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Product Info Summary
| SKU: | M00772-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Integrin beta 1 ITGB1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00772-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Integrin beta 1 ITGB1 Rabbit Monoclonal Antibody catalog # M00772-1. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Integrin beta 1 ITGB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00772-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DDI-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Integrin beta 1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00772-1 is reactive to ITGB1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
15 kDa
Calculated molecular weight
88415 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00772-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:500
Positive Control
WB: human HT-1080 whole cell, human Hela whole cell, human U-87MG whole cell, rat liver tissue, rat C6 whole cell, mouse liver tissue
IHC: human breast cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Integrin beta 1 using anti-Integrin beta 1 antibody (M00772-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HT-1080 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin beta 1 antigen affinity purified monoclonal antibody (Catalog # M00772-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin beta 1 at approximately 140 kDa. The expected band size for Integrin beta 1 is at 88 kDa.
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Immunohistochemical analysis of paraffin-embedded human breast cancer, using Integrin beta 1 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For ITGB1 (Source: Uniprot.org, NCBI)
Gene Name
ITGB1
Full Name
Integrin beta-1
Weight
88415 MW
Superfamily
integrin beta chain family
Alternative Names
Integrin beta-1;Fibronectin receptor subunit beta;Glycoprotein IIa;GPIIA;VLA-4 subunit beta;CD29;ITGB1;FNRB, MDF2, MSK12; ITGB1 CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB integrin subunit beta 1 integrin beta-1|glycoprotein IIa|integrin VLA-4 beta subunit|integrin beta 1|integrin, beta 1 (fibronectin receptor, beta polypeptide, CD29 includes MDF2, MSK12)|very late activation protein, beta polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ITGB1, check out the ITGB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ITGB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Integrin beta 1 ITGB1 Rabbit Monoclonal Antibody (M00772-1)
Hello CJ!
M00772-1 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Abnormalities in the basement membrane structure promote basal keratinocytes in the epidermis of hypertrophic scars to adopt a proliferative phenotype
Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC-?/ERK1/2 pathway: An in vitro investigation
Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats.
Isolation and morphological characterization of ovine amniotic fluid mesenchymal stem cells
Yin D, Tian L, Ye Y, Li K, Wang J, Cheng P, Chen A, Guo F, Huang H. Int J Mol Med. 2012 Apr;29(4):587-92. Doi: 10.3892/Ijmm.2011.871. Epub 2011 Dec 29. Nanog And ??-Catenin: A New Convergence Point In Epsc Proliferation And Differentiation.
Lin H, Yang Y, Wang Y, Wang L, Zhou X, Liu J, Peng D. Plos One. 2014 Jan 21;9(1):E85672. Doi: 10.1371/Journal.Pone.0085672. Ecollection 2014. Effect Of Mixed Transplantation Of Autologous And Allogeneic Microskin Grafts On Wound Healing In A Rat M...
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