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Product Info Summary
| SKU: | EK0453 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human MIP-3 Alpha / CCL20 / MIP3a ELISA Kit PicoKine®
View all CCL20/MIP-3 alpha ELISA kits
SKU/Catalog Number
EK0453
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human MIP-3 Alpha / CCL20 / MIP3a ELISA Kit PicoKine® (96 Tests). Quantitate Human CCL20 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human MIP-3 Alpha / CCL20 / MIP3a ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0453)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: A27-M96
Sensitivity
<1 pg/ml
Assay Range
7.8 pg/ml - 500 pg/ml
Standard Dilution Instructions
See datasheet of EK0453 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0453 is reactive to CCL20 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0453 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CCL20/MIP-3 alpha
Macrophage Inflammatory Protein 3alpha (MIP3alpha), also called Chemokine, cc motif, ligand 20 (CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3alpha/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3alpha and Serine MIP-3alpha, the two forms of MIP3alpha, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4 (+) and CD8 (+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0453-CAP | Anti-Human CCL20 Pre-coated 96-well strip microplate | 1 |
| EK0453-ST | Human CCL20 Standard | 2 vials, 10 ng/tube |
| EK0453-DA | Human CCL20 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.053 | 0.105 | 0.171 | 0.306 | 0.478 | 0.992 | 1.467 | 2.044 |
Data Example Images
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Human MIP-3 alpha/CCL20 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CCL20/MIP-3 alpha, Dilution ratio of 1:1, concentration in serum and plasma is 0-171 pg/ml..
Some articles we found to cite concentrations of CCL20/MIP-3 alpha in samples: 18980993 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MIP-3 Alpha / CCL20 / MIP3a ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 19 | 78 | 256 | 17 | 73 | 269 |
| Standard deviation | 1.34 | 4.91 | 13.05 | 1.22 | 5.03 | 15.06 |
| CV (%) | 7.1% | 6.9% | 5.6% | 7.2% | 6.9% | 5.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CCL20/MIP-3 alpha in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 19 | 18 | 19 | 19 | 18 | 0.43 | 2.3% |
| Sample 2 | 78 | 79 | 72 | 74 | 75 | 2.86 | 3.8% |
| Sample 3 | 256 | 262 | 263 | 263 | 261 | 2.91 | 1.1% |
Protein Target Info & Infographic
Gene/Protein Information For CCL20 (Source: Uniprot.Org, NCBI)
Gene Name
CCL20
Full Name
C-C motif chemokine 20
Weight
10762 MW
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
C-C motif chemokine 20;Beta-chemokine exodus-1;CC chemokine LARC;Liver and activation-regulated chemokine;Macrophage inflammatory protein 3 alpha;MIP-3-alpha;Small-inducible cytokine A20;CCL20 (1-67);CCL20 (1-64);CCL20 (2-70);CCL20;LARC, MIP3A, SCYA20; CCL20 CKb4, Exodus, LARC, MIP-3-alpha, MIP-3a, MIP3A, SCYA20, ST38 C-C motif chemokine ligand 20 C-C motif chemokine 20|CC chemokine LARC|beta chemokine exodus-1|chemokine (C-C motif) ligand 20|liver and activation-regulated chemokine|macrophage inflammatory protein 3 alpha|small inducible cytokine subfamily A (Cys-Cys), member 20|small-inducible cytokine A20
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CCL20, check out the CCL20 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CCL20: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human MIP-3 Alpha / CCL20 / MIP3a ELISA Kit PicoKine® (EK0453)
Hello CJ!
EK0453 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Identification of genes involved in enterovirus 71 infected SK-N-SH cells
RETRACTED ARTICLE: Sodium ferulate inhibits high-fat diet-induced inflammatory factors expression in human umbilical vein endothelial cells
Altered circulating CCR6+and CXCR3+ T cell subsets are associated with poor renal prognosis in MPO-ANCA-associated vasculitis
The Expression and Regulation of Interleukin-33 in Human Epidermal Keratinocytes: A New Mediator of Atopic Dermatitis and Its Possible Signaling Pathway
Sodium ferulate inhibits high-fat diet-induced inflammatory factors expression in human umbilical vein endothelial cells
Interleukin-17 receptor D constitutes an alternative receptor for interleukin-17A important in psoriasis-like skin inflammation
Species: Human
The Expression and Regulation of Interleukin-33 in Human Epidermal Keratinocytes: A New Mediator of Atopic Dermatitis and Its Possible Signaling Pathway
Li D, Wang A, Liu X, Meisgen F, Gr??nler J, Botusan Ir, Narayanan S, Erikci E, Li X, Blomqvist L, Du L, Pivarcsi A, Sonkoly E, Chowdhury K, Catrina Sb, St??hle M, Land??n Nx. J Clin Invest. 2015 Aug 3;125(8):3008-26. Doi: 10.1172/Jci79052. Epub 20...
Calvayrac O, Rodr??guez-Calvo R, Mart??-Pamies I, Alonso J, Ferr??n B, Aguil?? S, Crespo J, Rodr??guez-Sinovas A, Rodr??guez C, Mart??nez-Gonz??lez J. J Mol Cell Cardiol. 2015 Mar;80:34-44. Doi: 10.1016/J.Yjmcc.2014.12.015. Epub 2014 Dec 20. Nor-1...
Chen D, Hu Q, Mao C, Jiao Z, Wang S, Yu L, Xu Y, Dai D, Yin L, Xu H. Cell Immunol. 2012;272(2):166-74. Doi: 10.1016/J.Cellimm.2011.10.015. Epub 2011 Oct 28. Increased Il-17-Producing Cd4(+) T Cells In Patients With Esophageal Cancer.
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
8 Customer Q&As for Human MIP-3 Alpha / CCL20 / MIP3a ELISA Kit PicoKine®
Question
Q: What is the optimal O.D. value for CCL20 ELISA kit? I performed your CCL20 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain CCL20 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-08-07
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CCL20 ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of CCL20 that can be declared/interpreted as positive by the above standard is 1pg/ml.
Boster Scientific Support
Answered: 2020-08-07
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-06-22
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-06-22
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-05-15
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-05-15
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement CCL20 using the EK0453 Human CCL20 Picokine® ELISA Kit?
N. Zhao
Verified customer
Asked: 2019-11-04
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2019-11-04
Question
Q: how to thaw whole blood sample for CCL20 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CCL20 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-07-16
Question
Q: how to analyze ELISA data? I have performed CCL20 level in plasma.
W. Phillips
Verified customer
Asked: 2018-06-14
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-06-14
Question
Q: if the enzyme conjugated CCL20 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CCL20 antigen?
Verified Customer
Verified customer
Asked: 2017-07-10
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-07-10
Question
Q: Can CCL20 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2017-01-01
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2017-01-01
