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Product Info Summary
| SKU: | PA2008 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TRAF3 Antibody Picoband®
SKU/Catalog Number
PA2008
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRAF3 Antibody catalog # PA2008. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRAF3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2008)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human TRAF3, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2008 is reactive to TRAF3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
56 kDa
Calculated molecular weight
64490 MW
Background of TRAF-3
TRAF3 (TNF Receptor-Associated Factor 3), also called LAP1, CAP1, CRAF1 or CD40BP, is a protein that in humans is encoded by the TRAF3 gene. The protein encoded by this gene is a member of the TNF receptor (TNFR) associated factor (TRAF) protein family. Gross (2012) mapped the TRAF3 gene to chromosome 14q32.32 based on an alignment of the TRAF3 sequence with the genomic sequence (GRCh37). Dadgostar et al. (2003) determined that the coiled-coil domain of mouse T3jam interacted with the isoleucine zipper domain of Traf3. Oganesyan et al. (2006) demonstrated that cells lacking TRAF3 are defective in type I interferon responses activated by several different Toll-like receptors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2008 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
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Anti-TRAF3 antibody, PA2008, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human TRAF3, 30.6KD
(162aa tag+ R341-Y449)
Lane 1: Recombinant Human TRAF3 Protein 5ng
Lane 2: Recombinant Human TRAF3 Protein 2.5ng
Lane 3: Recombinant Human TRAF3 Protein 1.25ng
Protein Target Info & Infographic
Gene/Protein Information For TRAF3 (Source: Uniprot.org, NCBI)
Gene Name
TRAF3
Full Name
TNF receptor-associated factor 3
Weight
64490 MW
Superfamily
TNF receptor-associated factor family
Alternative Names
TNF receptor-associated factor 3;6.3.2.-;CAP-1;CD40 receptor-associated factor 1;CRAF1;CD40-binding protein;CD40BP;LMP1-associated protein 1;LAP1;TRAF3;CAP1, CRAF1; TRAF3 CAP-1, CAP1, CD40bp, CRAF1, IIAE5, LAP1, RNF118 TNF receptor associated factor 3 TNF receptor-associated factor 3|CD40 associated protein 1|CD40 binding protein|CD40 receptor associated factor 1|LMP1-associated protein 1|RING-type E3 ubiquitin transferase TRAF3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRAF3, check out the TRAF3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRAF3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRAF3 Antibody Picoband® (PA2008)
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No publications found for PA2008
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-TRAF3 Antibody Picoband®
Question
We are currently using anti-TRAF3 antibody PA2008 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
The anti-TRAF3 antibody (PA2008) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-21
Question
I have attached the WB image, lot number and protocol we used for frontal cortex using anti-TRAF3 antibody PA2008. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-09
Question
Is this PA2008 anti-TRAF3 antibody reactive to the isotypes of TRAF3?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
The immunogen of PA2008 anti-TRAF3 antibody is A synthetic peptide corresponding to a sequence in the middle region of human TRAF3(387-403aa RHDQMLSVHDIRLADMD), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-05
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for frontal cortex using anti-TRAF3 antibody PA2008. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-12-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-22
Question
I was wanting to use your anti-TRAF3 antibody for WB for rat frontal cortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat frontal cortex identification?
Verified Customer
Verified customer
Asked: 2017-10-09
Answer
It shows on the product datasheet, PA2008 anti-TRAF3 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat frontal cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-09
Question
Can you help my question with product PA2008, anti-TRAF3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Krishna
Verified customer
Asked: 2017-07-06
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2008 anti-TRAF3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-07-06
Question
My question regards to test anti-TRAF3 antibody PA2008 on rat frontal cortex for research purposes, then I may be interested in using anti-TRAF3 antibody PA2008 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
E. Zhao
Verified customer
Asked: 2016-02-23
Answer
The products we sell, including anti-TRAF3 antibody PA2008, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-02-23
