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Product Info Summary
| SKU: | PB9086 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Integrin beta 1/ITGB1 Antibody Picoband®
SKU/Catalog Number
PB9086
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Integrin beta 1/ITGB1 Antibody Picoband® catalog # PB9086. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Integrin beta 1/ITGB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9086)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ITGB1 recombinant protein (Position: N527-D728). Human ITGB1 shares 91% and 88% amino acid (aa) sequences identity with mouse and rat ITGB1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9086 is reactive to ITGB1 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
120-130 kDa
Calculated molecular weight
88415 MW
Background of ITGB1
Integrin beta-1, also known as CD29, is a protein that in humans is encoded by the ITGB1 gene. CD29 is an integrin unit associated with very late antigen receptors. It is known to conjoin with alpha-3 subunit to create alpha3beta1 complex that reacts to such molecules as netrin-1 and reelin. This gene contains a beta subunit that appears to be analogous to band-3 of integrin. It is mapped to 10p11.22. It is found that FER mediates crosstalk between CDH2 and CD29. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. The protein encoded by this gene is a beta subunit.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9086 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Positive Control
WB: human A431 whole cell, rat C6 whole cell
IHC: human placenta tissue, human tonsil tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ITGB1 using anti-ITGB1 antibody (PB9086).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: rat C6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGB1 antigen affinity purified polyclonal antibody (Catalog # PB9086) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGB1 at approximately 120-130 kDa. The expected band size for ITGB1 is at 88 kDa.
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Figure 2. IHC analysis of ITGB1 using anti-ITGB1 antibody (PB9086).
ITGB1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ITGB1 Antibody (PB9086) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of ITGB1 using anti-ITGB1 antibody (PB9086).
ITGB1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ITGB1 Antibody (PB9086) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For ITGB1 (Source: Uniprot.org, NCBI)
Gene Name
ITGB1
Full Name
Integrin beta-1
Weight
88415 MW
Superfamily
integrin beta chain family
Alternative Names
Integrin beta-1;Fibronectin receptor subunit beta;Glycoprotein IIa;GPIIA;VLA-4 subunit beta;CD29;ITGB1;FNRB, MDF2, MSK12; ITGB1 CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB integrin subunit beta 1 integrin beta-1|glycoprotein IIa|integrin VLA-4 beta subunit|integrin beta 1|integrin, beta 1 (fibronectin receptor, beta polypeptide, CD29 includes MDF2, MSK12)|very late activation protein, beta polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ITGB1, check out the ITGB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ITGB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Integrin beta 1/ITGB1 Antibody Picoband® (PB9086)
Hello CJ!
PB9086 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Inhibition of the peritoneal metastasis of human gastric cancer cells by dextran sulphate in vivo and in vitro
Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC‑α/ERK1/2 pathway: An in vitro investigation
Abnormalities in the basement membrane structure promote basal keratinocytes in the epidermis of hypertrophic scars to adopt a proliferative phenotype
Mechanical stiffness of liver tissues in relation to integrin β1 expression may influence the development of hepatic cirrhosis and hepatocellular carcinoma
Abnormalities in the basement membrane structure promote basal keratinocytes in the epidermis of hypertrophic scars to adopt a proliferative phenotype
Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC-?/ERK1/2 pathway: An in vitro investigation
Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats.
Isolation and morphological characterization of ovine amniotic fluid mesenchymal stem cells
Yin D, Tian L, Ye Y, Li K, Wang J, Cheng P, Chen A, Guo F, Huang H. Int J Mol Med. 2012 Apr;29(4):587-92. Doi: 10.3892/Ijmm.2011.871. Epub 2011 Dec 29. Nanog And ??-Catenin: A New Convergence Point In Epsc Proliferation And Differentiation.
Lin H, Yang Y, Wang Y, Wang L, Zhou X, Liu J, Peng D. Plos One. 2014 Jan 21;9(1):E85672. Doi: 10.1371/Journal.Pone.0085672. Ecollection 2014. Effect Of Mixed Transplantation Of Autologous And Allogeneic Microskin Grafts On Wound Healing In A Rat M...
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Customer Q&As
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1 Customer Q&As for Anti-Integrin beta 1/ITGB1 Antibody Picoband®
Question
We are currently using anti-Integrin beta 1/ITGB1 antibody PB9086 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
The anti-Integrin beta 1/ITGB1 antibody (PB9086) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-16
