Click image to see more details
Product Info Summary
| SKU: | FC00555-1 |
|---|---|
| Size: | 100ug, 500ug |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Human CD45RO PTPRC Monoclonal Antibody, Flow Validated
SKU/Catalog Number
FC00555-1
Size
100ug, 500ug
Form
Liquid, in PBS, pH7.2, <0.09% NaN3 and 0.2% (w/v) BSA
Description
Boster Bio Anti-Human CD45RO PTPRC Monoclonal Antibody Unconjugated Conjugated, Flow Validated (Catalog# FC00555-1). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human CD45RO PTPRC Monoclonal Antibody, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00555-1)
Host
Mouse
Contents
PBS pH 7.2, 0.1% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: UCHL1
Isotype
IgG2a,k
Immunogen
IL-2 dependent T cell line, CA-1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC00555-1 is reactive to PTPRC in Human
Reconstitution
Calculated molecular weight
147254 MW
Background of PTPRC
CD5, also known as T1 or LEU1, is a cluster of differentiation found on a subset of IgM-secreting B cells called B-1 cells, and also on T cells. It is mapped to 11q12.2. Although CD5 can be found on both T cells and B cells, T cells express higher levels of CD5 than B cells. CD5 was used as a T-cell marker until monoclonal antibodies against CD3 were developed. CD5 is upregulated on T cells upon strong activation. In the thymus, there is a correlation with CD5 expression and strength of the interaction of the T cell towards self-peptides. Nevertheless, CD5 serves to mitigate activating signals from the BCR so that the B-1 cells can only be activated by very strong stimuli (such as bacterial proteins) and not by normal tissue proteins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC00555-1 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
Click image to see more details
Lymphocytes gated PBMCs stained with purified anti-human CD45RO (clone UCHL1) followed by goat anti-mouse IgG (H+L) Alexa Fluor 488 (red histogram). Lymphocytes gated PBMCs stained with mouse IgG2a isotype control followed by goat anti-mouse IgG (H+L) Alexa Fluor 488 (Blue histogram). The data are generated by BD Accuri C6 Flow Cytometer and analyzed by FlowJo software.
Protein Target Info & Infographic
Gene/Protein Information For PTPRC (Source: Uniprot.org, NCBI)
Gene Name
PTPRC
Full Name
Receptor-type tyrosine-protein phosphatase C
Weight
147254 MW
Superfamily
protein-tyrosine phosphatase family
Alternative Names
Receptor-type tyrosine-protein phosphatase C;3.1.3.48;Leukocyte common antigen;L-CA;T200;CD45;PTPRC;CD45; PTPRC B220, CD45, CD45R, GP180, L-CA, LCA, LY5, T200 protein tyrosine phosphatase receptor type C receptor-type tyrosine-protein phosphatase C|CD45 |T200 glycoprotein|T200 leukocyte common |protein tyrosine phosphatase, receptor type, c polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTPRC, check out the PTPRC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTPRC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CD45RO PTPRC Monoclonal Antibody, Flow Validated (FC00555-1)
Hello CJ!
FC00555-1 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of human umbilical cord mesenchymal stem cells on endometriotic cell proliferation and apoptosis
Lipoxin A4 and its analogue suppress the tumor growth of transplanted H22 in mice: the role of antiangiogenesis
Detecting cell-in-cell structures in human tumor samples by E-cadherin/CD68/CD45 triple staining
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Human CD45RO PTPRC Monoclonal Antibody, Flow Validated?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Human CD45RO PTPRC Monoclonal Antibody, Flow Validated
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
