Anti-C-C chemokine receptor type 4 CCR4 Antibody Picoband®

CCR4 antibody

Boster Bio Anti-C-C chemokine receptor type 4 CCR4 Antibody catalog # PA1517. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 1 publication(s).

Product Info Summary

SKU: PA1517
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-C-C chemokine receptor type 4 CCR4 Antibody Picoband®

View all CCR4 Antibodies

SKU/Catalog Number

PA1517

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-C-C chemokine receptor type 4 CCR4 Antibody catalog # PA1517. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-C-C chemokine receptor type 4 CCR4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1517)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of mouse CCR4, identical to the related rat sequence.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1517 is reactive to Ccr4 in Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

41 kDa

Calculated molecular weight

41408 MW

Background of CCR4

CCR4,C-C chemokine receptor type 4 is a protein that in humans is encoded by the CCR4 gene. CCR4 has also recently been designated CD194 (cluster of differentiation 194). The protein encoded by this gene belongs to the G protein-coupled receptor family. It is a receptor for the following CC chemokines. The CCR4 gene is mapped to chromosome 3. Chemokines are a group of small structurally related proteins that regulate cell trafficking of various types of leukocytes. The chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PA1517 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat

Positive Control

WB: Rat Thymus Tissue, Rat Spleen Tissue, HEPA Whole Cell

Validation Images & Assay Conditions

Gene/Protein Information For Ccr4 (Source: Uniprot.org, NCBI)

Gene Name

Ccr4

Full Name

C-C chemokine receptor type 4

Weight

41408 MW

Superfamily

G-protein coupled receptor 1 family

Alternative Names

C-C chemokine receptor type 4;C-C CKR-4;CC-CKR-4;CCR-4;CCR4;CD194;Ccr4;Cmkbr4; CCR4 CC-CKR-4, CD194, CKR4, CMKBR4, ChemR13, HGCN:14099, K5-5 C-C motif chemokine receptor 4 C-C chemokine receptor type 4|C-C CKR-4|CCR-4|chemokine (C-C motif) receptor 4|chemokine (C-C) receptor 4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Ccr4, check out the Ccr4 Infographic

Ccr4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Ccr4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1517 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Zhao C, Bu X, Wang W, Ma T, Ma H. Plos One. 2014 Jan 8;9(1):E85058. Doi: 10.1371/Journal.Pone.0085058. Ecollection 2014. Gec-Derived Sfrp5 Inhibits Wnt5A-Induced Macrophage Chemotaxis And Activation.

Have you used Anti-C-C chemokine receptor type 4 CCR4 Antibody Picoband®?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For Anti-C-C chemokine receptor type 4 CCR4 Antibody Picoband®

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

4 Customer Q&As for Anti-C-C chemokine receptor type 4 CCR4 Antibody Picoband®

Question

We are currently using anti-CCR4 antibody PA1517 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-14

Answer

The anti-CCR4 antibody (PA1517) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-14

Question

I am looking for using your anti-CCR4 antibody for immune response studies. Has this antibody been tested with western blotting on rat thymus tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-10-23

Answer

Thanks for your inquiry. This PA1517 anti-CCR4 antibody is validated on rat thymus tissue, tissue lysate, spleen tissue, hepa whole cell lysate. It is guaranteed to work for WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-10-23

Question

Our lab were happy with the WB result of your anti-CCR4 antibody. However we have seen positive staining in spleen cell membrane using this antibody. Is that expected? Could you tell me where is CCR4 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-04-25

Answer

Based on literature, spleen does express CCR4. Generally CCR4 expresses in cell membrane. Regarding which tissues have CCR4 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Spleen, Pubmed ID: 7642634

Boster Scientific Support

Answered: 2019-04-25

Question

We have been able to see staining in mouse leukocyte. Are there any suggestions? Is anti-CCR4 antibody supposed to stain leukocyte positively?

D. Johnson

Verified customer

Asked: 2018-01-24

Answer

According to literature leukocyte does express CCR4. According to Uniprot.org, CCR4 is expressed in leukocyte, spleen, blood, among other tissues. Regarding which tissues have CCR4 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Spleen, Pubmed ID: 7642634

Boster Scientific Support

Answered: 2018-01-24

Order DetailsPrice
PA1517

100μg

$370
PA1517-10ug

10μg sample (liquid)

$99
PA1517-Biotin

100 μg Biotin conjugated

$570
PA1517-Cy3

100 μg Cy3 conjugated

$570
PA1517-Dylight488

100 μg Dylight488 conjugated

$570
PA1517-Dylight550

100 μg Dylight550 conjugated

$570
PA1517-Dylight594

100 μg Dylight594 conjugated

$570
PA1517-FITC

100 μg FITC conjugated

$570
PA1517-HRP

100 μg HRP conjugated

$570
PA1517-APC

100 μg APC conjugated

$670
PA1517-PE

100 μg PE conjugated

$670
PA1517-iFluor647

100 μg iFluor647 conjugated

$670
PA1517-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
In stock
Order Product
PA1517
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.