Click image to see more details
Product Info Summary
| SKU: | PA2001 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TRIF/TICAM1 Antibody Picoband®
View all TRIF/TICAM1 Antibodies
SKU/Catalog Number
PA2001
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRIF/TICAM1 Antibody catalog # PA2001. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRIF/TICAM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2001)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse TRIF.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2001 is reactive to Ticam1 in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
58-60 kDa
Calculated molecular weight
79230 MW
Background of TRIF/TICAM1
TICAM1 (TIR DOMAIN-CONTAINING ADAPTOR MOLECULE 1), also known as TRIF, is an adapter in responding to activation of toll-like receptors (TLRs). It mediates the rather delayed cascade of two TLR-associated signaling cascades, where the other one is dependent upon a MyD88 adapter. By genomic sequence analysis, Oshiumi et al. (2003) mapped the TICAM1 gene to chromosome 19p13.3. By coimmunoprecipitation analysis, Oshiumi et al. (2003) showed that TICAM1 interacts specifically with TLR3, but not with other TLRs. Functional analysis showed that the association of TLR3 and TICAM1 mediates dsRNA activation of IFNB, through either NFKB, AP1, or IRF3. TICAM1 activation of NFKB was found to occur predominantly through IRAK1 rather than IRAK2 . Small interfering (si)RNA blockage of TICAM1, just upstream of the TIR domain, reduced IFNB production in response to dsRNA.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2001 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
Click image to see more details
Anti-TRIF antibody, PA2001, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Mouse TICAM1,37.5KD
(162aa tag+ L551-E732)
Lane 1: Recombinant Mouse TICAM1 Protein 10ng
Lane 2: Recombinant Mouse TICAM1 Protein 5ng
Lane 3: Recombinant Mouse TICAM1 Protein 2.5ng
Lane 4: Recombinant Mouse TICAM1 Protein 1.25ng
Protein Target Info & Infographic
Gene/Protein Information For Ticam1 (Source: Uniprot.org, NCBI)
Gene Name
Ticam1
Full Name
TIR domain-containing adapter molecule 1
Weight
79230 MW
Alternative Names
TIR domain-containing adapter molecule 1;TICAM-1;Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta;TIR domain-containing adapter protein inducing IFN-beta;Ticam1;Trif; Ticam1|AW046014, AW547018, TICAM, TICAM-1, TRIF, Tr|toll-like receptor adaptor molecule 1|TIR domain-containing adapter molecule 1|TIR domain-containing adapter protein inducing IFN-beta|TIR domain-containing adaptor inducing interferon-beta|toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ticam1, check out the Ticam1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ticam1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRIF/TICAM1 Antibody Picoband® (PA2001)
Hello CJ!
PA2001 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Curcumin exerts anti-inflammatory and antioxidative properties in 1-methyl-4-phenylpyridinium ion (MPP(+))-stimulated mesencephalic astrocytes by interference with TLR4 and downstream signaling pathway
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-TRIF/TICAM1 Antibody Picoband®
Question
Is there a BSA free version of anti-TRIF/TICAM1 antibody PA2001 available?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-TRIF/TICAM1 antibody PA2001 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-02
Question
I see that the anti-TRIF/TICAM1 antibody PA2001 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-04
Question
Is this PA2001 anti-TRIF/TICAM1 antibody reactive to the isotypes of TICAM1?
J. Mitchell
Verified customer
Asked: 2019-12-20
Answer
The immunogen of PA2001 anti-TRIF/TICAM1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of mouse TRIF(693-708aa NNHMWGHTGAQSSDDK). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-20
Question
I was wanting to use your anti-TRIF/TICAM1 antibody for WB for mouse ovary testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse ovary testis identification?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
As indicated on the product datasheet, PA2001 anti-TRIF/TICAM1 antibody has been validated for WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse ovary testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-21
Question
Would PA2001 anti-TRIF/TICAM1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-12-04
Answer
It shows on the product datasheet, PA2001 anti-TRIF/TICAM1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-12-04
Question
I would like to test anti-TRIF/TICAM1 antibody PA2001 on mouse ovary testis for research purposes, then I may be interested in using anti-TRIF/TICAM1 antibody PA2001 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
V. Patel
Verified customer
Asked: 2018-11-12
Answer
The products we sell, including anti-TRIF/TICAM1 antibody PA2001, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-11-12
Question
Does anti-TRIF/TICAM1 antibody PA2001 work on zebrafish WB with ovary testis?
Verified Customer
Verified customer
Asked: 2018-05-03
Answer
Our lab technicians have not validated anti-TRIF/TICAM1 antibody PA2001 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-TRIF/TICAM1 antibody PA2001 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish ovary testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-03
