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Product Info Summary
| SKU: | PROTQ8NEV9 |
|---|---|
| Size: | 1ug, 5ug, 100ug |
| Origin Species: | Human |
| Source: | HEK |
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Product info
Product Name
IL-27 Interleukin-27 Human Recombinant Protein
View all IL27 recombinant proteins
SKU/Catalog Number
PROTQ8NEV9
Size
1ug, 5ug, 100ug
Description
IL-27 Human Recombinant produced in HEK cells is a glycosylated non-covalently heterodimer that consists of 2 chains, IL27A (IL27p28) having a Mw of 25kDa and IL27B (EBI3) having a Mw of 30kDa. Each chain migrates separately on SDS-PAGE. The IL-27 protein is purified by proprietary chromatographic techniques.
Storage & Handling
Lyophilized IL-27 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL27 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Cite This Product
IL-27 Interleukin-27 Human Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ8NEV9)
Form
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The IL-27 was lyophilized from 1mg/ml in 1xPBS.
Purity
Greater than 90% as obsereved by SDS-PAGE.
Predicted MW
27.493kDa
Reconstitution
It is recommended to reconstitute the lyophilized IL-27 in sterile PBS containing 0.1% endotoxin-free recombinant HSA.
Amino Acid Sequence
IL-27A (215aa): FPRPPGRPQL SLQELRREFT VSLHLARKLL AEVRGQAHRF AESHLPGVNL YLLPLGEQLP DVSLTFQAWR RLSDPERLCF ISTTLQPFHA LLGGLGTQGR WTNMERMQLW AMRLDLRDLQ RHLRFQVLAA GFNLPEEEEE EEEEEEEERK GLLPGALGSA LQGPAQVSWP QLLSTYRLLH SLELVLSRAV RELLLLSKAG HSVWPLGFPT LSPQP. IL-27B (209aa): RKGPPAALTL PRVQCRASRY PIAVDCSWTL PPAPNSTSPV SFIATYRLGM AARGHSWPCL QQTPTSTSCT ITDVQLFSMA PYVLNVTAVH PWGSSSSFVP FITEHIIKPD PPEGVRLSPL AERQLQVQWE PPGSWPFPEI FSLKYWIRYK RQGAARFHRV GPIEATSFIL RAVRPRARYY VQVAAQDLTD YGELSDWSLP ATATMSLGK
Biological Activity
The specific activity was determined by the dose dependent inhibition of HIV replication in human CD4+ T cells and in human monocyte-derived macrophages and is typically 4-12ng/ml.
Assay dilution & Images
Reconstitution
It is recommended to reconstitute the lyophilized IL-27 in sterile PBS containing 0.1% endotoxin-free recombinant HSA.
Validation Images & Assay Conditions
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Recombinant protein fun image
Protein Target Info & Infographic
Gene/Protein Information For IL27 (Source: Uniprot.org, NCBI)
Gene Name
IL27
Full Name
Interleukin-27 subunit alpha
Weight
27.493kDa
Superfamily
IL-6 superfamily
Alternative Names
Interleukin-30; IL-30; IL-27/p28; p28; Interleukin-27; Interleukin-27/p28; IL-27; Interleukin-27 subunit alpha; IL-27 subunit alpha; IL27-A; Il27; Il27a; IL-27p28 IL27 IL-27, IL-27AA, IL27p28, IL30, p28, IL27 interleukin 27 interleukin-27 subunit alpha|IL-27 p28 subunit|IL-27 subunit alpha|IL-27-A|IL27-A|interleukin-30
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on IL27, check out the IL27 Infographic
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In this infographic, you will see the following information for IL27: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For IL-27 Interleukin-27 Human Recombinant Protein (PROTQ8NEV9)
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