Click image to see more details
Product Info Summary
| SKU: | A00857-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-IL27 Antibody Picoband®
SKU/Catalog Number
A00857-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL27 Antibody Picoband® catalog # A00857-2. Tested in ELISA, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL27 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00857-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse IL27 recombinant protein (Position: F29-S234).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00857-2 is reactive to Il27 in Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
29 kDa
Calculated molecular weight
27.493kDa
Background of IL27
Interleukin 27 (IL-27) is a member of the IL-12 cytokine family. The protein encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4 (+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4 (+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00857-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot,0.1-0.5μg/ml
ELISA,0.1-0.5μg/ml
Positive Control
WB: rat NRK whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of IL27 using anti-IL27 antibody (A00857-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat NRK whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL27 antigen affinity purified polyclonal antibody (Catalog # A00857-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL27 at approximately 29KD. The expected band size for IL27 is at 27KD.
Protein Target Info & Infographic
Gene/Protein Information For Il27 (Source: Uniprot.org, NCBI)
Gene Name
Il27
Full Name
Interleukin-27 subunit alpha
Weight
27.493kDa
Superfamily
IL-6 superfamily
Alternative Names
Interleukin-27 subunit alpha; IL-27 subunit alpha; IL-27-A; IL27-A; p28; Il27; Il27a IL27 IL-27, IL-27AA, IL27p28, IL30, p28, IL27 interleukin 27 interleukin-27 subunit alpha|IL-27 p28 subunit|IL-27 subunit alpha|IL-27-A|IL27-A|interleukin-30
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Il27, check out the Il27 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Il27: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL27 Antibody Picoband® (A00857-2)
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Customer Q&As
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17 Customer Q&As for Anti-IL27 Antibody Picoband®
Question
My team were satisfied with the WB result of your anti-IL27 antibody. However we have seen positive staining in lung secreted. using this antibody. Is that expected? Could you tell me where is IL27 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
From literature, lung does express IL27. Generally IL27 expresses in secreted. Regarding which tissues have IL27 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-02-13
Question
My lab would like to test anti-IL27 antibody A00857-2 on rat lung for research purposes, then I may be interested in using anti-IL27 antibody A00857-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
The products we sell, including anti-IL27 antibody A00857-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-24
Question
I have attached the WB image, lot number and protocol we used for lung using anti-IL27 antibody A00857-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-16
Question
Is there a BSA free version of anti-IL27 antibody A00857-2 available?
Verified Customer
Verified customer
Asked: 2019-04-15
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL27 antibody A00857-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-04-15
Question
Do you purify A00857-2? If so by Protein A/G or by IL-27 Ag?
Verified customer
Asked: 2019-03-04
Answer
Yes, the Anti-IL27 Antibody Picoband™ A00857-2 is purified through affinity purification by IL-27 antigen.
Boster Scientific Support
Answered: 2019-03-04
Question
Would anti-IL27 antibody A00857-2 work for ELISA with lung?
Verified Customer
Verified customer
Asked: 2018-12-03
Answer
According to the expression profile of lung, IL27 is highly expressed in lung. So, it is likely that anti-IL27 antibody A00857-2 will work for ELISA with lung.
Boster Scientific Support
Answered: 2018-12-03
Question
I am interested in using your anti-IL27 antibody for response to bacterium studies. Has this antibody been tested with western blotting on rat nrk whole cell lysates? We would like to see some validation images before ordering.
A. Li
Verified customer
Asked: 2018-09-25
Answer
Thanks for your inquiry. This A00857-2 anti-IL27 antibody is tested on rat nrk whole cell lysates. It is guaranteed to work for ELISA, WB in rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-09-25
Question
I see that the anti-IL27 antibody A00857-2 works with ELISA, what is the protocol used to produce the result images on the product page?
B. Jones
Verified customer
Asked: 2018-06-06
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-06-06
Question
Is a blocking peptide available for product anti-IL27 antibody (A00857-2)?
M. Carter
Verified customer
Asked: 2018-05-24
Answer
We do provide the blocking peptide for product anti-IL27 antibody (A00857-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-05-24
Question
We have been able to see staining in rat right lobe of liver. What should we do? Is anti-IL27 antibody supposed to stain right lobe of liver positively?
V. Kulkarni
Verified customer
Asked: 2018-04-25
Answer
From literature right lobe of liver does express IL27. From Uniprot.org, IL27 is expressed in right lobe of liver, lung, among other tissues. Regarding which tissues have IL27 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-04-25
Question
Would A00857-2 anti-IL27 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-01-19
Answer
It shows on the product datasheet, A00857-2 anti-IL27 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-01-19
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-IL27 antibody A00857-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-12-22
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-22
Question
My question regarding product A00857-2, anti-IL27 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Wu
Verified customer
Asked: 2017-02-27
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00857-2 anti-IL27 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-02-27
Question
Our lab used your anti-IL27 antibody for WB on lung a few years ago. I am using rat, and We intend to use the antibody for ELISA next. I am interested in examining lung as well as right lobe of liver in our next experiment. Could give a recommendation on which antibody would work the best for ELISA?
K. Jones
Verified customer
Asked: 2016-11-14
Answer
I have checked the website and datasheets of our anti-IL27 antibody and it appears that A00857-2 has been tested on rat in both WB and ELISA. Thus A00857-2 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-11-14
Question
Is this A00857-2 anti-IL27 antibody reactive to the isotypes of IL27?
P. Mitchell
Verified customer
Asked: 2016-05-16
Answer
The immunogen of A00857-2 anti-IL27 antibody is E. coli-derived mouse IL27 recombinant protein (Position: F29-S234). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-05-16
Question
I was wanting to use your anti-IL27 antibody for ELISA for rat lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat lung identification?
N. Kulkarni
Verified customer
Asked: 2016-02-15
Answer
It shows on the product datasheet, A00857-2 anti-IL27 antibody has been tested for ELISA, WB on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-02-15
Question
We are currently using anti-IL27 antibody A00857-2 for rat tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says rat. Is it possible that the antibody can work on feline tissues as well?
T. Taylor
Verified customer
Asked: 2013-05-08
Answer
The anti-IL27 antibody (A00857-2) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-05-08
