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Product Info Summary
| SKU: | M00165-6 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-IRF3 Mouse Monoclonal Antibody [Clone ID: OTI4D4]
SKU/Catalog Number
M00165-6
Size
100 µl
Description
Boster Bio IRF3 mouse monoclonal antibody, clone OTI4D4 (formerly 4D4). Catalog# M00165-6. Tested in FC, IF, IHC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-IRF3 Mouse Monoclonal Antibody [Clone ID: OTI4D4] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00165-6)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI4D4
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human IRF3 (NP_001562) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00165-6 is reactive to IRF3 in Human
Reconstitution
Calculated molecular weight
47.219kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00165-6 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF3 (1:500).
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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-IRF3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-IRF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either IRF3 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IRF3 antibody (M00165-6)
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Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY IRF3 using anti-IRF3 antibody (M00165-6/green
Protein Target Info & Infographic
Gene/Protein Information For IRF3 (Source: Uniprot.org, NCBI)
Gene Name
IRF3
Full Name
Interferon regulatory factor 3
Weight
47.219kDa
Superfamily
IRF family
Alternative Names
interferon regulatory factor 3; IRF3; IRF-3 IRF3 IIAE7 interferon regulatory factor 3 interferon regulatory factor 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IRF3, check out the IRF3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IRF3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IRF3 Mouse Monoclonal Antibody [Clone ID: OTI4D4] (M00165-6)
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