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Product Info Summary
| SKU: | PB9696 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-IP10/CXCL10 Antibody Picoband®
View all CXCL10/IP-10/CRG-2 Antibodies
SKU/Catalog Number
PB9696
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IP10/CXCL10 Antibody Picoband® catalog # PB9696. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IP10/CXCL10 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9696)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse IP10 recombinant protein (Position: I22-P98). Mouse IP10 shares 70.1% and 77.9% amino acid (aa) sequence identity with human and rat IP10, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9696 is reactive to Cxcl10 in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
11 kDa
Calculated molecular weight
10789 MW
Background of CXCL10/IP-10/CRG-2
C-X-C motif chemokine 10 (CXCL10), also known as Interferon gamma-induced protein 10 kDa (IP-10) or small-inducible cytokine B10, is a protein that in humans is encoded by the CXCL10 gene. It is a small cytokine belonging to the CXC chemokine family. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. Luster et al. (1987) reported the isolation of an interferon-inducible gene that encodes a 98-amino acid protein called IP10. The chemokine IFN-gamma-inducible protein 10 (IP-10; CXCL10), a CXC chemokine, and its receptor, CXCR3, were found to be overexpressed in lymph node cells of EAMG rats. IP10 inhibits bone marrow colony formation, has antitumor activity in vivo, is a chemoattractant for human monocytes and T cells, and promotes T cell adhesion to endothelial cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9696 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Immunohistochemistry, 2-5 μg/ml, Mouse
ELISA, 0.1-0.5μg/ml, -
Positive Control
IHC: mouse spleen tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CXCL10 using anti-CXCL10 antibody (PB9696).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant mouse CXCL10 protein 10 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL10 antigen affinity purified polyclonal antibody (Catalog # PB9696) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL10 at approximately 11 kDa.
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Figure 2. IHC analysis of CXCL10 using anti-CXCL10 antibody (PB9696).
CXCL10 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CXCL10 Antibody (PB9696) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Cxcl10 (Source: Uniprot.org, NCBI)
Gene Name
Cxcl10
Full Name
C-X-C motif chemokine 10
Weight
10789 MW
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
C-X-C motif chemokine 10;10 kDa interferon gamma-induced protein;Gamma-IP10;IP-10;C7;Interferon-gamma induced protein CRG-2;Small-inducible cytokine B10;Cxcl10;Crg2, Ifi10, Inp10, Scyb10; CXCL10 C7, IFI10, INP10, IP-10, SCYB10, crg-2, gIP-10, mob-1 C-X-C motif chemokine ligand 10 C-X-C motif chemokine 10|10 kDa interferon gamma-induced protein|gamma IP10|interferon-inducible cytokine IP-10|protein 10 from interferon (gamma)-induced cell line|small inducible cytokine subfamily B (Cys-X-Cys), member 10|small-inducible cytokine B10
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cxcl10, check out the Cxcl10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cxcl10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IP10/CXCL10 Antibody Picoband® (PB9696)
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Customer Q&As
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5 Customer Q&As for Anti-IP10/CXCL10 Antibody Picoband®
Question
We purchased anti-IP10/CXCL10 antibody for ELISA on keratinocyte in a previous project. I am using mouse, and I plan to use the antibody for WB next. I was wanting to use examining keratinocyte as well as ectocervix in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
I looked at the website and datasheets of our anti-IP10/CXCL10 antibody and it appears that PB9696 has been validated on mouse in both ELISA and WB. Thus PB9696 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-02-12
Question
We have observed staining in mouse prostate. Do you have any suggestions? Is anti-IP10/CXCL10 antibody supposed to stain prostate positively?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
According to literature prostate does express CXCL10. According to Uniprot.org, CXCL10 is expressed in ectocervix, prostate, keratinocyte, foreskin keratinocyte, among other tissues. Regarding which tissues have CXCL10 expression, here are a few articles citing expression in various tissues:
Foreskin keratinocyte, Pubmed ID: 11559369
Keratinocyte, Pubmed ID: 10233762
Prostate, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-10-28
Question
We are currently using anti-IP10/CXCL10 antibody PB9696 for mouse tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says mouse. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
The anti-IP10/CXCL10 antibody (PB9696) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-19
Question
I am interested in using your anti-IP10/CXCL10 antibody for positive regulation of leukocyte chemotaxis studies. Has this antibody been tested with western blotting on hepa whole cell lysate? We would like to see some validation images before ordering.
R. Anderson
Verified customer
Asked: 2019-06-19
Answer
Thank you for your inquiry. This PB9696 anti-IP10/CXCL10 antibody is tested on mouse ovary tissue, tissue lysate, hepa whole cell lysate. It is guaranteed to work for ELISA, WB in mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-06-19
Question
Our lab were happy with the WB result of your anti-IP10/CXCL10 antibody. However we have seen positive staining in ectocervix secreted. using this antibody. Is that expected? Could you tell me where is CXCL10 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-09-04
Answer
Based on literature, ectocervix does express CXCL10. Generally CXCL10 expresses in secreted. Regarding which tissues have CXCL10 expression, here are a few articles citing expression in various tissues:
Foreskin keratinocyte, Pubmed ID: 11559369
Keratinocyte, Pubmed ID: 10233762
Prostate, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-09-04
