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Product Info Summary
| SKU: | PB9695 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-IP10/CXCL10 Antibody Picoband®
View all CXCL10/IP-10/CRG-2 Antibodies
SKU/Catalog Number
PB9695
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IP10/CXCL10 Antibody Picoband® catalog # PB9695. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IP10/CXCL10 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9695)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human IP10 recombinant protein (Position: V22-P98). Human IP10 shares 70.1% and 72.7% amino acid (aa) sequence identity with mouse and rat IP10, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9695 is reactive to CXCL10 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
34 kDa
Calculated molecular weight
10881 MW
Background of CXCL10/IP-10/CRG-2
C-X-C motif chemokine 10 (CXCL10), also known as Interferon gamma-induced protein 10 kDa (IP-10) or small-inducible cytokine B10, is a protein that in humans is encoded by the CXCL10 gene. It is a small cytokine belonging to the CXC chemokine family. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. Luster et al. (1987) reported the isolation of an interferon-inducible gene that encodes a 98-amino acid protein called IP10. The chemokine IFN-gamma-inducible protein 10 (IP-10; CXCL10), a CXC chemokine, and its receptor, CXCR3, were found to be overexpressed in lymph node cells of EAMG rats. IP10 inhibits bone marrow colony formation, has antitumor activity in vivo, is a chemoattractant for human monocytes and T cells, and promotes T cell adhesion to endothelial cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9695 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of IP10 using anti-IP10 antibody (PB9695).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: HELA Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IP10 antigen affinity purified polyclonal antibody (Catalog # PB9695) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IP10 at approximately 34 kDa. The expected band size for IP10 is at 11 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CXCL10 (Source: Uniprot.org, NCBI)
Gene Name
CXCL10
Full Name
C-X-C motif chemokine 10
Weight
10881 MW
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
C-X-C motif chemokine 10;10 kDa interferon gamma-induced protein;Gamma-IP10;IP-10;Small-inducible cytokine B10;CXCL10 (1-73);CXCL10;INP10, SCYB10; CXCL10 C7, IFI10, INP10, IP-10, SCYB10, crg-2, gIP-10, mob-1 C-X-C motif chemokine ligand 10 C-X-C motif chemokine 10|10 kDa interferon gamma-induced protein|gamma IP10|interferon-inducible cytokine IP-10|protein 10 from interferon (gamma)-induced cell line|small inducible cytokine subfamily B (Cys-X-Cys), member 10|small-inducible cytokine B10
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CXCL10, check out the CXCL10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CXCL10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IP10/CXCL10 Antibody Picoband® (PB9695)
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Customer Q&As
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16 Customer Q&As for Anti-IP10/CXCL10 Antibody Picoband®
Question
We are currently using anti-IP10/CXCL10 antibody PB9695 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
The anti-IP10/CXCL10 antibody (PB9695) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-24
Question
We have seen staining in human ectocervix. Any tips? Is anti-IP10/CXCL10 antibody supposed to stain ectocervix positively?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
Based on literature ectocervix does express CXCL10. Based on Uniprot.org, CXCL10 is expressed in ectocervix, prostate, keratinocyte, foreskin keratinocyte, among other tissues. Regarding which tissues have CXCL10 expression, here are a few articles citing expression in various tissues:
Foreskin keratinocyte, Pubmed ID: 11559369
Keratinocyte, Pubmed ID: 10233762
Prostate, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-11-20
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ectocervix using anti-IP10/CXCL10 antibody PB9695. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-09
Question
See attached the WB image, lot number and protocol we used for ectocervix using anti-IP10/CXCL10 antibody PB9695. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-29
Question
I was wanting to use your anti-IP10/CXCL10 antibody for ELISA for human ectocervix on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human ectocervix identification?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
As indicated on the product datasheet, PB9695 anti-IP10/CXCL10 antibody has been tested for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human ectocervix in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-19
Question
I see that the anti-IP10/CXCL10 antibody PB9695 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-01
Question
Is this PB9695 anti-IP10/CXCL10 antibody reactive to the isotypes of CXCL10?
D. Miller
Verified customer
Asked: 2019-05-22
Answer
The immunogen of PB9695 anti-IP10/CXCL10 antibody is E. coli-derived human IP10 recombinant protein (Position: V22-P98). Human IP10 shares 70.1% and 72.7% amino acid (aa) sequence identity with mouse and rat IP10, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-22
Question
My question regards using your anti-IP10/CXCL10 antibody for muscle organ development studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
K. Zhao
Verified customer
Asked: 2019-02-22
Answer
We appreciate your inquiry. This PB9695 anti-IP10/CXCL10 antibody is tested on hela whole cell lysate. It is guaranteed to work for ELISA, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-02-22
Question
My question regarding product PB9695, anti-IP10/CXCL10 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-01-02
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9695 anti-IP10/CXCL10 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-01-02
Question
Does PB9695 anti-IP10/CXCL10 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-10-05
Answer
As indicated on the product datasheet, PB9695 anti-IP10/CXCL10 antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-05
Question
Is there a BSA free version of anti-IP10/CXCL10 antibody PB9695 available?
Verified Customer
Verified customer
Asked: 2018-05-25
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-IP10/CXCL10 antibody PB9695 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-05-25
Question
Would anti-IP10/CXCL10 antibody PB9695 work for ELISA with ectocervix?
Verified Customer
Verified customer
Asked: 2017-11-06
Answer
According to the expression profile of ectocervix, CXCL10 is highly expressed in ectocervix. So, it is likely that anti-IP10/CXCL10 antibody PB9695 will work for ELISA with ectocervix.
Boster Scientific Support
Answered: 2017-11-06
Question
We need to test anti-IP10/CXCL10 antibody PB9695 on human ectocervix for research purposes, then I may be interested in using anti-IP10/CXCL10 antibody PB9695 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Z. Evans
Verified customer
Asked: 2017-07-05
Answer
The products we sell, including anti-IP10/CXCL10 antibody PB9695, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-07-05
Question
Is a blocking peptide available for product anti-IP10/CXCL10 antibody (PB9695)?
A. Zhang
Verified customer
Asked: 2015-06-19
Answer
We do provide the blocking peptide for product anti-IP10/CXCL10 antibody (PB9695). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-06-19
Question
We were happy with the WB result of your anti-IP10/CXCL10 antibody. However we have seen positive staining in foreskin keratinocyte secreted. using this antibody. Is that expected? Could you tell me where is CXCL10 supposed to be expressed?
D. Gonzalez
Verified customer
Asked: 2015-03-02
Answer
From literature, foreskin keratinocyte does express CXCL10. Generally CXCL10 expresses in secreted. Regarding which tissues have CXCL10 expression, here are a few articles citing expression in various tissues:
Foreskin keratinocyte, Pubmed ID: 11559369
Keratinocyte, Pubmed ID: 10233762
Prostate, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2015-03-02
Question
We purchased anti-IP10/CXCL10 antibody for WB on ectocervix a few years ago. I am using human, and We want to use the antibody for ELISA next. My lab would like examining ectocervix as well as prostate in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?
V. Jones
Verified customer
Asked: 2014-10-14
Answer
I looked at the website and datasheets of our anti-IP10/CXCL10 antibody and it appears that PB9695 has been tested on human in both WB and ELISA. Thus PB9695 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-10-14
