Product Info Summary
SKU: | EZ0735 |
---|---|
Size: | For 5 plates, 96 wells each |
Reactive Species: | Human |
Application: | ELISA |
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Product info
Product Name
Human CXCL10/IP-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
See all CXCL10/IP-10/CRG-2 products
SKU/Catalog Number
EZ0735
Size
For 5 plates, 96 wells each
*Question: How many samples can I assay/run in this kit?
Description
Human CXCL10/IP-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human CXCL10 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CXCL10/IP-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0735)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: V22-P98
Assay Range
31.2 pg/ml - 2,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EZ0735 is reactive to CXCL10 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.
Application Guarantee
EZ0735 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Catalog number | Description | Quantity | Storage of opened/reconstituted material |
---|---|---|---|
EZ0735-CA | Rabbit anti- human CXCL10 polyclonal antibody (Capture Antibody) | 500 µl, 0.4 mg/mL (recommended dilution 1:100) | Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. |
EZ0735-DA | Biotinylated goat anti- human CXCL10 polyclonal antibody (Detection Antibody) | 500 µl, 10 µg/mL (recommended dilution 1:100) | |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 500 µl (recommended dilution 1:100) | |
EK0735-ST | Lyophilized recombinant human CXCL10 standard | 10 ng/tube×3 | Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Incubator.
- 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 5. Clean tubes and Eppendorf tubes.
- 6. 96 well microplate (Cat# AR1100)
- 7. Plate Sealers.
- 8. Capture Antibody Diluent: PBS.
- 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
- 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
- 11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
- 12. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. - *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.011 | 0.044 | 0.088 | 0.151 | 0.279 | 0.649 | 1.271 | 2.004 |
Data Example Images
![EZ0735 human cxcl10 ez set elisa kit diy antibody pairs EZ0735 human cxcl10 ez set elisa kit diy antibody pairs](https://www.bosterbio.com/media/catalog/product/E/Z/EZ0735-human-cxcl10-ez-set-elisa-kit-diy-antibody-pairs.jpg)
Click image to see more details
Human CXCL10/IP-10 EZ-Set ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 225 | 837 | 1469 | 273 | 996 | 1478 |
Standard deviation | 11.7 | 38.5 | 89.6 | 18.3 | 58.8 | 115.3 |
CV (%) | 5.2% | 4.6% | 6.1% | 6.7% | 5.9% | 7.8% |
Protein Target Info & Infographic
Gene/Protein Information For CXCL10 (Source: Uniprot.Org, NCBI)
Gene Name
CXCL10
Full Name
C-X-C motif chemokine 10
Weight
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10 CXCL10 C7, IFI10, INP10, IP-10, SCYB10, crg-2, gIP-10, mob-1 C-X-C motif chemokine ligand 10 C-X-C motif chemokine 10|10 kDa interferon gamma-induced protein|gamma IP10|interferon-inducible cytokine IP-10|protein 10 from interferon (gamma)-induced cell line|small inducible cytokine subfamily B (Cys-X-Cys), member 10|small-inducible cytokine B10
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CXCL10, check out the CXCL10 Infographic
![CXCL10 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CXCL10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CXCL10/IP-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ0735)
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