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Product Info Summary
| SKU: | M00263-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-MDA5 Monoclonal Antibody
SKU/Catalog Number
M00263-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MDA5 Monoclonal Antibody catalog # M00263-1. Tested in WB, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MDA5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00263-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADDD-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MDA5
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00263-1 is reactive to IFIH1 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
75 kDa
Calculated molecular weight
83642 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00263-1 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
Positive Control
WB: THP1 cell
Validation Images & Assay Conditions
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Western blot analysis of MDA5 expression in THP1 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For IFIH1 (Source: Uniprot.org, NCBI)
Gene Name
IFIH1
Full Name
Interferon-induced helicase C domain-containing protein 1
Weight
83642 MW
Superfamily
helicase family
Alternative Names
Serine/threonine-protein kinase TBK1;2.7.11.1;NF-kappa-B-activating kinase;T2K;TANK-binding kinase 1;TBK1;NAK; IFIH1 AGS7, Hlcd, IDDM19, MDA-5, MDA5, RLR-2, SGMRT1 interferon induced with helicase C domain 1 interferon-induced helicase C domain-containing protein 1|CADM-140 auto|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide|RIG-I-like receptor 2|RNA helicase-DEAD box protein 116|clinically amyopathic dermatomyositis auto 140 kDa|helicard|helicase with 2 CARD domains|melanoma differentiation-associated gene 5|melanoma differentiation-associated protein 5|murabutide down-regulated protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IFIH1, check out the IFIH1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IFIH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MDA5 Monoclonal Antibody (M00263-1)
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No publications found for M00263-1
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Customer Q&As
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4 Customer Q&As for Anti-MDA5 Monoclonal Antibody
Question
We are currently using anti-MDA5 Monoclonal antibody M00263-1 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
The anti-MDA5 Monoclonal antibody (M00263-1) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-10
Question
Is this M00263-1 anti-MDA5 Monoclonal antibody reactive to the isotypes of IFIH1?
Verified Customer
Verified customer
Asked: 2018-09-07
Answer
The immunogen of M00263-1 anti-MDA5 Monoclonal antibody is A synthesized peptide derived from human MDA5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-09-07
Question
Will anti-MDA5 Monoclonal antibody M00263-1 work on zebrafish WB with spleen?
K. Parker
Verified customer
Asked: 2017-02-08
Answer
Our lab technicians have not validated anti-MDA5 Monoclonal antibody M00263-1 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-MDA5 Monoclonal antibody M00263-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish spleen in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-02-08
Question
I was wanting to use your anti-MDA5 Monoclonal antibody for WB for human parotid gland on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human parotid gland identification?
T. Banerjee
Verified customer
Asked: 2013-07-01
Answer
You can see on the product datasheet, M00263-1 anti-MDA5 Monoclonal antibody has been validated for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human parotid gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-07-01
