Anti-IRF3 Rabbit Monoclonal Antibody

IRF3 antibody

Boster Bio Anti-IRF3 Rabbit Monoclonal Antibody catalog # M00165. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: M00165
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-IRF3 Rabbit Monoclonal Antibody

View all IRF3 Antibodies

SKU/Catalog Number

M00165

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-IRF3 Rabbit Monoclonal Antibody catalog # M00165. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IRF3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00165)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

GDG-9

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human IRF3

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00165 is reactive to IRF3 in Human, Mouse, Rat

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

57 kDa

Calculated molecular weight

47219 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M00165 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:1000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:100

Positive Control

IHC: Human Hodgkin's lymphoma tissue, Human astrocytoma tissue, Human ovarian cancer tissue, human cervix carcinoma tissue

Validation Images & Assay Conditions

Gene/Protein Information For IRF3 (Source: Uniprot.org, NCBI)

Gene Name

IRF3

Full Name

Interferon regulatory factor 3

Weight

47219 MW

Superfamily

IRF family

Alternative Names

Interferon regulatory factor 3;IRF-3;IRF3; IRF3 IIAE7 interferon regulatory factor 3 interferon regulatory factor 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on IRF3, check out the IRF3 Infographic

IRF3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for IRF3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00165 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Curcumin exerts anti-inflammatory and antioxidative properties in 1-methyl-4-phenylpyridinium ion (MPP(+))-stimulated mesencephalic astrocytes by interference with TLR4 and downstream signaling pathway

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5 Customer Q&As for Anti-IRF3 Rabbit Monoclonal Antibody

Question

We purchased anti-IRF3 Rabbit Monoclonal antibody for WB on eye kidney last year. I am using rat, and I plan to use the antibody for IF next. We need examining eye kidney as well as spleen in our next experiment. Do you have any suggestion on which antibody would work the best for IF?

Verified Customer

Verified customer

Asked: 2020-04-23

Answer

I have checked the website and datasheets of our anti-IRF3 Rabbit Monoclonal antibody and it appears that M00165 has been tested on rat in both WB and IF. Thus M00165 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-04-23

Question

My boss were satisfied with the WB result of your anti-IRF3 Rabbit Monoclonal antibody. However we have been able to see positive staining in liver cytoplasm using this antibody. Is that expected? Could you tell me where is IRF3 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-07-31

Answer

From what I have seen in literature, liver does express IRF3. Generally IRF3 expresses in cytoplasm. Regarding which tissues have IRF3 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Eye, and Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Retina, Pubmed ID: 8524823
Spleen, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-07-31

Question

We need using your anti-IRF3 Rabbit Monoclonal antibody for positive regulation of cytokine secretion studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.

G. Mitchell

Verified customer

Asked: 2018-12-04

Answer

I appreciate your inquiry. This M00165 anti-IRF3 Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-12-04

Question

We have been able to see staining in human eye kidney. Do you have any suggestions? Is anti-IRF3 Rabbit Monoclonal antibody supposed to stain eye kidney positively?

Z. Lewis

Verified customer

Asked: 2018-09-28

Answer

According to literature eye kidney does express IRF3. According to Uniprot.org, IRF3 is expressed in small intestine peyer's patch, retina, spleen, eye kidney, erythroleukemia, liver, among other tissues. Regarding which tissues have IRF3 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Eye, and Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Retina, Pubmed ID: 8524823
Spleen, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2018-09-28

Question

We are currently using anti-IRF3 Rabbit Monoclonal antibody M00165 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?

D. Krishna

Verified customer

Asked: 2014-09-08

Answer

The anti-IRF3 Rabbit Monoclonal antibody (M00165) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-09-08

Order DetailsPrice
M00165-M00165

100μL

$370
M00165-30ul

30μL sample

$99
M00165-carrier-free-1ml

Carrier Free

$2590

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