Anti-IL-8 CXCL8 Antibody

CXCL8/IL-8 antibody

Boster Bio Anti-IL-8 CXCL8 Antibody (Catalog # A00423). Tested in ELISA, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A00423
Size: 1mL
Reactive Species: Human
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-IL-8 CXCL8 Antibody

View all CXCL8/IL-8 Antibodies

SKU/Catalog Number

A00423

Size

1mL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-IL-8 CXCL8 Antibody (Catalog # A00423). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store anti-IL8 antibody at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-IL-8 CXCL8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00423)

Host

Rabbit

Contents

None

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

The whole rabbit serum was prepared by repeated immunizations with recombinant human IL-8 produced in E.coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00423 is reactive to IL8 in Human

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

11098 MW

Background of CXCL8/IL-8

Interleukin-8 is a chemokine produced by macrophages and other cell types such as epithelial cells. Human IL-8 (CXCL8) antibody is commonly used in many experiments focused around inflammation. Anti-IL-8 antibody is ideal for investigators involved in Cancer, Immunology and Cardiovascular research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A00423 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:1,000 - 1:5,000
IHC: 1:400 - 1:800
IP: 1:400 - 1:800
WB: 1:500 - 1:2,000

Validation Images & Assay Conditions

Gene/Protein Information For IL8 (Source: Uniprot.org, NCBI)

Gene Name

IL8

Full Name

Interleukin-8

Weight

11098 MW

Superfamily

intercrine alpha (chemokine CxC) family

Alternative Names

Anti-CXCL 8, CXCL8, 310C, AMCF 1, AMCF1, b ENAP, Beta thromboglobulin like protein, CXC chemokine ligand 8, interleukin8, K60, LECT, LUCT, Interleukin 8, IL-8 CXCL8 GCP-1, GCP1, IL8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8 C-X-C motif chemokine ligand 8 interleukin-8|T-cell chemotactic factor|alveolar macrophage chemotactic factor I|beta endothelial cell-derived neutrophil activating peptide|beta-thromboglobulin-like protein|chemokine (C-X-C motif) ligand 8|emoctakin|granulocyte chemotactic protein 1|interleukin 8|lung giant cell carcinoma-derived chemotactic protein|lymphocyte derived neutrophil activating peptide|monocyte-derived neutrophil chemotactic factor|monocyte-derived neutrophil-activating peptide|neutrophil-activating peptide 1|small inducible cytokine subfamily B, member 8|tumor necrosis factor-induced gene 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on IL8, check out the IL8 Infographic

IL8 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for IL8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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14 Customer Q&As for Anti-IL-8 CXCL8 Antibody

Question

We are currently using anti-IL-8 antibody A00423 for human tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-10

Answer

The anti-IL-8 antibody (A00423) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-10

Question

I was wanting to use your anti-IL-8 antibody for WB for human chronic myeloid leukemia cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human chronic myeloid leukemia cell identification?

Verified Customer

Verified customer

Asked: 2019-10-10

Answer

You can see on the product datasheet, A00423 anti-IL-8 antibody has been tested for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human chronic myeloid leukemia cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-10

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for chronic myeloid leukemia cell using anti-IL-8 antibody A00423. Let me know if you need anything else.

S. Evans

Verified customer

Asked: 2019-10-07

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-07

Question

Our lab used your anti-IL-8 antibody for IP on peripheral blood leukocyte a few months ago. I am using human, and We want to use the antibody for WB next. I am looking for examining peripheral blood leukocyte as well as skin in our next experiment. Do you have any suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-09-10

Answer

I have checked the website and datasheets of our anti-IL-8 antibody and it seems that A00423 has been tested on human in both IP and WB. Thus A00423 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-09-10

Question

I am interested in to test anti-IL-8 antibody A00423 on human chronic myeloid leukemia cell for research purposes, then I may be interested in using anti-IL-8 antibody A00423 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-08-20

Answer

The products we sell, including anti-IL-8 antibody A00423, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-08-20

Question

We have seen staining in human skin. Do you have any suggestions? Is anti-IL-8 antibody supposed to stain skin positively?

Verified Customer

Verified customer

Asked: 2019-07-01

Answer

From literature skin does express CXCL8. From Uniprot.org, CXCL8 is expressed in periodontal ligament, lung carcinoma, neutrophil, lung, peripheral blood leukocyte, kidney, skin, chronic myeloid leukemia cell, among other tissues. Regarding which tissues have CXCL8 expression, here are a few articles citing expression in various tissues:
Chronic myeloid leukemia cell, Pubmed ID: 8344717
Kidney, Pubmed ID: 2523801
Lung, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 2200751
Neutrophil, Pubmed ID: 14702039
Skin, Pubmed ID: 1755384

Boster Scientific Support

Answered: 2019-07-01

Question

Is a blocking peptide available for product anti-IL-8 antibody (A00423)?

Verified Customer

Verified customer

Asked: 2018-08-15

Answer

We do provide the blocking peptide for product anti-IL-8 antibody (A00423). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-08-15

Question

Does anti-IL-8 antibody A00423 work for WB with chronic myeloid leukemia cell?

Verified Customer

Verified customer

Asked: 2018-04-19

Answer

According to the expression profile of chronic myeloid leukemia cell, CXCL8 is highly expressed in chronic myeloid leukemia cell. So, it is likely that anti-IL-8 antibody A00423 will work for WB with chronic myeloid leukemia cell.

Boster Scientific Support

Answered: 2018-04-19

Question

My question regarding product A00423, anti-IL-8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

T. Roberts

Verified customer

Asked: 2017-03-22

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00423 anti-IL-8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-03-22

Question

I see that the anti-IL-8 antibody A00423 works with WB, what is the protocol used to produce the result images on the product page?

Z. Wu

Verified customer

Asked: 2016-02-23

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2016-02-23

Question

See attached the WB image, lot number and protocol we used for chronic myeloid leukemia cell using anti-IL-8 antibody A00423. Please let me know if you require anything else.

T. Krishna

Verified customer

Asked: 2015-01-06

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-01-06

Question

Does A00423 anti-IL-8 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Z. Wu

Verified customer

Asked: 2014-11-11

Answer

As indicated on the product datasheet, A00423 anti-IL-8 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-11-11

Question

Is there a BSA free version of anti-IL-8 antibody A00423 available?

A. Anderson

Verified customer

Asked: 2013-11-01

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL-8 antibody A00423 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-11-01

Question

My boss were content with the WB result of your anti-IL-8 antibody. However we have observed positive staining in lung carcinoma secreted. using this antibody. Is that expected? Could you tell me where is CXCL8 supposed to be expressed?

R. Jackson

Verified customer

Asked: 2013-02-13

Answer

Based on literature, lung carcinoma does express CXCL8. Generally CXCL8 expresses in secreted. Regarding which tissues have CXCL8 expression, here are a few articles citing expression in various tissues:
Chronic myeloid leukemia cell, Pubmed ID: 8344717
Kidney, Pubmed ID: 2523801
Lung, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 2200751
Neutrophil, Pubmed ID: 14702039
Skin, Pubmed ID: 1755384

Boster Scientific Support

Answered: 2013-02-13

Order DetailsPrice
A00423-1 mL

1 mL

$1628

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A00423
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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