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Product Info Summary
| SKU: | A06903-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-IL34 Antibody Picoband®
SKU/Catalog Number
A06903-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL34 Antibody Picoband® catalog # A06903-2. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL34 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06903-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Interleukin 34/IL34, which shares 64.2% and 60.7% amino acid (aa) sequence identity with mouse and rat Interleukin 34/IL34, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06903-2 is reactive to IL34 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
27,482 MW
Background of IL-34
Interleukin 34 (IL-34) is a protein belonging to a group of cytokines called interleukins. It was originally identified in humans, by large scale screening of secreted proteins; chimpanzee, murine, rat and chicken interleukin 34 orthologs have also been found. The protein is composed of 241 amino acids, 39 kilodaltons in mass, and forms homodimers. IL-34 increases growth or survival of immune cells known as monocytes; it elicits its activity by binding the Colony stimulating factor 1 receptor. This gene is mapped to 16q22.1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06903-2 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
Positive Control
WB: human HepG2 whle cell, human A549 whole cell, human SH-SY5Y whole cell, human U87 whole cell, human CACO-2 whole cell, human U20S whole cell, human HEK293 whole cell, rat C6 whole cell,mouse ANA-1 whole cell
IHC: mhuman liver cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IL34 using anti-IL34 antibody (A06903-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whle cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates,
Lane 4: human U87 whole cell lysates,
Lane 5: human CACO-2 whole cell lysates,
Lane 6: human U20S whole cell lysates,
Lane 7: human HEK293 whole cell lysates,
Lane 8: rat C6 whole cell lysates,
Lane 9: mouse ANA-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL34 antigen affinity purified polyclonal antibody (Catalog # A06903-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL34 at approximately 39 kDa. The expected band size for IL34 is at 27 kDa.
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Figure 2. IHC analysis of IL34 using anti-IL34 antibody (A06903-2).
IL34 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL34 Antibody (A06903-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For IL34 (Source: Uniprot.org, NCBI)
Gene Name
IL34
Full Name
Interleukin-34
Weight
27,482 MW
Superfamily
IL-34 family
Alternative Names
Interleukin-34; IL-34; IL34; C16orf77 Il34|2010004A03Rik, AI593503|interleukin 34|interleukin-34
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL34, check out the IL34 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL34: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL34 Antibody Picoband® (A06903-2)
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Customer Q&As
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6 Customer Q&As for Anti-IL34 Antibody Picoband®
Question
Does A06903-2 anti-IL34 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
E. Roberts
Verified customer
Asked: 2019-12-27
Answer
You can see on the product datasheet, A06903-2 anti-IL34 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-27
Question
We are currently using anti-IL34 antibody A06903-2 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
The anti-IL34 antibody (A06903-2) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-30
Question
My lab would like to test anti-IL34 antibody A06903-2 on rat brain for research purposes, then I may be interested in using anti-IL34 antibody A06903-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-04-29
Answer
The products we sell, including anti-IL34 antibody A06903-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-04-29
Question
Is this A06903-2 anti-IL34 antibody reactive to the isotypes of IL34?
Verified Customer
Verified customer
Asked: 2018-07-09
Answer
The immunogen of A06903-2 anti-IL34 antibody is A synthetic peptide corresponding to a sequence of human Interleukin 34/IL34(NEPLEMWPLTQNEECTVTGFLRDKLQYR). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-07-09
Question
Will anti-IL34 antibody A06903-2 work on primate IHC-P with islet of langerhans?
Verified Customer
Verified customer
Asked: 2018-02-01
Answer
Our lab technicians have not validated anti-IL34 antibody A06903-2 on primate. You can run a BLAST between primate and the immunogen sequence of anti-IL34 antibody A06903-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate islet of langerhans in IHC-P, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-01
Question
Is a blocking peptide available for product anti-IL34 antibody (A06903-2)?
C. Jones
Verified customer
Asked: 2014-04-17
Answer
We do provide the blocking peptide for product anti-IL34 antibody (A06903-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-04-17
