Product Info Summary
SKU: | A00423 |
---|---|
Size: | 1mL |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-IL-8 CXCL8 Antibody
View all CXCL8/IL-8 Antibodies
SKU/Catalog Number
A00423
Size
1mL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-IL-8 CXCL8 Antibody (Catalog # A00423). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store anti-IL8 antibody at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-IL-8 CXCL8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00423)
Host
Rabbit
Contents
None
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
The whole rabbit serum was prepared by repeated immunizations with recombinant human IL-8 produced in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00423 is reactive to IL8 in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
11098 MW
Background of CXCL8/IL-8
Interleukin-8 is a chemokine produced by macrophages and other cell types such as epithelial cells. Human IL-8 (CXCL8) antibody is commonly used in many experiments focused around inflammation. Anti-IL-8 antibody is ideal for investigators involved in Cancer, Immunology and Cardiovascular research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00423 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:1,000 - 1:5,000
IHC: 1:400 - 1:800
IP: 1:400 - 1:800
WB: 1:500 - 1:2,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of IL-8 expression in Recombinant Human IL-8 50ng (lane 1). IL-8 at 11KD was detected using rabbit anti-IL-8 Antigen Affinity purified polyclonal antibody (Catalog # A00423) at 1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info & Infographic
Gene/Protein Information For IL8 (Source: Uniprot.org, NCBI)
Gene Name
IL8
Full Name
Interleukin-8
Weight
11098 MW
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
Anti-CXCL 8, CXCL8, 310C, AMCF 1, AMCF1, b ENAP, Beta thromboglobulin like protein, CXC chemokine ligand 8, interleukin8, K60, LECT, LUCT, Interleukin 8, IL-8 CXCL8 GCP-1, GCP1, IL8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8 C-X-C motif chemokine ligand 8 interleukin-8|T-cell chemotactic factor|alveolar macrophage chemotactic factor I|beta endothelial cell-derived neutrophil activating peptide|beta-thromboglobulin-like protein|chemokine (C-X-C motif) ligand 8|emoctakin|granulocyte chemotactic protein 1|interleukin 8|lung giant cell carcinoma-derived chemotactic protein|lymphocyte derived neutrophil activating peptide|monocyte-derived neutrophil chemotactic factor|monocyte-derived neutrophil-activating peptide|neutrophil-activating peptide 1|small inducible cytokine subfamily B, member 8|tumor necrosis factor-induced gene 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL8, check out the IL8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL-8 CXCL8 Antibody (A00423)
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No publications found for A00423
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Customer Q&As
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14 Customer Q&As for Anti-IL-8 CXCL8 Antibody
Question
We are currently using anti-IL-8 antibody A00423 for human tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
The anti-IL-8 antibody (A00423) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-10
Question
I was wanting to use your anti-IL-8 antibody for WB for human chronic myeloid leukemia cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human chronic myeloid leukemia cell identification?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
You can see on the product datasheet, A00423 anti-IL-8 antibody has been tested for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human chronic myeloid leukemia cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-10
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for chronic myeloid leukemia cell using anti-IL-8 antibody A00423. Let me know if you need anything else.
S. Evans
Verified customer
Asked: 2019-10-07
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-07
Question
Our lab used your anti-IL-8 antibody for IP on peripheral blood leukocyte a few months ago. I am using human, and We want to use the antibody for WB next. I am looking for examining peripheral blood leukocyte as well as skin in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
I have checked the website and datasheets of our anti-IL-8 antibody and it seems that A00423 has been tested on human in both IP and WB. Thus A00423 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-09-10
Question
I am interested in to test anti-IL-8 antibody A00423 on human chronic myeloid leukemia cell for research purposes, then I may be interested in using anti-IL-8 antibody A00423 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
The products we sell, including anti-IL-8 antibody A00423, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-20
Question
We have seen staining in human skin. Do you have any suggestions? Is anti-IL-8 antibody supposed to stain skin positively?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
From literature skin does express CXCL8. From Uniprot.org, CXCL8 is expressed in periodontal ligament, lung carcinoma, neutrophil, lung, peripheral blood leukocyte, kidney, skin, chronic myeloid leukemia cell, among other tissues. Regarding which tissues have CXCL8 expression, here are a few articles citing expression in various tissues:
Chronic myeloid leukemia cell, Pubmed ID: 8344717
Kidney, Pubmed ID: 2523801
Lung, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 2200751
Neutrophil, Pubmed ID: 14702039
Skin, Pubmed ID: 1755384
Boster Scientific Support
Answered: 2019-07-01
Question
Is a blocking peptide available for product anti-IL-8 antibody (A00423)?
Verified Customer
Verified customer
Asked: 2018-08-15
Answer
We do provide the blocking peptide for product anti-IL-8 antibody (A00423). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-08-15
Question
Does anti-IL-8 antibody A00423 work for WB with chronic myeloid leukemia cell?
Verified Customer
Verified customer
Asked: 2018-04-19
Answer
According to the expression profile of chronic myeloid leukemia cell, CXCL8 is highly expressed in chronic myeloid leukemia cell. So, it is likely that anti-IL-8 antibody A00423 will work for WB with chronic myeloid leukemia cell.
Boster Scientific Support
Answered: 2018-04-19
Question
My question regarding product A00423, anti-IL-8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
T. Roberts
Verified customer
Asked: 2017-03-22
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00423 anti-IL-8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-03-22
Question
I see that the anti-IL-8 antibody A00423 works with WB, what is the protocol used to produce the result images on the product page?
Z. Wu
Verified customer
Asked: 2016-02-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-02-23
Question
See attached the WB image, lot number and protocol we used for chronic myeloid leukemia cell using anti-IL-8 antibody A00423. Please let me know if you require anything else.
T. Krishna
Verified customer
Asked: 2015-01-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-01-06
Question
Does A00423 anti-IL-8 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Z. Wu
Verified customer
Asked: 2014-11-11
Answer
As indicated on the product datasheet, A00423 anti-IL-8 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-11-11
Question
Is there a BSA free version of anti-IL-8 antibody A00423 available?
A. Anderson
Verified customer
Asked: 2013-11-01
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL-8 antibody A00423 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-11-01
Question
My boss were content with the WB result of your anti-IL-8 antibody. However we have observed positive staining in lung carcinoma secreted. using this antibody. Is that expected? Could you tell me where is CXCL8 supposed to be expressed?
R. Jackson
Verified customer
Asked: 2013-02-13
Answer
Based on literature, lung carcinoma does express CXCL8. Generally CXCL8 expresses in secreted. Regarding which tissues have CXCL8 expression, here are a few articles citing expression in various tissues:
Chronic myeloid leukemia cell, Pubmed ID: 8344717
Kidney, Pubmed ID: 2523801
Lung, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 2200751
Neutrophil, Pubmed ID: 14702039
Skin, Pubmed ID: 1755384
Boster Scientific Support
Answered: 2013-02-13