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Product Info Summary
| SKU: | EK0735 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine. |
Product info
Product Name
Human CXCL10/IP-10 ELISA Kit PicoKine®
View all CXCL10/IP-10/CRG-2 ELISA kits
SKU/Catalog Number
EK0735
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CXCL10/IP-10 ELISA Kit PicoKine® (96 Tests). Quantitate Human CXCL10 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CXCL10/IP-10 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0735)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: V22-P98
Sensitivity
<1 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0735 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0735 is reactive to CXCL10 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.
Application Guarantee
EK0735 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CXCL10/IP-10/CRG-2
Chemokine (C-X-C motif) ligand 10 (CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family that is also known as 10 kDa interferon-gamma-induced protein (gamma-IP10 or IP-10). CXCL10 is secreted by several cell types in response to IFN-gamma. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.It is a potent inhibitor of angiogenesis in vivo. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. It is a RAS target gene and is overexpressed in the majority of colorectal cancers.This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0735-CAP | Anti-Human CXCL10 Pre-coated 96-well strip microplate | 1 |
| EK0735-ST | Human CXCL10 Standard | 2 vials, 10 ng/tube |
| EK0735-DA | Human CXCL10 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.048 | 0.086 | 0.148 | 0.202 | 0.325 | 0.547 | 1.043 | 1.884 |
Data Example Images
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Human CXCL10/IP-10 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CXCL10/IP-10/CRG-2, Dilution ratio of 1:1, concentration in serum and plasma is 80-133 pg/ml..
Some articles we found to cite concentrations of CXCL10/IP-10/CRG-2 in samples: 18760981, 24120067 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CXCL10/IP-10 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 60 | 243 | 879 | 64 | 223 | 865 |
| Standard deviation | 3.24 | 19.19 | 45.7 | 4.28 | 19.4 | 47.57 |
| CV (%) | 5.4% | 7.9% | 5.2% | 6.7% | 8.7% | 5.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CXCL10/IP-10/CRG-2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 60 | 54 | 60 | 64 | 59 | 3.57 | 6% |
| Sample 2 | 243 | 258 | 258 | 260 | 254 | 6.83 | 2.6% |
| Sample 3 | 879 | 923 | 848 | 978 | 907 | 48.89 | 5.3% |
Protein Target Info & Infographic
Gene/Protein Information For CXCL10 (Source: Uniprot.Org, NCBI)
Gene Name
CXCL10
Full Name
C-X-C motif chemokine 10
Weight
10881 MW
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
C-X-C motif chemokine 10;10 kDa interferon gamma-induced protein;Gamma-IP10;IP-10;Small-inducible cytokine B10;CXCL10 (1-73);CXCL10;INP10, SCYB10; CXCL10 C7, IFI10, INP10, IP-10, SCYB10, crg-2, gIP-10, mob-1 C-X-C motif chemokine ligand 10 C-X-C motif chemokine 10|10 kDa interferon gamma-induced protein|gamma IP10|interferon-inducible cytokine IP-10|protein 10 from interferon (gamma)-induced cell line|small inducible cytokine subfamily B (Cys-X-Cys), member 10|small-inducible cytokine B10
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CXCL10, check out the CXCL10 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CXCL10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CXCL10/IP-10 ELISA Kit PicoKine® (EK0735)
Hello CJ!
EK0735 has been cited in 12 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
A correlation study between gene polymorphism of Th cell expressed chemokine receptor CXCR3 and its ligand levels with HCV infection prognosis.
A Predictive Test in The Screening of HIV Positive Cases; Interferon Gamma Inducible Protein 10 (IP-10).
Short- and Long-Term Immunological Responses in Chronic HCV/HIV Co-Infected Compared to HCV Mono-Infected Patients after DAA Therapy
The impact of Aggregatibacter actinomycetemcomitans biofilm-derived effectors following antimicrobial photodynamic therapy on cytokine production in human gingival fibroblasts
Blocking CXCR3 with AMG487 ameliorates the blood-retinal barrier disruption in diabetic mice through anti-oxidative
Distinct mucosal immunopathologic profiles in atopic and nonatopic chronic rhinosinusitis without nasal polyps in Central China
Distinct mucosal immunopathologic profiles in atopic and nonatopic chronic rhinosinusitis without nasal polyps in Central China
Distinct mucosal immunopathologic profiles in atopic and nonatopic chronic rhinosinusitis without nasal polyps in Central China
Wang Z,Ao X,Shen Z,Ao L,Wu X,Pu C,Guo W,Xing W,He M,Yuan H,Yu J,Li L,Xu X.TNF-α augments CXCL10/CXCR3 axis activity to induce Epithelial-Mesenchymal Transition in colon cancer cell.Int J Biol Sci 2021;17(11):2683-2702.doi:10.7150/ijbs.61350.Available from https://www.ijbs.com/v17p2683.htm
Species: Human
EK0735 usage in article: APP:ELISA, SAMPLE:SW480 CELL AND SW620 CELL, DILUTION:NA
Distinct mucosal immunopathologic profiles in atopic and nonatopic chronic rhinosinusitis without nasal polyps in Central China
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Human CXCL10/IP-10 ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
C. Chakrabarti
Verified customer
Asked: 2020-05-12
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-05-12
Question
Q: how to analyze ELISA data? I have performed CXCL10 level in serum.
Verified Customer
Verified customer
Asked: 2018-08-22
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-08-22
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-04-17
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-04-17
