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Product Info Summary
| SKU: | M00963-Dyl550 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human IL22 DyLight® 550 conjugated Antibody(monoclonal, 7F2)
SKU/Catalog Number
M00963-Dyl550
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human IL22 DyLight® 550 conjugated Antibody (monoclonal, 7F2) catalog # M00963-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human IL22 DyLight® 550 conjugated Antibody(monoclonal, 7F2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00963-Dyl550)
Host
Mouse
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Monoclonal
Clone Number
7F2
Isotype
Mouse IgG1
Immunogen
E. coli-derived human IL-22 recombinant protein (Position: A34-I179). Human IL-22 shares 81.4% amino acid (aa) sequence identity with mouse IL-22.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00963-Dyl550 is reactive to IL22 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
20.011kDa
Background of IL-22
Interleukin-22 (IL-22), also known as ILTIF, is protein that in humans is encoded by the IL22 gene. IL-22 a member of a group of cytokines called the IL-10 family or IL-10 superfamily, a class of potent mediators of cellular inflammatory responses. Using FISH, the IL22 gene is mapped to chromosome 12q15, close to the IFNG and the herpesvirus saimiri-induced AK155 genes. IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. It also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10. In some contexts, the pro-inflammatory versus tissue-protective functions of IL-22 are regulated by the often co-expressed cytokine IL-17A.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00963-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Validation Images & Assay Conditions
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Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For IL22 (Source: Uniprot.org, NCBI)
Gene Name
IL22
Full Name
Interleukin-22
Weight
20.011kDa
Superfamily
IL-10 family
Alternative Names
Interleukin-22; IL-22; Cytokine Zcyto18; IL-10-related T-cell-derived-inducible factor; IL-TIF; IL22; ILTIF, ZCYTO18; UNQ3099/PRO10096 IL22 IL-21, IL-22, IL-D110, IL-TIF, ILTIF, TIFIL-23, TIFa, zcyto18 interleukin 22 interleukin-22|IL-10-related T-cell-derived inducible factor|cytokine Zcyto18
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL22, check out the IL22 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL22: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human IL22 DyLight® 550 conjugated Antibody(monoclonal, 7F2) (M00963-Dyl550)
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-Human IL22 DyLight® 550 conjugated Antibody(monoclonal, 7F2)
Question
Is a blocking peptide available for product anti-Human IL22 DyLight® 550 conjugated antibody(monoclonal, 7F2) (M00963-Dyl550)?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
We do provide the blocking peptide for product anti-Human IL22 DyLight® 550 conjugated antibody(monoclonal, 7F2) (M00963-Dyl550). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-27
Question
Does anti-Human IL22 DyLight® 550 conjugated antibody(monoclonal, 7F2) M00963-Dyl550 work for Flow Cytometry with zone of skin?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
According to the expression profile of zone of skin, IL22 is highly expressed in zone of skin. So, it is likely that anti-Human IL22 DyLight® 550 conjugated antibody(monoclonal, 7F2) M00963-Dyl550 will work for Flow Cytometry with zone of skin.
Boster Scientific Support
Answered: 2020-02-25
Question
We have seen staining in human zone of skin. Do you have any suggestions? Is anti-Human IL22 DyLight® 550 conjugated antibody(monoclonal, 7F2) supposed to stain zone of skin positively?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
According to literature zone of skin does express IL22. According to Uniprot.org, IL22 is expressed in zone of skin, blood, among other tissues. Regarding which tissues have IL22 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-02-21
Question
We were well pleased with the WB result of your anti-Human IL22 DyLight® 550 conjugated antibody(monoclonal, 7F2). However we have been able to see positive staining in zone of skin secreted. using this antibody. Is that expected? Could you tell me where is IL22 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
From what I have seen in literature, zone of skin does express IL22. Generally IL22 expresses in secreted. Regarding which tissues have IL22 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-02-04
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for zone of skin using anti-Human IL22 DyLight® 550 conjugated antibody(monoclonal, 7F2) M00963-Dyl550. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-24
Question
See attached the WB image, lot number and protocol we used for zone of skin using anti-Human IL22 DyLight® 550 conjugated antibody(monoclonal, 7F2) M00963-Dyl550. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-16
Question
Can you help my question with product M00963-Dyl550, anti-Human IL22 DyLight® 550 conjugated antibody(monoclonal, 7F2). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00963-Dyl550 anti-Human IL22 DyLight® 550 conjugated antibody(monoclonal, 7F2), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-11
Question
We are currently using anti-Human IL22 DyLight® 550 conjugated antibody(monoclonal, 7F2) M00963-Dyl550 for human tissue, and we are well pleased with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
L. Mangal
Verified customer
Asked: 2019-10-25
Answer
The anti-Human IL22 DyLight® 550 conjugated antibody(monoclonal, 7F2) (M00963-Dyl550) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-25
Question
Will M00963-Dyl550 anti-Human IL22 DyLight® 550 conjugated antibody(monoclonal, 7F2) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
You can see on the product datasheet, M00963-Dyl550 anti-Human IL22 DyLight® 550 conjugated antibody(monoclonal, 7F2) as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-03
Question
Do you have a BSA free version of anti-Human IL22 DyLight® 550 conjugated antibody(monoclonal, 7F2) M00963-Dyl550 available?
Verified Customer
Verified customer
Asked: 2018-11-29
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human IL22 DyLight® 550 conjugated antibody(monoclonal, 7F2) M00963-Dyl550 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-11-29
Question
I was wanting to use your anti-Human IL22 DyLight® 550 conjugated antibody(monoclonal, 7F2) for Flow Cytometry for human zone of skin on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human zone of skin identification?
Verified Customer
Verified customer
Asked: 2017-10-17
Answer
As indicated on the product datasheet, M00963-Dyl550 anti-Human IL22 DyLight® 550 conjugated antibody(monoclonal, 7F2) has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human zone of skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-17
Question
I am looking for to test anti-Human IL22 DyLight® 550 conjugated antibody(monoclonal, 7F2) M00963-Dyl550 on human zone of skin for research purposes, then I may be interested in using anti-Human IL22 DyLight® 550 conjugated antibody(monoclonal, 7F2) M00963-Dyl550 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-10-12
Answer
The products we sell, including anti-Human IL22 DyLight® 550 conjugated antibody(monoclonal, 7F2) M00963-Dyl550, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-12
Question
Is this M00963-Dyl550 anti-Human IL22 DyLight® 550 conjugated antibody(monoclonal, 7F2) reactive to the isotypes of IL22?
S. Edwards
Verified customer
Asked: 2013-10-29
Answer
The immunogen of M00963-Dyl550 anti-Human IL22 DyLight® 550 conjugated antibody(monoclonal, 7F2) is E. coli-derived human IL-22 recombinant protein (Position: A34-I179). Human IL-22 shares 81.4% amino acid (aa) sequence identity with mouse IL-22. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-10-29
Question
I see that the anti-Human IL22 DyLight® 550 conjugated antibody(monoclonal, 7F2) M00963-Dyl550 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
H. Williams
Verified customer
Asked: 2013-08-12
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-08-12
