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Product Info Summary
| SKU: | M00165 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-IRF3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00165
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IRF3 Rabbit Monoclonal Antibody catalog # M00165. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IRF3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00165)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GDG-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IRF3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00165 is reactive to IRF3 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
57 kDa
Calculated molecular weight
47219 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00165 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:100
Positive Control
IHC: Human Hodgkin's lymphoma tissue, Human astrocytoma tissue, Human ovarian cancer tissue, human cervix carcinoma tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.
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Figure 1. Western blot analysis of IRF3 using anti-IRF3 antibody (M00165).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human Raji whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF3 antigen affinity purified monoclonal antibody (Catalog # M00165) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRF3 at approximately 57 kDa. The expected band size for IRF3 is at 47 kDa.
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Immunohistochemical analysis of paraffin-embedded Human ovarian cancer, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human astrocytoma, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded human cervix carcinoma, using IRF3 Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis of Jurkat cells, using IRF3 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For IRF3 (Source: Uniprot.org, NCBI)
Gene Name
IRF3
Full Name
Interferon regulatory factor 3
Weight
47219 MW
Superfamily
IRF family
Alternative Names
Interferon regulatory factor 3;IRF-3;IRF3; IRF3 IIAE7 interferon regulatory factor 3 interferon regulatory factor 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IRF3, check out the IRF3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IRF3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IRF3 Rabbit Monoclonal Antibody (M00165)
Hello CJ!
M00165 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Curcumin exerts anti-inflammatory and antioxidative properties in 1-methyl-4-phenylpyridinium ion (MPP(+))-stimulated mesencephalic astrocytes by interference with TLR4 and downstream signaling pathway
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Customer Q&As
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5 Customer Q&As for Anti-IRF3 Rabbit Monoclonal Antibody
Question
We purchased anti-IRF3 Rabbit Monoclonal antibody for WB on eye kidney last year. I am using rat, and I plan to use the antibody for IF next. We need examining eye kidney as well as spleen in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
I have checked the website and datasheets of our anti-IRF3 Rabbit Monoclonal antibody and it appears that M00165 has been tested on rat in both WB and IF. Thus M00165 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-23
Question
My boss were satisfied with the WB result of your anti-IRF3 Rabbit Monoclonal antibody. However we have been able to see positive staining in liver cytoplasm using this antibody. Is that expected? Could you tell me where is IRF3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
From what I have seen in literature, liver does express IRF3. Generally IRF3 expresses in cytoplasm. Regarding which tissues have IRF3 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Eye, and Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Retina, Pubmed ID: 8524823
Spleen, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-07-31
Question
We need using your anti-IRF3 Rabbit Monoclonal antibody for positive regulation of cytokine secretion studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
G. Mitchell
Verified customer
Asked: 2018-12-04
Answer
I appreciate your inquiry. This M00165 anti-IRF3 Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-12-04
Question
We have been able to see staining in human eye kidney. Do you have any suggestions? Is anti-IRF3 Rabbit Monoclonal antibody supposed to stain eye kidney positively?
Z. Lewis
Verified customer
Asked: 2018-09-28
Answer
According to literature eye kidney does express IRF3. According to Uniprot.org, IRF3 is expressed in small intestine peyer's patch, retina, spleen, eye kidney, erythroleukemia, liver, among other tissues. Regarding which tissues have IRF3 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Eye, and Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Retina, Pubmed ID: 8524823
Spleen, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-09-28
Question
We are currently using anti-IRF3 Rabbit Monoclonal antibody M00165 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
D. Krishna
Verified customer
Asked: 2014-09-08
Answer
The anti-IRF3 Rabbit Monoclonal antibody (M00165) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-09-08
