Product Info Summary
SKU: | PA1517 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-C-C chemokine receptor type 4 CCR4 Antibody Picoband®
SKU/Catalog Number
PA1517
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-C-C chemokine receptor type 4 CCR4 Antibody catalog # PA1517. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-C-C chemokine receptor type 4 CCR4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1517)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse CCR4, identical to the related rat sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1517 is reactive to Ccr4 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
41 kDa
Calculated molecular weight
41408 MW
Background of CCR4
CCR4,C-C chemokine receptor type 4 is a protein that in humans is encoded by the CCR4 gene. CCR4 has also recently been designated CD194 (cluster of differentiation 194). The protein encoded by this gene belongs to the G protein-coupled receptor family. It is a receptor for the following CC chemokines. The CCR4 gene is mapped to chromosome 3. Chemokines are a group of small structurally related proteins that regulate cell trafficking of various types of leukocytes. The chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1517 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: Rat Thymus Tissue, Rat Spleen Tissue, HEPA Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-CCR4 antibody, PA1517, Western blotting
All lanes: Anti CCR4 (PA1517) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 50ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Lane 3: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 41KD
Observed bind size: 41KD
Protein Target Info & Infographic
Gene/Protein Information For Ccr4 (Source: Uniprot.org, NCBI)
Gene Name
Ccr4
Full Name
C-C chemokine receptor type 4
Weight
41408 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
C-C chemokine receptor type 4;C-C CKR-4;CC-CKR-4;CCR-4;CCR4;CD194;Ccr4;Cmkbr4; CCR4 CC-CKR-4, CD194, CKR4, CMKBR4, ChemR13, HGCN:14099, K5-5 C-C motif chemokine receptor 4 C-C chemokine receptor type 4|C-C CKR-4|CCR-4|chemokine (C-C motif) receptor 4|chemokine (C-C) receptor 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ccr4, check out the Ccr4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ccr4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-C-C chemokine receptor type 4 CCR4 Antibody Picoband® (PA1517)
Hello CJ!
PA1517 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhao C, Bu X, Wang W, Ma T, Ma H. Plos One. 2014 Jan 8;9(1):E85058. Doi: 10.1371/Journal.Pone.0085058. Ecollection 2014. Gec-Derived Sfrp5 Inhibits Wnt5A-Induced Macrophage Chemotaxis And Activation.
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Customer Q&As
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4 Customer Q&As for Anti-C-C chemokine receptor type 4 CCR4 Antibody Picoband®
Question
We are currently using anti-CCR4 antibody PA1517 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
The anti-CCR4 antibody (PA1517) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-14
Question
I am looking for using your anti-CCR4 antibody for immune response studies. Has this antibody been tested with western blotting on rat thymus tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
Thanks for your inquiry. This PA1517 anti-CCR4 antibody is validated on rat thymus tissue, tissue lysate, spleen tissue, hepa whole cell lysate. It is guaranteed to work for WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-23
Question
Our lab were happy with the WB result of your anti-CCR4 antibody. However we have seen positive staining in spleen cell membrane using this antibody. Is that expected? Could you tell me where is CCR4 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-04-25
Answer
Based on literature, spleen does express CCR4. Generally CCR4 expresses in cell membrane. Regarding which tissues have CCR4 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Spleen, Pubmed ID: 7642634
Boster Scientific Support
Answered: 2019-04-25
Question
We have been able to see staining in mouse leukocyte. Are there any suggestions? Is anti-CCR4 antibody supposed to stain leukocyte positively?
D. Johnson
Verified customer
Asked: 2018-01-24
Answer
According to literature leukocyte does express CCR4. According to Uniprot.org, CCR4 is expressed in leukocyte, spleen, blood, among other tissues. Regarding which tissues have CCR4 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Spleen, Pubmed ID: 7642634
Boster Scientific Support
Answered: 2018-01-24