duan HX,Li RL,Sun JY,Liu J,Peng W,Wu CJ,Gao YX. Inducing Apoptosis and Suppressing Inflammatory Reactions in Synovial Fibroblasts are Two Important Ways for Guizhi-Shaoyao-Zhimu Decoction Against Rheumatoid Arthritis. J Inflamm Res.2021 Jan 26;14:
Species: Human
M00007 usage in article: APP:WB, SAMPLE:MH7A CELL, DILUTION:1:300
Protective effects of Tongxinluo on cerebral ischemia/reperfusion injury related to Connexin 43/Calpain II/Bax/Caspase-3 pathway in rat
IL-6 Inhibits Starvation-induced Autophagy via the STAT3/Bcl-2 Signaling Pathway
Delivery of the co-expression plasmid pEndo-Si-Stat3 by attenuatedSalmonellaserovartyphimuriumfor prostate cancer treatment
Prognostic role of STAT3 in solid tumors: a systematic review and meta-analysis
Hu T, Zhang C, Tang Q, Su Y, Li B, Chen L, Zhang Z, Cai T, Zhu Y. Bmc Cancer. 2013 May 22;13:251. Doi: 10.1186/1471-2407-13-251. Variant G6Pd Levels Promote Tumor Cell Proliferation Or Apoptosis Via The Stat3/5 Pathway In The Human Melanoma Xenogr...
Khan M, Li T, Ahmad Khan Mk, Rasul A, Nawaz F, Sun M, Zheng Y, Ma T. Biomed Res Int. 2013;2013:719858. Doi: 10.1155/2013/719858. Epub 2012 Dec 27. Alantolactone Induces Apoptosis In Hepg2 Cells Through Gsh Depletion, Inhibition Of Stat3 Activation...
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-STAT3 Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-STAT3 Rabbit Monoclonal antibody for IF for rat kidney pancreas on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat kidney pancreas identification?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
It shows on the product datasheet, M00007 anti-STAT3 Rabbit Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat kidney pancreas in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-12
Question
Is this M00007 anti-STAT3 Rabbit Monoclonal antibody reactive to the isotypes of STAT3?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
The immunogen of M00007 anti-STAT3 Rabbit Monoclonal antibody is A synthesized peptide derived from human STAT3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-20
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney pancreas using anti-STAT3 Rabbit Monoclonal antibody M00007. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-29
Question
I see that the anti-STAT3 Rabbit Monoclonal antibody M00007 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-23
Question
I have a question about product M00007, anti-STAT3 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
N. Kulkarni
Verified customer
Asked: 2019-08-28
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00007 anti-STAT3 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-28
Question
We have been able to see staining in human cervix carcinoma. What should we do? Is anti-STAT3 Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
From what I have seen in literature cervix carcinoma does express STAT3. From what I have seen in Uniprot.org, STAT3 is expressed in upper lobe of lung, placenta, kidney pancreas, liver, cervix carcinoma, erythroleukemia, among other tissues. Regarding which tissues have STAT3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Erythroleukemia, Pubmed ID: 23186163
Kidney, and Pancreas, Pubmed ID: 15489334
Liver, Pubmed ID: 7701321, 24275569
Placenta, Pubmed ID: 7512451
Boster Scientific Support
Answered: 2019-08-13
Question
Would M00007 anti-STAT3 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
It shows on the product datasheet, M00007 anti-STAT3 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-29
Question
Is there a BSA free version of anti-STAT3 Rabbit Monoclonal antibody M00007 available?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-STAT3 Rabbit Monoclonal antibody M00007 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-15
Question
Is a blocking peptide available for product anti-STAT3 Rabbit Monoclonal antibody (M00007)?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
We do provide the blocking peptide for product anti-STAT3 Rabbit Monoclonal antibody (M00007). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-11
Question
We are currently using anti-STAT3 Rabbit Monoclonal antibody M00007 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-02-26
Answer
The anti-STAT3 Rabbit Monoclonal antibody (M00007) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-26
Question
We are interested in to test anti-STAT3 Rabbit Monoclonal antibody M00007 on rat kidney pancreas for research purposes, then I may be interested in using anti-STAT3 Rabbit Monoclonal antibody M00007 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Lewis
Verified customer
Asked: 2019-02-25
Answer
The products we sell, including anti-STAT3 Rabbit Monoclonal antibody M00007, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-02-25
Question
We purchased anti-STAT3 Rabbit Monoclonal antibody for IF on kidney pancreas in a previous project. I am using mouse, and We want to use the antibody for WB next. I am interested in examining kidney pancreas as well as placenta in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2017-06-19
Answer
I looked at the website and datasheets of our anti-STAT3 Rabbit Monoclonal antibody and I see that M00007 has been tested on mouse in both IF and WB. Thus M00007 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-06-19
Question
Does anti-STAT3 Rabbit Monoclonal antibody M00007 work for IF with kidney pancreas?
K. Brown
Verified customer
Asked: 2017-04-28
Answer
According to the expression profile of kidney pancreas, STAT3 is highly expressed in kidney pancreas. So, it is likely that anti-STAT3 Rabbit Monoclonal antibody M00007 will work for IF with kidney pancreas.
Boster Scientific Support
Answered: 2017-04-28
Question
See below the WB image, lot number and protocol we used for kidney pancreas using anti-STAT3 Rabbit Monoclonal antibody M00007. Please let me know if you require anything else.
M. Parker
Verified customer
Asked: 2016-12-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-12-30
Question
My boss were happy with the WB result of your anti-STAT3 Rabbit Monoclonal antibody. However we have observed positive staining in placenta cytoplasm. nucleus using this antibody. Is that expected? Could you tell me where is STAT3 supposed to be expressed?
D. Roberts
Verified customer
Asked: 2016-11-04
Answer
From literature, placenta does express STAT3. Generally STAT3 expresses in cytoplasm. nucleus. Regarding which tissues have STAT3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Erythroleukemia, Pubmed ID: 23186163
Kidney, and Pancreas, Pubmed ID: 15489334
Liver, Pubmed ID: 7701321, 24275569
Placenta, Pubmed ID: 7512451
Boster Scientific Support
Answered: 2016-11-04
Question
We need using your anti-STAT3 Rabbit Monoclonal antibody for regulation of feeding behavior studies. Has this antibody been tested with western blotting on a431 cell lysate? We would like to see some validation images before ordering.
N. Dhar
Verified customer
Asked: 2015-09-18
Answer
We appreciate your inquiry. This M00007 anti-STAT3 Rabbit Monoclonal antibody is tested on a431 cell lysate, raji cell lysate. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-09-18
