Product Info Summary
SKU: | M00306 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Interferon Receptor alpha IFNAR1 Rabbit Monoclonal Antibody
View all IFN-alpha/beta R1 Antibodies
SKU/Catalog Number
M00306
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Interferon Receptor alpha IFNAR1 Rabbit Monoclonal Antibody catalog # M00306. Tested in WB application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interferon Receptor alpha IFNAR1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00306)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AF-9
Isotype
Rabbit IgG
Standard Protein
A synthesized peptide derived from human Interferon Receptor alpha
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00306 is reactive to IFNAR1 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
62 kDa
Calculated molecular weight
63525 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00306 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Positive Control
WB: SH-SY5Y cell
Validation Images & Assay Conditions
![m00306 wb m00306 wb](https://www.bosterbio.com/media/catalog/product/m/0/m00306-wb.jpg)
Click image to see more details
Western blot analysis of Interferon Receptor alpha expression in SH-SY5Y cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For IFNAR1 (Source: Uniprot.org, NCBI)
Gene Name
IFNAR1
Full Name
Interferon alpha/beta receptor 1
Weight
63525 MW
Superfamily
type II cytokine receptor family
Alternative Names
alpha-type antiviral protein; AVP; beta-type antiviral protein; CRF2-1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; human interferon-alpha receptor (HuIFN-alpha-Rec)10IFRC; IFN-alpha/beta R1; IFN-alpha/beta receptor 1; IFN-alpha-REC; IFNAR; IFNAR1; IFN-aR1; IFNBR; IFNbR1; IFN-bR1; IFN-R-1; interferon (alpha, beta and omega) receptor 1; interferon alpha/beta receptor 1; interferon-alpha/beta receptor alpha chain; interferon-beta receptor 1; Type I interferon receptor 1 IFNAR1 AVP, IFN-alpha-REC, IFNAR, IFNBR, IFRC interferon alpha and beta receptor subunit 1 interferon alpha/beta receptor 1|CRF2-1|IFN-R-1|IFN-alpha/beta receptor 1|IFNalpha/beta receptor 1|alpha-type antiviral protein|beta-type antiviral protein|cytokine receptor class-II member 1|cytokine receptor family 2 member 1|interferon (alpha, beta and omega) receptor 1|interferon receptor 1|interferon-alpha/beta receptor alpha chain|interferon-beta receptor 1|type I interferon receptor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IFNAR1, check out the IFNAR1 Infographic
![IFNAR1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IFNAR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Interferon Receptor alpha IFNAR1 Rabbit Monoclonal Antibody (M00306)
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No publications found for M00306
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-Interferon Receptor alpha IFNAR1 Rabbit Monoclonal Antibody
Question
Does M00306 anti-Interferon Receptor alpha Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
As indicated on the product datasheet, M00306 anti-Interferon Receptor alpha Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-03
Question
Here is the WB image, lot number and protocol we used for liver using anti-Interferon Receptor alpha Rabbit Monoclonal antibody M00306. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-29
Question
I am interested in to test anti-Interferon Receptor alpha Rabbit Monoclonal antibody M00306 on human liver for research purposes, then I may be interested in using anti-Interferon Receptor alpha Rabbit Monoclonal antibody M00306 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
The products we sell, including anti-Interferon Receptor alpha Rabbit Monoclonal antibody M00306, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-15
Question
I was wanting to use your anti-Interferon Receptor alpha Rabbit Monoclonal antibody for WB for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
It shows on the product datasheet, M00306 anti-Interferon Receptor alpha Rabbit Monoclonal antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-12
Question
Is this M00306 anti-Interferon Receptor alpha Rabbit Monoclonal antibody reactive to the isotypes of IFNAR1?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
The immunogen of M00306 anti-Interferon Receptor alpha Rabbit Monoclonal antibody is A synthesized peptide derived from human Interferon Receptor alpha. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-13
Question
Our team were well pleased with the WB result of your anti-Interferon Receptor alpha Rabbit Monoclonal antibody. However we have been able to see positive staining in liver isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is IFNAR1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
From literature, liver does express IFNAR1. Generally IFNAR1 expresses in isoform 1: cell membrane. Regarding which tissues have IFNAR1 expression, here are a few articles citing expression in various tissues:
Brain, and Ovary, Pubmed ID: 15489334
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 10830953
Lung, Pubmed ID: 14702039
Myeloma, Pubmed ID: 8307198
Boster Scientific Support
Answered: 2019-06-13
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Interferon Receptor alpha Rabbit Monoclonal antibody M00306. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-03
Question
We have observed staining in human leukemic t-cell. Any tips? Is anti-Interferon Receptor alpha Rabbit Monoclonal antibody supposed to stain leukemic t-cell positively?
Verified Customer
Verified customer
Asked: 2019-01-23
Answer
According to literature leukemic t-cell does express IFNAR1. According to Uniprot.org, IFNAR1 is expressed in leukocyte, lung, liver, brain ovary, myeloma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have IFNAR1 expression, here are a few articles citing expression in various tissues:
Brain, and Ovary, Pubmed ID: 15489334
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 10830953
Lung, Pubmed ID: 14702039
Myeloma, Pubmed ID: 8307198
Boster Scientific Support
Answered: 2019-01-23
Question
I see that the anti-Interferon Receptor alpha Rabbit Monoclonal antibody M00306 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-11-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-11-06
Question
Can you help my question with product M00306, anti-Interferon Receptor alpha Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-09-06
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00306 anti-Interferon Receptor alpha Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-09-06
Question
Does anti-Interferon Receptor alpha Rabbit Monoclonal antibody M00306 work for WB with liver?
J. Lewis
Verified customer
Asked: 2016-04-15
Answer
According to the expression profile of liver, IFNAR1 is highly expressed in liver. So, it is likely that anti-Interferon Receptor alpha Rabbit Monoclonal antibody M00306 will work for WB with liver.
Boster Scientific Support
Answered: 2016-04-15
Question
We are currently using anti-Interferon Receptor alpha Rabbit Monoclonal antibody M00306 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
K. Moore
Verified customer
Asked: 2014-07-29
Answer
The anti-Interferon Receptor alpha Rabbit Monoclonal antibody (M00306) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-07-29
Question
Do you have a BSA free version of anti-Interferon Receptor alpha Rabbit Monoclonal antibody M00306 available?
G. Brown
Verified customer
Asked: 2014-07-02
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Interferon Receptor alpha Rabbit Monoclonal antibody M00306 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-07-02
Question
Is a blocking peptide available for product anti-Interferon Receptor alpha Rabbit Monoclonal antibody (M00306)?
L. Johnson
Verified customer
Asked: 2014-02-07
Answer
We do provide the blocking peptide for product anti-Interferon Receptor alpha Rabbit Monoclonal antibody (M00306). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-02-07