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Product Info Summary
| SKU: | PA1819 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Interferon regulatory factor 3 IRF3 Antibody Picoband®
SKU/Catalog Number
PA1819
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Interferon regulatory factor 3 IRF3 Antibody catalog # PA1819. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interferon regulatory factor 3 IRF3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1819)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human IRF3, different from the related mouse and rat sequences by five amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1819 is reactive to IRF3 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
47219 MW
Background of IRF3
IRF3 (interferon regulatory factor 3) is a member of the interferon regulatory transcription factor (IRF) family. The IRF3 gene is mapped on 19q13.33. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. IRF3 plays an important role in the innate immune system's response to viral infection. Aggregated MAVS have been found to activate IRF3 dimerization. Although IRF3 increased transcriptional activity from an ISRE-containing promoter, expression of IRF3 as a Gal4 fusion protein did not activate expression of a chloramphenicol acetyltransferase (CAT) reporter gene containing repeats of the Gal4-binding sites. Translocation of IRF3 was accompanied by an increase in serine and threonine phosphorylation. The transcriptional activators CREBBP and EP300 coimmunoprecipitated with IRF3 only subsequent to viral infection, and the authors stated that these are also subunits of DRAF1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1819 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Hela whole cell, human Jurkat whole cell, human Raji whole cell, human HepG2 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of IRF3 using anti-IRF3 antibody (PA1819).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human Raji whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF3 antigen affinity purified polyclonal antibody (Catalog # PA1819) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRF3 at approximately 55 kDa. The expected band size for IRF3 is at 47 kDa.
Protein Target Info & Infographic
Gene/Protein Information For IRF3 (Source: Uniprot.org, NCBI)
Gene Name
IRF3
Full Name
Interferon regulatory factor 3
Weight
47219 MW
Superfamily
IRF family
Alternative Names
Interferon regulatory factor 3;IRF-3;IRF3; IRF3 IIAE7 interferon regulatory factor 3 interferon regulatory factor 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IRF3, check out the IRF3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IRF3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Interferon regulatory factor 3 IRF3 Antibody Picoband® (PA1819)
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Customer Q&As
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16 Customer Q&As for Anti-Interferon regulatory factor 3 IRF3 Antibody Picoband®
Question
See below the WB image, lot number and protocol we used for small intestine peyer's patch using anti-IRF3 antibody PA1819. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-27
Question
I was wanting to use your anti-IRF3 antibody for WB for human small intestine peyer's patch on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human small intestine peyer's patch identification?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
It shows on the product datasheet, PA1819 anti-IRF3 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human small intestine peyer's patch in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-02
Question
Does anti-IRF3 antibody PA1819 work for WB with small intestine peyer's patch?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
According to the expression profile of small intestine peyer's patch, IRF3 is highly expressed in small intestine peyer's patch. So, it is likely that anti-IRF3 antibody PA1819 will work for WB with small intestine peyer's patch.
Boster Scientific Support
Answered: 2020-01-15
Question
Do you have a BSA free version of anti-IRF3 antibody PA1819 available?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IRF3 antibody PA1819 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-20
Question
Is this PA1819 anti-IRF3 antibody reactive to the isotypes of IRF3?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
The immunogen of PA1819 anti-IRF3 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human IRF3(409-427aa KAYLQDLVEGMDFQGPGES), different from the related mouse and rat sequences by five amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-24
Question
I have a question about product PA1819, anti-IRF3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1819 anti-IRF3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-04
Question
We were content with the WB result of your anti-IRF3 antibody. However we have observed positive staining in eye kidney cytoplasm using this antibody. Is that expected? Could you tell me where is IRF3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
According to literature, eye kidney does express IRF3. Generally IRF3 expresses in cytoplasm. Regarding which tissues have IRF3 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Eye, and Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Retina, Pubmed ID: 8524823
Spleen, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-06-11
Question
Would anti-IRF3 antibody PA1819 work on canine WB with liver?
Verified Customer
Verified customer
Asked: 2019-04-18
Answer
Our lab technicians have not validated anti-IRF3 antibody PA1819 on canine. You can run a BLAST between canine and the immunogen sequence of anti-IRF3 antibody PA1819 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-18
Question
I am interested in to test anti-IRF3 antibody PA1819 on human small intestine peyer's patch for research purposes, then I may be interested in using anti-IRF3 antibody PA1819 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-08-15
Answer
The products we sell, including anti-IRF3 antibody PA1819, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-15
Question
My question regards using your anti-IRF3 antibody for traf6 mediated irf7 activation studies. Has this antibody been tested with western blotting on jurkat whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-06-02
Answer
We appreciate your inquiry. This PA1819 anti-IRF3 antibody is tested on a549 whole cell lysate, hela whole cell lysate, jurkat whole cell lysate, 293t whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-06-02
Question
We have observed staining in human small intestine peyer's patch. Any tips? Is anti-IRF3 antibody supposed to stain small intestine peyer's patch positively?
C. Anderson
Verified customer
Asked: 2016-12-28
Answer
From literature small intestine peyer's patch does express IRF3. From Uniprot.org, IRF3 is expressed in small intestine peyer's patch, retina, spleen, eye kidney, erythroleukemia, liver, among other tissues. Regarding which tissues have IRF3 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Eye, and Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Retina, Pubmed ID: 8524823
Spleen, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2016-12-28
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for small intestine peyer's patch using anti-IRF3 antibody PA1819. Let me know if you need anything else.
G. Mangal
Verified customer
Asked: 2015-07-30
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-07-30
Question
I see that the anti-IRF3 antibody PA1819 works with WB, what is the protocol used to produce the result images on the product page?
R. Jha
Verified customer
Asked: 2015-03-30
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-03-30
Question
Is a blocking peptide available for product anti-IRF3 antibody (PA1819)?
F. Rodriguez
Verified customer
Asked: 2013-10-22
Answer
We do provide the blocking peptide for product anti-IRF3 antibody (PA1819). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-10-22
Question
Would PA1819 anti-IRF3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Singh
Verified customer
Asked: 2013-10-01
Answer
You can see on the product datasheet, PA1819 anti-IRF3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-10-01
Question
We are currently using anti-IRF3 antibody PA1819 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
J. Carter
Verified customer
Asked: 2013-09-20
Answer
The anti-IRF3 antibody (PA1819) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-09-20
