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Product Info Summary
| SKU: | PA1353 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-IL8/CXCL8 Antibody Picoband®
View all CXCL8/IL-8 Antibodies
SKU/Catalog Number
PA1353
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL8/CXCL8 Antibody catalog # PA1353. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL8/CXCL8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1353)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human IL8.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1353 is reactive to CXCL8 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
11 kDa
Calculated molecular weight
11098 MW
Background of CXCL8/IL-8
Interleukin-8 (IL-8) is a chemokine produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles, the Weibel-Palade bodies. In humans, the interleukin-8 protein is encoded by the IL8 gene. Interleukin-8 (IL8) is a member of the CXC chemokine family (Hull et al., 2001). IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. The genes for IL8 have been co-localized on a 335-kb genomic fragment.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1353 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
Positive Control
IHC: human stomach cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IL8 using anti-IL8 antibody (PA1353).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human IL8 protein 10ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL8 antigen affinity purified polyclonal antibody (Catalog # PA1353) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GH1 at approximately 11 kDa.
Click image to see more details
Figure 2. IHC analysis of IL8 using anti-IL8 antibody (PA1353).
IL8 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL8 Antibody (PA1353) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CXCL8 (Source: Uniprot.org, NCBI)
Gene Name
CXCL8
Full Name
Interleukin-8
Weight
11098 MW
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
Interleukin-8;IL-8;C-X-C motif chemokine 8;Chemokine (C-X-C motif) ligand 8;Emoctakin;Granulocyte chemotactic protein 1;GCP-1;Monocyte-derived neutrophil chemotactic factor;MDNCF;Monocyte-derived neutrophil-activating peptide;MONAP;Neutrophil-activating protein 1;NAP-1;Protein 3-10C;T-cell chemotactic factor;MDNCF-a;GCP/IL-8 protein IV;IL8/NAP1 form I;Interleukin-8; (Ala-IL-8)77;GCP/IL-8 protein II;IL-8 (1-77);IL8/NAP1 form II;MDNCF-b;IL-8 (5-77);IL-8 (6-77); (Ser-IL-8)72;GCP/IL-8 protein I;IL8/NAP1 form III;Lymphocyte-derived neutrophil-activating factor;LYNAP;MDNCF-c;Neutrophil-activating factor;NAF;IL-8 (7-77);GCP/IL-8 protein V;IL8/NAP1 form IV;IL-8 (8-77);GCP/IL-8 protein VI;IL8/NAP1 form V;IL-8 (9-77);GCP/IL-8 protein III;IL8/NAP1 form VI;CXCL8;IL8; CXCL8 GCP-1, GCP1, IL8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8 C-X-C motif chemokine ligand 8 interleukin-8|T-cell chemotactic factor|alveolar macrophage chemotactic factor I|beta endothelial cell-derived neutrophil activating peptide|beta-thromboglobulin-like protein|chemokine (C-X-C motif) ligand 8|emoctakin|granulocyte chemotactic protein 1|interleukin 8|lung giant cell carcinoma-derived chemotactic protein|lymphocyte derived neutrophil activating peptide|monocyte-derived neutrophil chemotactic factor|monocyte-derived neutrophil-activating peptide|neutrophil-activating peptide 1|small inducible cytokine subfamily B, member 8|tumor necrosis factor-induced gene 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CXCL8, check out the CXCL8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CXCL8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL8/CXCL8 Antibody Picoband® (PA1353)
Hello CJ!
PA1353 has been cited in 46 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Inhibition of endoplasmic reticulum stress signaling pathway: A new mechanism of statins to suppress the development of abdominal aortic aneurysm
Ginsenoside Rg3 inhibits the senescence of prostate stromal cells through down-regulation of interleukin 8 expression
FOXQ1 regulates senescence-associated inflammation via activation of SIRT1 expression
C/EBP ? Mediates Endoplasmic Reticulum Stress Regulated Inflammatory Response and Extracellular Matrix Degradation in LPS-Stimulated Human Periodontal Ligament Cells
Inhibition of zymosan-induced cytokine and chemokine expression in human corneal fibroblasts by triptolide
Inflammatory response of macrophages cultured with Helicobacter pylori strains was regulated by miR-155
Evaluation of the Inflammatory Response in Macrophages Stimulated with Exosomes Secreted by?Mycobacterium avium-Infected Macrophages
Neuroprotective effect of pretreatment with ganoderma lucidum in cerebral ischemia/reperfusion injury in rat hippocampus
Jia YP, Wang K, Zhang ZJ, Tong YN, Han D, Hu CY, Li Q, Xiang Y, Mao XH, Tang B. PLoS One. 2017 Oct 9;12(10):e0186179. doi: 10.1371/journal.pone.0186179. eCollection 2017. TLR2/TLR4 activation induces Tregs and suppresses intestinal inflammation ca...
Wang B, Li K, Jin W, Lu Y, Zhang Y, Shen G, Wang R, Shen H, Li W, Huang Y, Zhang Y, Wang X, Li X, Liu W, Cao H, Tao S. Environ Sci Technol. 2013 Sep 17;47(18):10583-90. Doi: 10.1021/Es401394G. Epub 2013 Sep 4. Properties And Inflammatory Effects O...
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Customer Q&As
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5 Customer Q&As for Anti-IL8/CXCL8 Antibody Picoband®
Question
I have a question about product PA1353, anti-IL8/CXCL8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1353 anti-IL8/CXCL8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-09
Question
I see that the anti-IL8/CXCL8 antibody PA1353 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-24
Question
Will anti-IL8/CXCL8 antibody PA1353 work for IHC with periodontal ligament?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
According to the expression profile of periodontal ligament, IL8 is highly expressed in periodontal ligament. So, it is likely that anti-IL8/CXCL8 antibody PA1353 will work for IHC with periodontal ligament.
Boster Scientific Support
Answered: 2019-08-22
Question
We are currently using anti-IL8/CXCL8 antibody PA1353 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-23
Answer
The anti-IL8/CXCL8 antibody (PA1353) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-23
Question
Will anti-IL8/CXCL8 antibody PA1353 work on monkey WB with periodontal ligament?
Verified Customer
Verified customer
Asked: 2018-07-16
Answer
Our lab technicians have not tested anti-IL8/CXCL8 antibody PA1353 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-IL8/CXCL8 antibody PA1353 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey periodontal ligament in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-16
