Anti-IRF3 Antibody Picoband® (monoclonal, 7B10)

IRF3 antibody

Boster Bio Anti-IRF3 Antibody Picoband® (monoclonal, 7B10) catalog # M00165-4. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: M00165-4
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IF, ICC, WB

Product Name

Anti-IRF3 Antibody Picoband® (monoclonal, 7B10)

View all IRF3 Antibodies

SKU/Catalog Number

M00165-4

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-IRF3 Antibody Picoband® (monoclonal, 7B10) catalog # M00165-4. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IRF3 Antibody Picoband® (monoclonal, 7B10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00165-4)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Monoclonal

Clone Number

7B10

Isotype

Mouse IgG2b

Immunogen

E.coli-derived mouse IRF3 recombinant protein (Position: M1-I419).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M00165-4 is reactive to Irf3 in Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

50-55 kDa

Calculated molecular weight

46659 MW

Background of IRF3

IRF3 (interferon regulatory factor 3) is a member of the interferon regulatory transcription factor (IRF) family. The IRF3 gene is mapped on 19q13.33. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. IRF3 plays an important role in the innate immune system's response to viral infection. Aggregated MAVS have been found to activate IRF3 dimerization. Although IRF3 increased transcriptional activity from an ISRE-containing promoter, expression of IRF3 as a Gal4 fusion protein did not activate expression of a chloramphenicol acetyltransferase (CAT) reporter gene containing repeats of the Gal4-binding sites. Translocation of IRF3 was accompanied by an increase in serine and threonine phosphorylation. The transcriptional activators CREBBP and EP300 coimmunoprecipitated with IRF3 only subsequent to viral infection, and the authors stated that these are also subunits of DRAF1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00165-4 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Mouse
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Mouse

Positive Control

WB: rat brain tissue, mouse lung tissue, mouse brain tissue, mouse NIH/3T3 whole cell
ICC/IF: RM-1 cell
FCM: HEPA1-6 cell

Validation Images & Assay Conditions

Gene/Protein Information For Irf3 (Source: Uniprot.org, NCBI)

Gene Name

Irf3

Full Name

Interferon regulatory factor 3

Weight

46659 MW

Superfamily

IRF family

Alternative Names

Solute carrier family 2, facilitated glucose transporter member 1; Glucose transporter type 1, erythrocyte/brain; GLUT-1; HepG2 glucose transporter; SLC2A1; GLUT1 IRF3 IIAE7 interferon regulatory factor 3 interferon regulatory factor 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Irf3, check out the Irf3 Infographic

Irf3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Irf3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
M00165-4

100μg

$370
M00165-4-10ug

10μg sample (liquid)

$99
M00165-4-Biotin

100 μg Biotin conjugated

$570
M00165-4-Cy3

100 μg Cy3 conjugated

$570
M00165-4-Dylight488

100 μg Dylight488 conjugated

$570
M00165-4-Dylight550

100 μg Dylight550 conjugated

$570
M00165-4-Dylight594

100 μg Dylight594 conjugated

$570
M00165-4-FITC

100 μg FITC conjugated

$570
M00165-4-HRP

100 μg HRP conjugated

$570
M00165-4-APC

100 μg APC conjugated

$670
M00165-4-PE

100 μg PE conjugated

$670
M00165-4-iFluor647

100 μg iFluor647 conjugated

$670
M00165-4-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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M00165-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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