Integrin beta-1 Antibodies

Integrin beta-1 (ITGB1)

Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta- 1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha- 1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen.

Integrins alpha-2/beta-1, alpha- 3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha- 10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domain names within the alternatively spliced CS-1 and CS-5 regions of fibronectin.

Gene Information

Human
Gene Name:	ITGB1
Uniprot:	P05556
Entrez:	3688

Family

Belongs to:
integrin beta chain family

Alternative Names

CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; Integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA

Molecular Weight

Mass (kDA):
88.415 kDA

Genomic Context

Human
Location:	10p11.22
Sequence:	10; NC_000010.11 (32900318..32958230, complement)

Tissue Specificity

Isoform 1 is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform 2 is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform 3 and isoform 4 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform 4, rather than isoform 3, is selectively expressed in peripheral T-cells. Isoform 3 is expressed in non- proliferating and differentiated prostate gland epithelial cells and in platelets, on the surface of erythroleukemia cells and in various hematopoietic cell lines. Isoform 5 is expressed specifically in striated muscle (skeletal and cardiac muscle).

Subcellular Localizations

Cell membrane; Single-pass type I membrane protein. Cell projection, invadopodium membrane; Single-pass type I membrane protein. Cell projection, ruffle membrane; Single-pass type I membrane protein. Recycling endosome. Melanosome. Cleavage furrow. Cell projection, lamellipodium. Cell projection, ruffle. Cell junction, focal adhesion. Cell surface. Isoform 2 does not localize to focal adhesions. Highly enriched in stage I melanosomes. Located on plasma membrane of neuroblastoma NMB7 cells. In a lung cancer cell line, in prometaphase and metaphase, localizes diffusely at the membrane and in a f

Diseases

• Tissue Adhesions
• Malignant Neoplasms
• Neoplasms
• Cell Invasion
• Tumor Angiogenesis
• Neoplasm Metastasis
• Leukemia
• Carcinoma
• Acute Promyelocytic Leukemia
• Malignant Paraganglionic Neoplasm
• Neoplasm Invasiveness
• Adenocarcinoma
• Fibrosis

• Nervousness
• Cell Transformation, Neoplastic
• Malignant Squamous Cell Neoplasm
• Acquired Immunodeficiency Syndrome
• Metastatic Malignant Neoplasm To The Peritoneum
• Pathologic Neovascularization
• Neoplasm Recurrence, Local

Interacting Proteins

• ADAM17
• ANGPTL4
• CRKL
• CSF2RB
• CX3CL1

• ENG
• FABP3
• FLNA
• ITGA2
• ITGA4

• ITGA5
• LYN
• PTPN1
• PXN
• Rab21

• TLN1
• Tln1
• Tln2

Pathways

• Cell Adhesion
• Pathogenesis
• Angiogenesis
• Cell Proliferation
• Cell Migration
• Immune Response
• Cell Death
• Fertilization
• Blastocyst Formation
• Localization
• Cell Division
• Cell Differentiation
• Cell-matrix Adhesion

• Integrin Activation
• Reverse Transcription
• Cell Cycle
• Focal Adhesion Assembly
• Cell Growth
• Cell Activation
• Macromolecule Biosynthetic Process

PTMs

• Phosphorylation

• Acetylation

• Methylation

Research Areas

• Cancer
• Cell Biology
• Cellular Markers
• Cytokine Research
• Immunology

• Mesenchymal Stem Cell Markers
• Neuronal Cell Markers
• Signal Transduction
• Stem Cell Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ITGB1

Boster Bio Antibodies - Best Uses Of The ITGB1 Marker

When researching the best antibody to use for your research, you should know the benefits of using Boster Bio's Recombinant Human ITGB1/Integrin beta-1/CD29. This article will discuss the benefits of using this antibody, as well as provide validation results for WB, IHC, and immunofluorescence. Troubleshooting is also covered, as well as some important points to consider.

Boster Bio

The ITGB1 protein is a central component of the genome. It is essential for the detection and characterization of dozens of human and animal diseases. Molecular biology techniques, such as DNA manipulation, are used in a wide variety of research applications. Boster Bio provides a comprehensive technical resource, including blogs and disease information, digital tools and a selection of antibodies. These antibodies react with the protein human and contain 5 mg of BSA.

Recombinant Human ITGB1/Integrin beta-1/CD29

The ITGB1 marker is an immunoglobulin found on cells of the immune system. The ITGB1 is responsible for the cellular immune response. In addition to its immunoglobulin function, ITGB1 also plays a crucial role in the immune system. Despite this, there are many pitfalls that can lead to the development of antibodies that target the ITGB1 protein. In addition to the risks, you may also find it challenging to find a suitable antibody. In most cases, the ITGB1 is present in high concentrations and is not readily detectable in samples. In such cases, an appropriate antibody is required.

ITGB1 is a heterodimeric protein with beta and alpha subunits. They transmit signals to cells in response to their extracellular environment and sense intracellular cues to regulate their interactions with the extracellular environment. In the context of biology, ITGB1 plays a key role in cell proliferation, cytoskeletal reorganization, and gene expression. It has been linked to angiogenesis, tumor metastasis, and embryonic development.

Interestingly, ITGB1 has a role in the regulation of proinflammatory cytokine production in autoimmune diseases. Itgb1 and Rps3 are expressed on immune cells. They play an important role in regulating the production of cytokines in autoimmune diseases. These proteins may also be used in diagnostic tests for arthritis. Further research is needed to establish the role of ITGB1 in RA.

The ITGB1 marker promotes a cellular immune response that is required for macrophage proliferation. In addition, three key regulators of ITGB1 are required for macrophage function. In a recent study, the ITGB1 gene was identified as a component of a cell that promotes anti-inflammatory responses. It is thus essential for the development of therapies that target this gene.

Validation on WB, IHC, Immunofluorescence and Immunofluorescence

The validation of antibodies is critical for WB and IHC analyses. This process demonstrates that an antibody recognizes its target. Companies have the opportunity to post their data on the internet portal Antibody-pedia. These companies are required to provide original experimental data for their antibodies. This process guarantees that commercial antibodies are compatible with the most recent immunofluorescence methods and provide accurate results.

The validation of PD-L1 IHC antibodies was performed on six human lung MCs and the embryonic kidney 293 cell line. The WB analysis involved two mg of protein from each cell line and transfer to nitrocellulose membrane. The results of the analysis were compared to the results of the IHC analysis. The results obtained from these analyses are shown below.

WB data are used as the initial screen for antibodies, but the immunogen must be purified. Because antibodies work better when the protein is in its native conformation, they do not perform well on denatured proteins. WB data are not an absolute standard for antibody binding in IHC and other assays. Hence, WB data are often insufficient to validate a WB assay.

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