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Product Info Summary
| SKU: | MP00027 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal Antibody
SKU/Catalog Number
MP00027
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal Antibody catalog # MP00027. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MP00027)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IFE-10
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-JAK2 (Y1007 + Y1008)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
MP00027 is reactive to JAK2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
78 kDa
Calculated molecular weight
26053 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MP00027 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:30
Positive Control
WB: Jurkat cell
IHC: human cervix cancer tissue
ICC/IF: Jurkat cell
Validation Images & Assay Conditions
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Western blot analysis of JAK2 phosphorylation expression in Jurkat cell lysates treated with Pervanadate.
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Immunohistochemical analysis of paraffin-embedded human cervix cancer, using Phospho-JAK2 (Y1007 + Y1008) Antibody.
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Immunofluorescent analysis of Jurkat cells treated with Pervanadate, using Phospho-JAK2 (Y1007 + Y1008) Antibody
Protein Target Info & Infographic
Gene/Protein Information For JAK2 (Source: Uniprot.org, NCBI)
Gene Name
JAK2
Full Name
Tyrosine-protein kinase JAK2
Weight
26053 MW
Superfamily
protein kinase superfamily
Alternative Names
Red fluorescent protein eqFP611;GFP-like chromoprotein; JAK2 JTK10 Janus kinase 2 tyrosine-protein kinase JAK2|JAK-2|Janus kinase 2 (a protein tyrosine kinase)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on JAK2, check out the JAK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for JAK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal Antibody (MP00027)
Hello CJ!
MP00027 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhu L,Liu Y,Wu X,et al.Cerebroprotein hydrolysate-I protects senescence-induced by D-galactose in PC12 cells and mice.Food Sci Nutr.2021;00:1–10.https://doi.org/10. 1002/fsn3.2333
Species: Mouse,Rat
MP00027 usage in article: APP:WB, SAMPLE:PC-12 CELL AND HIPPOCAMPUL TISSUE, DILUTION:1:1000
He J,Zhang W,Di T,Meng J,Qi Y,Li G,Zhang Y,Su H,Yan W.Water extract of sporoderm-broken spores of Ganoderma lucidum enhanced pd-l1 antibody efficiency through downregulation and relieved complications of pd-l1 monoclonal antibody.Biomed Pharmacother.2020
Species: Human,Mouse
MP00027 usage in article: APP:WB, SAMPLE:HOS CELL AND K7M2 CELL, DILUTION:1:500
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal Antibody
Question
We have tried in the past anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody for WB on erythroleukemia a few months ago. I am using mouse, and I plan to use the antibody for IP next. you antibody examining erythroleukemia as well as fetal brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
I have checked the website and datasheets of our anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody and I see that MP00027 has been validated on mouse in both WB and IP. Thus MP00027 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-03-02
Question
We are interested in to test anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody MP00027 on human fetal brain for research purposes, then I may be interested in using anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody MP00027 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
The products we sell, including anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody MP00027, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-16
Question
I have attached the WB image, lot number and protocol we used for fetal brain using anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody MP00027. Please let me know if you require anything else.
J. Krishna
Verified customer
Asked: 2019-11-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-28
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain using anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody MP00027. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-14
Question
Is there a BSA free version of anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody MP00027 available?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody MP00027 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-02
Question
Would MP00027 anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
It shows on the product datasheet, MP00027 anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-18
Question
Is a blocking peptide available for product anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody (MP00027)?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
We do provide the blocking peptide for product anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody (MP00027). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-04
Question
We have observed staining in mouse erythroleukemia. Do you have any suggestions? Is anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody supposed to stain erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2019-01-01
Answer
From literature erythroleukemia does express JAK2. From Uniprot.org, JAK2 is expressed in popliteal artery, fetal brain, erythroleukemia, among other tissues. Regarding which tissues have JAK2 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 10899310
Boster Scientific Support
Answered: 2019-01-01
Question
I see that the anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody MP00027 works with WB, what is the protocol used to produce the result images on the product page?
G. Anderson
Verified customer
Asked: 2018-04-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-04-13
Question
I have a question about product MP00027, anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-09-08
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MP00027 anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-09-08
Question
We are currently using anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody MP00027 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-18
Answer
The anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody (MP00027) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-18
Question
Is this MP00027 anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody reactive to the isotypes of JAK2?
Verified Customer
Verified customer
Asked: 2017-07-19
Answer
The immunogen of MP00027 anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody is A synthesized peptide derived from human Phospho-JAK2 (Y1007 + Y1008). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-07-19
Question
We are interested in using your anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody for positive regulation of growth factor dependent skeletal muscle satellite cell proliferation studies. Has this antibody been tested with western blotting on jurkat cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-06-19
Answer
Thanks for your inquiry. This MP00027 anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody is tested on jurkat cells. It is guaranteed to work for Flow Cytometry, IP, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-06-19
Question
Will anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody MP00027 work for WB with fetal brain?
W. Bhatt
Verified customer
Asked: 2015-04-03
Answer
According to the expression profile of fetal brain, JAK2 is highly expressed in fetal brain. So, it is likely that anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody MP00027 will work for WB with fetal brain.
Boster Scientific Support
Answered: 2015-04-03
Question
I was wanting to use your anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody for WB for human fetal brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human fetal brain identification?
K. Moore
Verified customer
Asked: 2014-06-03
Answer
It shows on the product datasheet, MP00027 anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody has been tested for Flow Cytometry, IP, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human fetal brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-06-03
Question
Our team were well pleased with the WB result of your anti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal antibody. However we have seen positive staining in erythroleukemia endomembrane system using this antibody. Is that expected? Could you tell me where is JAK2 supposed to be expressed?
P. Baker
Verified customer
Asked: 2013-10-02
Answer
From what I have seen in literature, erythroleukemia does express JAK2. Generally JAK2 expresses in endomembrane system. Regarding which tissues have JAK2 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 10899310
Boster Scientific Support
Answered: 2013-10-02
