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Product Info Summary
| SKU: | A02176 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Golgin subfamily A member 5 GOLGA5 Antibody
SKU/Catalog Number
A02176
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Golgin subfamily A member 5 GOLGA5 Antibody (Catalog # A02176). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
GOLGA5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Golgin subfamily A member 5 GOLGA5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02176)
Host
Rabbit
Contents
GOLGA5 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
GOLGA5 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human GOLGA5. The immunogen is located within amino acids 540 - 590 of GOLGA5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
GOLGA5 antibody is human, mouse and rat reactive. Two isoforms of GOLGA5 exist due to alternative splicing events.
Reactive Species
A02176 is reactive to GOLGA5 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
83024 MW
Background of GOLGA5
GOLGA5 (Golgi autoantigen, subfamily A, member 5), also known as GOLGA84, RFG5 (RET-fused gene 5 protein) or GOLIM5, is a ubiquitously expressed single-pass type II coiled-coil membrane protein that is involved in maintaining Golgi structure (1). It is localized to the Golgi apparatus and predominantly found on membranes at the cis side of the Golgi stack (1,2). As a mitotic phosphoprotein, GOLGA5 binds to active Rab 1 and associates with CASP (an isoform of CDP) in a golgin-tethering complex that is believed to play a role in intra-Golgi retrograde transport (3,4). Defects in GOLGA5 are a cause of thyroid papillary carcinoma (TPC) (5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02176 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
GOLGA5 antibody can be used for detection of GOLGA5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of GOLGA5 in human testis tissue lysate with GOLGA5 antibody at 1 μg/mL.
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Immunohistochemistry of GOLGA5 in human testis tissue with GOLGA5 antibody at 5 μg/mL.
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Immunofluorescence of GOLGA5 in human testis tissue with GOLGA5 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For GOLGA5 (Source: Uniprot.org, NCBI)
Gene Name
GOLGA5
Full Name
Golgin subfamily A member 5
Weight
83024 MW
Alternative Names
RFG5, GOLIM5, ret-II, RETII, RFG5, PIG31, Golgin subfamily A member 5, Cell proliferation-inducing gene 31 protein GOLGA5 GOLIM5, RFG5, ret-II golgin A5 golgin subfamily A member 5|RET-fused gene 5 protein|cell proliferation-inducing gene 31 protein|golgi auto, golgin subfamily a, 5|golgi integral membrane protein 5|golgin-84
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GOLGA5, check out the GOLGA5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GOLGA5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Golgin subfamily A member 5 GOLGA5 Antibody (A02176)
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