Click image to see more details
Product Info Summary
| SKU: | A00755 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CCR4 Antibody Picoband®
SKU/Catalog Number
A00755
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CCR4 Antibody Picoband® catalog # A00755. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CCR4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00755)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CCR4, which shares 93.3% amino acid (aa) sequence identity with both mouse and rat CCR4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00755 is reactive to CCR4 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
41 kDa
Calculated molecular weight
73243 MW
Background of CCR4
CCR4, C-C chemokine receptor type 4 is a protein that in humans is encoded by the CCR4 gene. CCR4 has also recently been designated CD194 (cluster of differentiation 194). The protein encoded by this gene belongs to the G protein-coupled receptor family. It is a receptor for the following CC chemokines. The CCR4 gene is mapped to chromosome 3. Chemokines are a group of small structurally related proteins that regulate cell trafficking of various types of leukocytes. The chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00755 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot,0.1-0.5μg/ml
Positive Control
WB: rat thymus tissue, mouse thymus tissue, mouse NIH3T3 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CCR4 using anti-CCR4 antibody (A00755).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: mouse thymus tissue lysates,
Lane 3: mouse NIH3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR4 antigen affinity purified polyclonal antibody (Catalog # A00755) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR4 at approximately 41KD. The expected band size for CCR4 is at 41KD.
Protein Target Info & Infographic
Gene/Protein Information For CCR4 (Source: Uniprot.org, NCBI)
Gene Name
CCR4
Full Name
C-C chemokine receptor type 4
Weight
73243 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
C-C chemokine receptor type 4; C-C CKR-4; CC-CKR-4; CCR-4; CCR4; K5-5; CD194; CCR4; CMKBR4 CCR4 CC-CKR-4, CD194, CKR4, CMKBR4, ChemR13, HGCN:14099, K5-5 C-C motif chemokine receptor 4 C-C chemokine receptor type 4|C-C CKR-4|CCR-4|chemokine (C-C motif) receptor 4|chemokine (C-C) receptor 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCR4, check out the CCR4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCR4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CCR4 Antibody Picoband® (A00755)
Hello CJ!
A00755 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhao C, Bu X, Wang W, Ma T, Ma H. Plos One. 2014 Jan 8;9(1):E85058. Doi: 10.1371/Journal.Pone.0085058. Ecollection 2014. Gec-Derived Sfrp5 Inhibits Wnt5A-Induced Macrophage Chemotaxis And Activation.
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-CCR4 Antibody Picoband®
Question
We have seen staining in rat leukocyte. Are there any suggestions? Is anti-CCR4 antibody supposed to stain leukocyte positively?
Z. Johnson
Verified customer
Asked: 2020-03-16
Answer
Based on literature leukocyte does express CCR4. Based on Uniprot.org, CCR4 is expressed in leukocyte, spleen, blood, among other tissues. Regarding which tissues have CCR4 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Spleen, Pubmed ID: 7642634
Boster Scientific Support
Answered: 2020-03-16
Question
Does anti-CCR4 antibody A00755 work for WB with blood?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
According to the expression profile of blood, CCR4 is highly expressed in blood. So, it is likely that anti-CCR4 antibody A00755 will work for WB with blood.
Boster Scientific Support
Answered: 2020-01-02
Question
Our team were happy with the WB result of your anti-CCR4 antibody. However we have seen positive staining in leukocyte cell membrane using this antibody. Is that expected? Could you tell me where is CCR4 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
From literature, leukocyte does express CCR4. Generally CCR4 expresses in cell membrane. Regarding which tissues have CCR4 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Spleen, Pubmed ID: 7642634
Boster Scientific Support
Answered: 2019-11-11
Question
I see that the anti-CCR4 antibody A00755 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-29
Question
We need to test anti-CCR4 antibody A00755 on human blood for research purposes, then I may be interested in using anti-CCR4 antibody A00755 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
The products we sell, including anti-CCR4 antibody A00755, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-30
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-CCR4 antibody A00755. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-13
Question
We are currently using anti-CCR4 antibody A00755 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
The anti-CCR4 antibody (A00755) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-17
Question
I have a question about product A00755, anti-CCR4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
H. Brown
Verified customer
Asked: 2019-05-13
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00755 anti-CCR4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-13
Question
I was wanting to use your anti-CCR4 antibody for WB for human blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human blood identification?
M. Kulkarni
Verified customer
Asked: 2019-04-03
Answer
It shows on the product datasheet, A00755 anti-CCR4 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-03
Question
Does A00755 anti-CCR4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-03-07
Answer
You can see on the product datasheet, A00755 anti-CCR4 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-03-07
Question
I have attached the WB image, lot number and protocol we used for blood using anti-CCR4 antibody A00755. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-09-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-26
Question
Is there a BSA free version of anti-CCR4 antibody A00755 available?
Verified Customer
Verified customer
Asked: 2017-12-27
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CCR4 antibody A00755 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-12-27
Question
Is a blocking peptide available for product anti-CCR4 antibody (A00755)?
Verified Customer
Verified customer
Asked: 2017-09-08
Answer
We do provide the blocking peptide for product anti-CCR4 antibody (A00755). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-09-08
Question
My question regards using your anti-CCR4 antibody for tolerance induction studies. Has this antibody been tested with western blotting on nih3t3 whole cell lysates? We would like to see some validation images before ordering.
T. Rodriguez
Verified customer
Asked: 2014-07-04
Answer
I appreciate your inquiry. This A00755 anti-CCR4 antibody is validated on rat thymus tissue, mouse thymus tissue, nih3t3 whole cell lysates. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-07-04
Question
Is this A00755 anti-CCR4 antibody reactive to the isotypes of CCR4?
D. Krishna
Verified customer
Asked: 2013-10-02
Answer
The immunogen of A00755 anti-CCR4 antibody is A synthetic peptide corresponding to a sequence of human CCR4 (LVELEVLQDCTFERYLDYAIQATETLAFVH). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-10-02
